人抗腦組織抗體(ABAb)ELISA檢測(cè)試劑盒本公司新品*,本公司長(zhǎng)期經(jīng)營(yíng)大鼠ELISA試劑盒、人elisa試劑盒,小鼠elisa試劑盒等種屬試劑盒,歡迎您訪問我們的,www.qiyibio。。com全國(guó):歡迎選購(gòu)!如有什么問題請(qǐng)給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議
產(chǎn)品名稱:人抗腦組織抗體(ABAb)ELISA檢測(cè)試劑盒
規(guī)格:96T/48T
檢測(cè)標(biāo)本:血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測(cè)方法:ELISA
檢測(cè)類型:酶聯(lián)免疫夾心法
運(yùn)輸保存:2~8°保存6個(gè)月
齊一生物主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒全國(guó):,種類齊全,價(jià)格實(shí)惠,購(gòu)買齊一生物產(chǎn)品的新老客戶,可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù),可檢測(cè)人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報(bào)價(jià),可齊一生物.
特點(diǎn)
一、高效、靈敏、特異的抗體;
二、穩(wěn)定的重復(fù)性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相載體;
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟:
1)抗原表位的計(jì)算機(jī)篩選;
2)抗原的制備;
3)血清的采集;
4)間接ELISA方法的建立;
5)間接ELISA方法的敏感性和特異性驗(yàn)證;
6)試劑盒的穩(wěn)定性驗(yàn)證;
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清:操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿:EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液:1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿:將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存:如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
此IBL試劑盒能用于小鼠血清,EDTA血漿,細(xì)胞上清中產(chǎn)品定量檢測(cè)試劑盒成分
1預(yù)包被板:抗小鼠白介素-6兔子IgG,親合純化96T
2酶標(biāo)記抗體:(30倍濃縮)HRP標(biāo)記抗小鼠白介素-6兔子IgG,親合純化0.4mLx1
3標(biāo)準(zhǔn)品:重組小鼠白介素-60.5mLx2
4EIA緩沖液:含1%BSA,0.05%吐溫20BPS30mLx1
5標(biāo)記抗體稀釋液:含1%BSA,0.05%吐溫20BPS12mLx1
6顯色劑:TMB底物液15mLx1
7終止液:1N硫酸12mLx1
8濃縮洗滌液:(40倍濃縮)含1%BSA,0.05%吐溫20BPS50mLx1操作說明1實(shí)驗(yàn)所需器材(但試劑盒沒有提供)酶標(biāo)儀(450nm)微移液管及其吸嘴量筒及燒杯去離子水冰箱(4°C)坐標(biāo)紙(log/log)吸水紙?jiān)嚬?用于標(biāo)準(zhǔn)品稀釋)溫育箱(37°C±1°C)洗瓶(用于洗板)一次性試劑管(用于濃縮酶標(biāo)記抗體和顯色劑)
9019401 Connector, COM incl. int. cable, RG3
9019360 Switch, mains voltage selector, RG6
1027417 Flexitube siRNA 5 nmol
9019339 LED, green (530), RG6
9019331 EPROM, IC Motherbrd, state FW, RG6
9019237 Linear guide - X, PyroQ24
9234673 X-Assembly Turntable Card
9234659 Cassette, HSPS, click'n'go
1026956 Buffer AVE (Elution Buffer) (125/125),KG
1026905 RNeasy 96 Plates (12)
9233674 Probe, ceramic coat., 0.4mm, 158mm
9233630 Mousepad BR 01/10 (WW)
9019209 Motor - Gripper, PyroQ96MD
9019197 Damper kit - Robot, PyroQ96MD
1020955 Buffer AW2 with Azid (127/500),KG
1062791 HBV PCR REAGENT III
9232903 Spritzenpumpe mMBP 2021C P7xxx
1061065 PG Influenza A RT-PCR Assay (48) Z
9232900 Valve, release, auto vac, BR8000
9016710 Software Package LiquiChip Analyzer
9019891 Pipetting head, 8 ch. , CX1
9019881 Lid with LED, red, RG6
9019857 36-well High Profile Locking Ring
9019837 IPC Hard Drive PCA6751, PyroQ96MD
9022832 Notebook_V3 W7_32bit
9013703 Tubing, syringe to connector, BR3000
9013485 Membrane, Simritz (20)
9238805 Round sealing cap, clear
9019637 Cable, UV lamp volt. conv., long, EZ1xl
9019590 Cover, plexiglas, 8 ch., CX1
9019565 Clamp, cable 2 wires 2 tabs, Cxx
9019543 Bow spring, table rail, Cxx
9019510 CHAMBER MAIN VAC STATION 1820
9019505 PLATE SEPARATOR 48 WELL HIGH
204256 QuantiFast Probe PCR Kit (2000)
204174 Rotor-Gene SYBR Green RT-PCR Kit (400) The Rotor-Gene SYBR Green RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time one-step RT-PCR using SYBR Green I.
204154 QuantiFast SYBR Green RT-PCR Kit (400)
9019424 Wiring, lower heater, RG3
9019417 Fan, lid, RG3
9019393 Solenoid, cooling with flap, RG3
9019386 Photo Multiplier Tube with cable, RG3
9019368 Damper, lid, RG6
9019347 Motor, 12VDC Heater fan, RG6
9234697 Manual Hardware Plato 7
9020477 Gasket, front plate, GSC
9234463 Coupling, CPC, inline, female, 8mm
1027285 AllStars Neg. siRNA AF 546 (5 nmol)
1022564 Mm/Hs_MAPK1 control siRNA (5 nmol)
9019205 Opto - Stacker, PyroQ96MD
9019204 Opto - Robot Z motor , PyroQ96MD
9019200 Hinge kit - Top cover, PyroQ96MD
1022073 Luciferase GL3 siRNA (5 nm)
1022064 GFP-22 siRNA (5 nm)
1018996 PCR Buffer, 10 x (22ml/30),KG
1064605 MagAttract M48 DNA Manual Kit (200)
1066851 PG FMDV RT-PCR Assay (48) LE Z
1061054 HBV PCR REAGENT I
1061069 UU PCR REAGENT II
1061079 NG PCR REAGENT II
9019886 Lid, label 4CH, RG3
9019875 Cover, main, silver, RGQ
9019836 IPC Hard Drive PCA6751, PyroQ96ID
9019769 Valve, shutoff, V2, QX
9019756 Valve, solenoid, V2, QX
9019734 Sensor, Z, with cable, V2, QX
9019728 Board, motor control, Y, V2, QX
9013832 Reagent Holder, 1-bottle Holder for acmodating 1 bottle (5 ml). For use with QIAGEN Protease on the BioRobot MDx worktable
9013824 BGR Tischplatte P3041 Robotec(SN602)
9013719 RS 232 Servicecabel
9013506 96-well Thermostat Base Plate1.02
9013484 Cooling and heating system, BR3000
9013442 Centrifuge, strip adaptor set
9013434 Maintenance kit, annual, BR9600 (A)
9019723 Transport lock, EZ1xl
9019656 Hose w. quick lock socket, PyroQ96VW
9019581 Pulley, Idler Y-axis, Cxx
9019554 Rail, Z-axis 138 mm, Cxx
9019552 Rail, X-axis 444 mm, CQx
218060 miScript Reverse Transcription Kit (10)
216513 FastLane Cell Multiplex Kit (200)
204254 QuantiFast Probe PCR Kit (400)
9019440 Solid State Relay
9019433 Transformer 2x115VAC O/P 2x11VAC
9019431 Kit, lock ring 36W incl. lockknob, RG3
9019402 Transformer, heater, RG3
1027321 Mm/Hs_MAPK1 control siRNA (20 nmol)
9019329 Serial connector USB, internal cable, RG
9019238 Heating block, PyroQ24
9020478 Heating plate, GSC
1026965 PCR Buffer,10 x (1,2/2) without MgCl2
9233466 Coupling, CPC, inline, male, 6mm
9233452 Coupling, X motor, BR8000
9019217 Lead screw - Y robot, PyroQ96MD
1017635 Protease Solvent 6x (10,2/15), Azid,KG
1066846 AIV-H5 RT-PCR REAGENT LE
1054250 Effectene Transfection Reag. Kit (0.3ml)
1061051 HBV PCR REAGENT II
9019890 Foam insert, rotor securing, QIAC
9019861 Rotor Locking Nut Set
9019821 IQ/OQ document, QS
9019768 Sensor, interlock2, with cable, QX
9019765 Belt, Y, V2, QX
9019727 Board, transport conversion, V2, QX
9013523 Underlay, vaccum manifold, v2.01
9013447 Control unit Y/Z, 4 channel
9013431 Maintenance kit, semi annual, BR9600 (E)
9238807 Round carrier for 12 Tubes 15ml
9019715 PyroMark Q24 User Manual