齊一生物巨噬細(xì)胞炎性蛋白5(MIP-5)檢測試劑盒齊一生物科技有限公司產(chǎn)品的新老客戶,可享免費(fèi)代測實驗服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報價,可齊一生物科技有限公司.全國:歡迎選購!
齊一生物巨噬細(xì)胞炎性蛋白5(MIP-5)檢測試劑盒標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
操作步驟:
1.標(biāo)準(zhǔn)品的稀釋與加樣:在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在*、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在*、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻;然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻;然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻;混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉。(稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L)。Pierce PA127629 PTPRO 50 μg
Pierce PA127631 PTPRT 50 μg
Pierce PA127632 PTPRZ2 50 μg
Pierce PA127633 PTPRS 50 μg
Pierce PA127634 SIRPA 50 μg
Pierce PA127635 PTPN1 50 μg
Pierce PA127636 PTPN3 50 μg
Pierce PA127638 Ptpn11 50 μg
Pierce PA127639 PTPN12 50 μg
Pierce PA127640 PTPN13 50 μg
Pierce PA127641 PTPN14 50 μg
Pierce PA127642 PTPN22 50 μg
Pierce PA127643 DUSP8 50 μg
Pierce PA127644 MKP4 50 μg
Pierce PA127645 DUSP11 50 μg
Pierce PA127646 PTP4A2 50 μg
Pierce PA127647 cdc14 50 μg
Pierce PA127648 cdc14 50 μg
Pierce PA127649 cdc14 50 μg
Pierce PA127651 MEP1A 50 μg
Pierce PA127652 MEP1B 50 μg
Pierce PA127653 LAIR1 50 μg
Pierce PA127654 DAPP1 50 μg
Pierce PA127655 MTMR2 50 μg
Pierce PA127656 ACP1 50 μg
Pierce PA127658 DUSP23 50 μg
Pierce PA127660 CHEK2 50 μg
Pierce PA127661 PSTPIP2 50 μg
Pierce PA127663 PARG1 50 μg
Pierce PA127664 FCRL2 50 μg
Pierce PA127665 TNK2 50 μg
Pierce PA127666 DUSP15 50 μg
Pierce PA127667 RCP 50 μg
Pierce PA127668 C17orf75 50 μg
Pierce PA127669 LMAN1 50 μg
Pierce PA127670 SLC9A3R1 50 μg
Pierce PA127671 AMD1 50 μg
Pierce PA127672 YWHAE 50 μg
Pierce PA127673 RFC3 50 μg
Pierce PA127674 SUGT1 50 μg
Pierce PA127675 HSF2BP 50 μg
Pierce PA127676 PPIH 50 μg
Pierce PA127677 NOLA1 50 μg
Pierce PA127678 NDUFA10 50 μg
Pierce PA127679 ZNF395 50 μg
Pierce PA127681 GBL 50 μg
Pierce PA127682 rTS 50 μg
Pierce PA127683 rTS 50 μg
Pierce PA127684 MGMT 50 μg
Pierce PA127685 RNF113A 50 μg
Pierce PA127686 CARD14 50 μg
Pierce PA127687 CARD14 50 μg
Pierce PA127688 HAGH 50 μg
Pierce PA127689 SGK 50 μg
Pierce PA127691 DCTD 50 μg
Pierce PA127693 CCBL1 50 μg
Pierce PA127694 GNB4 50 μg
Pierce PA127695 SPOP 50 μg
Pierce PA127697 MTHFD2 50 μg
Pierce PA127699 ACAD-8 50 μg
Pierce PA127700 HOXC11 50 μg
Pierce PA127701 TTC4 50 μg
Pierce PA127702 GTF2A2 50 μg
Pierce PA127703 GTF2H1 50 μg
Pierce PA127705 TSTA3 50 μg
Pierce PA127707 GADD45G 50 μg
Pierce PA127708 COASY 50 μg
Pierce PA127709 Acetyl-CoA 50 μg
Pierce PA127710 STC2 50 μg
Pierce PA127711 COX4NB 50 μg
Pierce PA127712 BMZF3 50 μg
Pierce PA127713 TESS 50 μg
Pierce PA127714 ADI1 50 μg
Pierce PA127715 SH3BGR 50 μg
Pierce PA127716 GSTZ1 50 μg
Pierce PA127720 UPP1 50 μg
Pierce PA127721 RPC3 50 μg
Pierce PA127723 PSMA1 50 μg
Pierce PA127724 TNFAIP1 50 μg
Pierce PA127725 NMT2 50 μg
Pierce PA127726 CDKN2C 50 μg
Pierce PA127728 DRES93 50 μg
Pierce PA127729 EBP1 50 μg
Pierce PA127730 PSMC2 50 μg
Pierce PA127732 LMCD1 50 μg
Pierce PA127736 NDUFS3 50 μg
Pierce PA127737 CINP 50 μg
Pierce PA127738 RAB4A 50 μg
Pierce PA127739 PDAP1 50 μg
Pierce PA127740 TSSC1 50 μg
Pierce PA127743 CTSH 50 μg
Pierce PA127744 NNMT 50 μg