異質(zhì)型核糖核蛋白復(fù)合物抗RA33抗體（hnRNPRA33）ELISA檢測(cè)試劑盒

異質(zhì)型核糖核蛋白復(fù)合物抗RA33抗體（hnRNPRA33）ELISA檢測(cè)試劑盒齊一生物您實(shí)驗(yàn)的好伙伴為您提供“zui高品質(zhì)的產(chǎn)品”和“的服務(wù)”
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試劑盒名稱：異質(zhì)型核糖核蛋白復(fù)合物抗RA33抗體（hnRNPRA33）ELISA檢測(cè)試劑盒
規(guī)格：48T/96T
品牌：進(jìn)口/國(guó)產(chǎn)
產(chǎn)品的用途：僅供科研究使用！
價(jià)格：全國(guó)：,到我司741653262,齊一生物科技（上海）有限公司提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒,性價(jià)比*.
簡(jiǎn)介：
ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到*科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè).
2用途
elisa技術(shù)流程
ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記.結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性,酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性.在測(cè)定時(shí),受檢標(biāo)本（測(cè)定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應(yīng).用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開.再加入酶標(biāo)記的抗原或抗體,也通過(guò)反應(yīng)而結(jié)合在固相載體上.此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例.加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應(yīng)的結(jié)果,使測(cè)定方法達(dá)到很高的敏感度.ELISA可用于測(cè)定抗原,也可用于測(cè)定抗體.
然而,影響Elisa試驗(yàn)結(jié)果的因素很多,故加強(qiáng)各個(gè)環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn).
試驗(yàn)原理
試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.
原理
免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).
HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml
ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與*次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

28304    QIAquick Nucleotide Removal Kit (50)    50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
9012730    Cable, flat band, X, 2300 mm
9013173    Pump, probe flush, BR8000
4510033    artus Enterovirus LC RT-PCR Kit (24) PRE
130718    NeXtal Tubes PACT Suite
19088    Reagent DX
218411    Human miScript Primer Assay Set VXX.X
9019899    Cable - Ground, PyroQ96ID
9019003    Hinge, for door, EZ1xl
9019004    Grid, UV lamp, EZ1xl
9019021    Damper, door, EZ1xl
9019759    Power supply, main, 24V, V2, QX
950154    Wash Reagent (DXW) (650 ml)
9019537    Plate Stacker A/B RD, RCS
9021031    Frame, transfer module, Qsym
337042    qBiomarker Assay PreAMP Mix
51161    QIAamp 96 DNA Blood Kit (4)    For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels
75162    RNeasy Maxi Kit (12)    12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers
120016    QIAquick 96 Plates (24)    Individual QIAquick 96 plates and handbook. No buffers or reagents.
203443    HotStarTaq Master Mix Kit (250)    "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

- premixed solution for high PCR specificity
- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available"
301108    PolyFect Transfection Reagent (100 ml)    For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.
231222    QIAGEN PCR Cloning plus Kit (10)    For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 ul each), SOC medium (2 x 1.9 ml)
9015638    AC fuse, 115 V, 250 8A, M48
132568    NeXtal Stock Methoxy-PEG 5000 (50)    30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
9019577    Opto switch, Z-axis, 1 ch. , Cx2
9019594    Belt, Z-axis gearbox (black), 8 ch., Cx8
9019068    PyroMark CpG SW 1.0 (5)    "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies. 
Five additional software licenses for PyroMark CpG SW 1.0"
9019086    PyroMark Q96 MD Software (5)    "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.

5 licenses"
9019658    O rings - VPT Fast connector, PyroVW
972012    PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16
979003    PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.
979008    PyroMark Wash Buffer (conc., 200 ml)    For washing and neutralizing the immobilized DNA.
950140    Binding Additive (DX) (4.0 g)
9019803    Upgrade Package 1, RCS
130803    NeXtal CubicPhase 1w Mo μplate (10)
9243044    Set, brackets, transparent panel, Qsym
283615    virotype PEDV/TGEV RT-PCR Rgt (96)
52906    QIAamp Viral RNA Mini Kit (250)    For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183    DyeEx 96 Kit (24)    24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582    DNeasy 96 Blood & Tissue Kit (12)    For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042    Oligotex mRNA Midi Kit    For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523    Small Spin Columns    24 RNase-free spin columns for Oligotex spin procedures
301109    PolyFect Transfection Reagent (10x100ml)
9239361    PCB-micro-fuse T 6.3A, 250V, EZ1
132632    NeXtal Stock Potassium formate (50)    10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610    Cover, Pipettor, 1 ch. (white), CAS
9019624    PCB, lge breakout, 12 core to rev 3, Cx2
9019067    PyroMark CpG SW 1.0    Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087    PyroMark IdentiFire SW 1.0    Software for sequence-based species identification by paring and matching Pyrosequencing data to a local database.
972052    PyroMark Q96 CpG Prader Willi (96)    For quantification of methylation level in  Prader-Willi and Angelman Syndromes
979002    PyroMark Q96 Plate Low (100)    For sample analysis of DNA template on ID.
979004    PyroMark Q96 Cartridge (3)    For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513    PyroMark Q24 MDx    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610    Filter-Tips, OnCor C, 200 μl (960)
9017842    Tool, gauge, 30 mm, calibration, QIAC
9020806    Rotor Timing Calibration Dye, RGQ
331455    miScript Microfluidics PreAMP Kit (96)
381566    Investigator ESSplex SE GO! Kit (200)
9022654    Transfer Carriage QIAplate, QXT
16191    QIAwell 96 Ultra Plasmid Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046    Plasmid Buffer Set    Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201    Collection Tubes (2-ml)    1000 Collection Tubes (2 ml)
9015862    EZ1 DNA PRFN Card    Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163    MagAttract 96 DNA Plant Core Kit (24)    MagAttract Suspension A and buffers for 24 x 96 preps
1030483    Elution Microtubes CL, PAX, FLU
9232687    Hood f. Tip Disposal Station 2.01
9015972    Tube Holder, STS MDx, 15 mm (12)
158926    Cell Suspension Solution (125 ml)
159992    Generation DNA Purif. Soln 1 (1000 ml)
201913    dNTP Set, PCR Grade 100mM, (4x250μl)    dNTP Set is a plete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50ul PCR reactions
12945    QIAGEN Plasmid Plus Midi Kit (100)    "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
9019715    PyroMark Q24 User Manual
9019861    Rotor Locking Nut Set
9019890    Foam insert, rotor securing, QIAC
336391    SureSilencing shRNA Control
330522    RT2 SYBR Green ROX qPCR Mastermix (12)
330411    RT2 HT First Strand Kit (96)
336161    Multi-Analyte  ELISArray Kit
9020716    Optoswitch, X axis, C34
9020712    Rail, x axis, C34
9242544    Power Input Module, Eppendorf, AP196/98
9020713    Rail guide, x axis, C34
9020911    Foot assembly, SMC, QGX
9020679    Rail, left, CX1
9022916    Gas press.spring conversion kit QG/QXT
9012771    Liquid Handling Kit Plato 7382/7182
9013150    BGR Tischabstützung  Digene
9013205    BGR Labhand li+re Digene
9014878    Card, x-main, shaker
202605    HotStar HiFidelity Polymerase Kit (1000)    2 x 500 units HotStar HiFidelity DNA Polymerase, 5x PCR Buffer containing dNTPs, 5x Q-Solution, 25 mM MgSO4, RNase free water
9019368    Damper, lid, RG6
9019386    Photo Multiplier Tube with cable, RG3
9019393    Solenoid, cooling with flap, RG3
9019417    Fan, lid, RG3
9019424    Wiring, lower heater, RG3
9019446    Memory card 32MB SDl w/S/W
9019451    Switch incl. 2K2 resistor, P/R, HS
9019473    Loading guide, 52 well sharkstooth, GSC
9019505    PLATE SEPARATOR 48 WELL HIGH
9019510    CHAMBER MAIN VAC STATION 1820
9019543    Bow spring, table rail, Cxx
9019565    Clamp, cable 2 wires 2 tabs, Cxx
9242545    Quick Disconnect O-ring set, LC
9242337    Tool, calibration, AS, Qsym
9018092    Cooling adapter, RG strip tubes 72, Qsym
9020390    Kit, fuse AS, Qsym
4870004    PG Influenza A RT-PCR Kit (48) Z
9022321    RS-232 Cable (2), careHPV
273505    pigtype Trichinella Ab (20)
273401    pigtype Toxoplasma Ab (1)
270043    cattletype BHV1 gB Ab (5)
288103    bactotype Mycoplasma Mg/Ms PCR Kit (24)
9021234    Vacuum Pump (100 V), PyroQ24VW
9011151    Valve, dilutor, 4-port
204443    QuantiTect Probe RT-PCR Kit (200)    For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water
9011243    Holder, bar-code camera, STS, BR3000
9011501    Coupling, Z motor, buffer track, BR8000
9012390    Wash station, 4-probes v2.01
9012671    Cable, PCON98 to release valve, BR8000
4500265    artus HSV-1/2 RG PCR Kit (96) CE
130703    NeXtal Tubes Cryos Suite
132101    NeXtal DWBlock Adhesive Foils
9019509    Pinch Valve, C18
9019542    Gas Strut, lid, 145 N, CQx
9019563    Bracket, mounting Y-axis, Cxx
9020392    PCB, connect drawer, chassis AS, Qsym
9020633    Pos. aid (1), waste box, Cabinet Qsym
9020252    UPG Waste bag holder, Qsym
9242546    Probe Holder Assembly, LX200, LC
4872004    PG AIV-H5 RT-PCR Kit (48)  Z
1072538    HotStarTaq DNA Polymerase (5x5000)
9022474    Shipment Box, careHPV
619024    careBrush, CE
271906    cattletype Milk Prep Kit (50)
280437    virotype BTV RT-PCR Kit (480)
289980    intype IC-DNA (1ml)
1018013    Buffer RPE (55/250) N,KG
9230446    Probe, stainless steel, 0.9mm, 158 mm
310107    BioMag Goat Anti-Rat IgG (500ml)    BioMag goat anti-rat IgG secondary antibody suspension (1 mg/ml)
9010892    Gripper, left, handler, BR8000
9011730    Cable, level sensor, BR8000
9012392    Bottle, waste, 10 l, BR8000
9012714    Tubing set, auto vac, BR8000
9012803    Chuck, collet, probe, BR8000 v1.00
9013181    Tubing, pump outlet, DISU, BR8000
130715    NeXtal Tubes ProComplex Suite
130717    NeXtal Tubes ComPAS Suite
218300    miScript Primer Assay (100)
9019005    Door lock assembly, EZ1xl
9019019    Coil, EZ1xl
9019020    Coupling, Z axis, EZ1xl
9019914    PCB, HEPA filter, CAS
9019758    Interlock jumper (2), V2, QX