人細胞周期素D1(Cyclin-D1)ELISA檢測試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個試劑盒都經(jīng)過嚴格的質(zhì)檢控制,包括:板內(nèi)、板間實驗精度,靈敏度,回收率,穩(wěn)定性等指標(biāo)的測定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測體系簡單而直接,可根據(jù)450 nm波長的讀值迅速計算定量結(jié)果。試劑盒中提供的檢測抗體能夠識別全長靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測結(jié)果更穩(wěn)定可靠
人細胞周期素D1(Cyclin-D1)ELISA檢測試劑盒標(biāo)本要求:
1.標(biāo)本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
注意事項:
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準(zhǔn)。
計算:
以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),
在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD
值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)
準(zhǔn)曲線的直線回歸方程式,將樣品的OD值
代入方程式,計算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實際濃度。
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)與批見應(yīng)分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個月 齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,購買我公司產(chǎn)品的新老客戶,可享免費代測實驗服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本。全程提供。如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議。Instrument Part 9019400 Sensor, infrared, RG3
Instrument Part 9019407 Switch, mains voltage selector, RG3
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Instrument Part 9019438 Fan 115VAC 50/60 Hz 0.26A
9019519 ZLV ADAPTER KIT HIGH SKIRT
9019525 UV support frame, QIA
9019527 PLATE SEPARATOR 96 WELL WHITE
Instrument Part 9020640 Baseboard, front, AS, Cabinet Qsym
Instrument Part 9020639 Baseboard, front, SP, Cabinet Qsym
Instrument Part 9020365 Optical sensor, AS, Qsym
1061107 PG AIV-H9 RT-PCR Assay (48) Z
Instrument Part 9022438 USB Ethernet Adaptor, PyroQ96
Consumable 929564 #QX Pro Marker 14-97 kDa (575 μg)
Consumable 270805 cattletype MAP Ab (20)
Consumable 274202 flocktype IBDV Ab (2)
Consumable 273001 pigtype Salmonella Ab (1)
Consumable 204143 QuantiTect SYBR Green PCR Kit (200) For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect SYBR Green PCR Master Mix,* 2 x 2.0 ml RNase-free water
Consumable 33203 pQE-30Xa Vector DNA 25 ug pQE-30 Xa Vector DNA
Consumable 33213 Xa Removal Resin (2 x 5ml) 2 x 2.5 ml Xa Removal Resin, 3 x 1.9 ml 1 M Tris-Cl, pH 8.0
Instrument Part 9235294 Seal, rotary shaft, 10 x 16 mm
Instrument Part 9013444 Reagent holder, 3-trough 20 ml
Instrument Part 9011119 Valve, 3-way, dilutor, BR8000
Instrument Part 9011216 AC box, BR8000
Instrument Part 9011667 Side panel, technical tower, BR8000
Instrument Part 9011704 Cable, PCON98 to AC-box (L1), BR8000
Instrument Part 9011862 Worktable right, BR8000 v1.01
Instrument Part 9012391 Bottle, system liquid, 10 l, BR8000
MDx 4510035 artus Enterovirus LC RT-PCR Kit (96) PRE
Consumable 1026901 MagAttract Suspension G (13/15),KG 13 ml volume of suspension G
9019675 Notebook, XP
Consumable 950183 Tissue Digest Reagent (DXT) (250 ml)
Instrument Part 990512 Filter-Tips, 50 μl (960)
9021866 Hinge, Lid, Metal housing, QGX
Consumable 970580 RAS Extension Pyro Kit (24)
Consumable 12281 QIAfilter Plasmid Mega Kit (5) 5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Consumable 12191 QIAGEN Plasmid Giga Kit (5) 5 QIAGEN-tip 10000, Reagents, Buffers
Consumable 74104 RNeasy Mini Kit (50) 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable 120025 TurboFilter 96 Plates (96) 96 TurboFilter 96 Plates
Accessory 19407 VacConnectors (500) 500 disposable connectors for use with QIAamp spin columns on luer connectors. Should be ordered in conjuntion with 19408 - VacValves (24).
Consumable 34705 6xHis Protein Ladder 6xHis-tagged marker proteins (lyophilized, for 50-100 lanes on western blots)
Instrument Part 9017698 Maintenance Kit, MultiFlow, BR UNIV
Instrument Part 9239076 User Manual, BS Software 1.0
Instrument Part 9240507 Software & Std. Protocols, AP196
Instrument Part 9240642 Cable Assy, Proteinase K Pump, AP196/98
Instrument Part 9240727 Tube Assy, Vacuum Manifold, AP96/196
Instrument Part 9240691 Shoulder Bolt, Spindle Retainer, AP
Instrument Part 9240771 Assy, Centrifuge Rotor Locator, AP96
Instrument Part 9018126 Cover, extractor, Qsym
Instrument Part 9018145 Adapter, heated shaker, Qsym
Instrument Part 9018173 PCB, connector, portal, Qsym
Instrument Part 9240946 Encoder, y, portal, Qsym
Instrument Part 9240956 Cable, handler, Qsym
Instrument Part 9240979 Cable, sample tracking, Qsym
Replacement Component 1067930 Buffer ALO (10 ml)
Replacement Component 1068797 Buffer DLB (13mg lyophylized), G
Consumable 387016 Investigator Quantiplex Kit (200)
Consumable 288315 cador Salmonella PCR Reagent (96)
Consumable 979102 PyroMark Q96 HS Q96 Reagent Tip (4) Re-usable tips for dispensing reagents (RDTs) for use in PyroMark Q96 MD. Each package contains 4 tips.
Instrument Part 990550 Tip Disposal Box (10)
Instrument Part 990556 Reagent Trough Lid 170 mL (20)
Consumable 950921 Capture Plate, VX (5)
337012 FDK design fee
4838024 careNG PCR ASSAY V2
Consumable 870601 T315I RGQ PCR Kit (24)
Instrument Part 9022656 Channeling Adapter, QXT
Consumable 10023 QIAGEN-tip 20 (25) 25 columns; 20 μg column capacity
Consumable 19056 Buffer QF 1000 ml Elution Buffer
Consumable 19054 Buffer QBT 1000 ml Equilibration Buffer
Consumable 30210 Ni-NTA Agarose (25 ml) 25 ml nickel-charged resin (max. pressure: 2.8 psi)
Consumable 51306 QIAamp DNA Mini Kit (250) For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)
Consumable 19077 Buffer AE 240 ml Elution Buffer for 1000 preps
Accessory 19131 QIAGEN Proteinase K (2) 2 ml (>600 mAU/ml, solution)
Accessory 19566 Collection Microtube Caps Nonsterile polypropylene caps for collection microtubes (1.2 ml) and round-well blocks, 960 in strips of 8
Accessory 19579 Flat-Bottom Blocks (24) 96-well blocks with 2 ml wells: 24 per case, lids included
Consumable 19585 S-Blocks (24) "Succsessor of
#19573 Square-Well Blocks (24)"
Consumable 28006 MinElute PCR Purification Kit (250) 250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Consumable 28183 QIAquick 96 PCR Purification Kit (24) For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps
9017849 Swing-out Buckets (2)
Instrument Part 9240630 Power Supply, 5VDC 1.5 AMP, AP96
Instrument Part 9240663 Nut, Flangeless,1/4-28, Black, AP
Instrument Part 9240678 Bimba Rotary Actuator, AP
Instrument Part 9018129 Belt, x, extractor, Qsym
Instrument Part 9018150 PCB, interface, drawer, Qsym
Instrument Part 9240949 Cable, y, portal, Qsym
Instrument Part 9240960 Cable, cLLD, pipetting system, Qsym
Consumable 1062791 HBV PCR REAGENT III
Instrument Part 9243045 Bracket, rear, transparent panel, Qsym
Consumable 292143 mericon Horse Kit (24)
Instrument Part 9019074 PyroMark Storage Box 13x8x5cm, Pyro Box for dust free and safe storage of tips in-between runs in PyroMark Q96 MD.
Consumable 9019077 PyroMark Assay Design SW 2.0 All-in-one software for design of both PCR and sequencing primers
Consumable 979012 Fungi ITS2 Primers For PCR
Consumable 979301 PyroMark Q24 Plate (100) CE 24 well plate for samples, for use in PyroMark Q24. 100 plates in each package. CE-marked
Accessory 19505 QIAvac HiSpeed LS
Consumable 990602 Elution Plate (10)
Consumable 206672 Type-it CNV SYBR Green PCR +qC Kit (100)
Consumable 10083 QIAGEN-tip 2500 (25) 25 columns; 2.5 mg column capacity
Consumable 12143 QIAGEN Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers
Consumable 19052 Buffer P2 500 ml Lysis Buffer
Consumable 12245 QIAfilter Plasmid Midi Kit (100) 100 QIAGEN-tip 100, Reagents, Buffers, 100 QIAfilter Midi Cartridges
Consumable 27106 QIAprep Spin Miniprep Kit (250) For 250 high-purity plasmid minipreps: 250 QIAprep Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
Accessory 81031 Plate Rotor 2 x 96 Rotor for 2 QIAGEN 96-well plates, for use with QIAGEN Centrifuges
Consumable 19065 Buffer PE (concentrate) 100 ml Wash Buffer (5x concentrate for 500 ml buffer)
Consumable 19082 Buffer ASL (4 x 140 ml) 560 ml Stool Lysis Buffer
Consumable 20021 QIAEX II Gel Extraction Kit (150) For 150 extractions: 3 x 0.5 ml QIAEX II Suspension, Buffers
Consumable 28106 QIAquick PCR Purification Kit (250) For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
Consumable 31014 Ni-NTA Spin Columns (50) 50 Ni-NTA Spin Columns, Collection Tubes
Consumable 32149 QIAexpress Type IV Kit 5 ug each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose. pQE-40
Consumable 34650 RGS·His Antibody, BSA-free (100 μg) 100 ug mouse anti-RGS(H)4 (lyophilized, BSA-free, for 1000 ml working solution)
Instrument Part 9240517 Cable, Dual, USB transition, AP196/98
Instrument Part 9240632 Power Supply, 48VDC 4.0 AMP, AP96