乙型肝炎病毒前S2抗體(HBVpreS2Ab)試劑盒齊一生物科技有限公司新品*,齊一生物科技有限公司長期經(jīng)營大鼠ELISA試劑盒,人elisa試劑盒,小鼠elisa試劑盒等種屬試劑盒,歡迎您訪問我們的,www.qiyibio。。com全國:歡迎選購!如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議
試劑盒名稱:乙型肝炎病毒前S2抗體(HBVpreS2Ab)試劑盒
規(guī) 格:48T/96T
品牌:進(jìn)口原裝/分裝/國產(chǎn)
產(chǎn)品的用途:僅供科研究使用!
價(jià)格:電議,咨詢在線客服,到我司qysw@qiyibio.。齊一生物科技(上海)有限公司提供大量品牌進(jìn)口原裝、以及穩(wěn)定性強(qiáng)的國產(chǎn)ELISA試劑盒,性價(jià)比*歡迎您前來選購!
操作步驟
實(shí)驗(yàn)開始前,請?zhí)崆芭渲煤盟性噭?,試劑或樣品稀釋時(shí),均需混勻,混勻時(shí)盡量避免起泡。每次檢測都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí),用樣品稀釋液進(jìn)行稀釋,以使樣品符合試劑盒的檢測范圍。
1. 加樣:分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測樣品孔??瞻卓准訕悠废♂屢?00μl,余孔分別加標(biāo)準(zhǔn)品或待測樣品100μl,注意不要有氣泡,加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻,酶標(biāo)板加上蓋或覆膜,37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性,每次實(shí)驗(yàn)請使用新的標(biāo)準(zhǔn)品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl(取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制),37℃,60分鐘。
3. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4. 每孔加辣根過氧化物酶標(biāo)記親和素工作液(同生物素標(biāo)記抗體工作液) 100μl,37℃,60分鐘。
5. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6. 依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內(nèi),此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色,后3-4孔梯度不明顯,即可終止)。
7. 依序每孔加終止溶液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性,底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8. 用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測。
Anti-OCT1/OTF1
Anti-GARP/LRRC32
Anti-MEIS1
Anti-MEIS1
Anti-DDX4
Anti-NA
Anti-MRF2
Anti-STING
Anti-SOX17
Anti-SOX9
Anti-Phospho-HDAC-7 pSer448
Anti-TCF19/SC1
Anti-Nestin
Anti-NKX2-5
Anti-BST1
Anti-GATA-4
Anti-ORC1L
Anti-SPZ1
Anti-OCTL1/OCT-1
Anti-PER2
Anti-HOXB4
Anti-HtrA1
Anti-TAG/TIRAP3b
Anti-CDX3/CDX2
Anti-DLX3
Anti-FLII
Anti-POU3F2
Anti-DVL-3
Anti-14-3-3 zeta
Anti-BSA
Anti-Brachyury
Anti-OTX2
Anti-HB9
Anti-LIN28
Anti-DVL-1
Anti-HOXA9
Anti-GLI1
Anti-Influenza A H1N1 NA
Anti-Influenza A H1N1 NA
Anti-FNBP1
Anti-hnRNPK
Anti-Mili
Anti-Musashi-1
Anti-ZBTB2
Anti-S1PR1
Anti-Lefty1/2
Anti-CDCA7L/RAM2
Anti-DPPA4
Anti-LRRFIP1
Anti-ZIC2
Anti-Presenilin enhancer protein 2
Anti-DLL3
Anti-SLAR
Anti-Histone H2A.x
Anti-RuvB-like 1
Anti-RuvB-like 1
Anti-DLL4
Anti-TRIL
Anti-NOTCH2
Anti-OLIG2
Anti-DLL1
03MR051K25 Magic Red? Cathepsin B Kit MAGIC RED? CATHEPSIN B KIT 25 T
03MR051K80 Magic Red? Cathepsin B Kit MAGIC RED? CATHEPSIN B KIT 100 T
03MR052K25 Magic Red? Cathepsin L Kit MAGIC RED? CATHEPSIN L KIT 25 T
03MR052K80 Magic Red? Cathepsin L Kit MAGIC RED? CATHEPSIN L KIT 100 T
03MR053K25 Magic Red? Cathepsin K Kit MAGIC RED? CATHEPSIN K KIT 25 T
03MR053K80 Magic Red? Cathepsin K Kit MAGIC RED? CATHEPSIN K KIT 100 T
03MSK10125 Mitochondrial Permeability Transition Detection Kit MITOCHONDRIAL PERMEABILITY TRANSITION DETECTION KIT 25 test
03MSK10180 Mitochondrial Permeability Transition Detection Kit MITOCHONDRIAL PERMEABILITY TRANSITION DETECTION KIT 100 test
蛋白激酶檢測試劑盒
03KK11101 Ak/Pak2 Assay Kit AK/PAK2 ASSAY KIT 1 kit
03KK11201 Ck-2 Assay Kit CK-2 ASSAY KIT 1 kit
03KK11301 Mbpk Assay Kit MBPK ASSAY KIT 1 kit
03KK11701 Cpk Assay Kit cPK ASSAY KIT 1 kit
03KK12501 Bckd Kinase Kit BCKD KINASE KIT 1 kit
03AKK14801 Anti-Pp2Ac Detection Kit ANTI-PP2Ac DETECTION KIT 1 kit
03AKK14901 Anti-I 1 Pp2A Detection Kit ANTI-I 1 PP2A DETECTION KIT 1 kit
03AKK15001 Anti-I 2 Pp2A Α Detection Kit ANTI-I 2 PP2A α DETECTION KIT 1 kit
03AKK15101 Anti-Ak/Pak2 Detection Kit ANTI-AK/PAK2 DETECTION KIT 1 kit
03AKK15201 Anti-Pak Detection Kit ANTI-PAK DETECTION KIT 1 kit
03AKK15301 Anti-eIF4E Detection Kit ANTI-eIF4E DETECTION KIT 1 kit
calpain抑制劑
03DK00705 Z-Leu-Leu-Tyr-Diazomethylketone Z-LEU-LEU-TYR-DIAZOMETHYLKETONE 5 mg
03DK00710 Z-Leu-Leu-Tyr-Diazomethylketone Z-LEU-LEU-TYR-DIAZOMETHYLKETONE 10 mg
03DK00725 Z-Leu-Leu-Tyr-Diazomethylketone Z-LEU-LEU-TYR-DIAZOMETHYLKETONE 25 mg
03FK01801 Mu-Val-Homophe-Fluoromethylketone MU-VAL-HOMOPHE-FLUOROMETHYLKETONE 1 mg
03FK01803 Mu-Val-Homophe-Fluoromethylketone MU-VAL-HOMOPHE-FLUOROMETHYLKETONE 3 mg
03FK01805 Mu-Val-Homophe-Fluoromethylketone MU-VAL-HOMOPHE-FLUOROMETHYLKETONE 5 mg
03FK01701 Z-Leu-Leu-Tyr-Fluoromethylketone Z-LEU-LEU-TYR-FLUOROMETHYLKETONE 1 mg
03FK01703 Z-Leu-Leu-Tyr-Fluoromethylketone Z-LEU-LEU-TYR-FLUOROMETHYLKETONE 3 mg
03FK01705 Z-Leu-Leu-Tyr-Fluoromethylketone Z-LEU-LEU-TYR-FLUOROMETHYLKETONE 5 mg
caspase抑制劑
03AL01005 Ac-Asp-Glu-Val-Asp-Aldehyde AC-ASP-GLU-VAL-ASP-ALDEHYDE 5 mg
03AL01010 Ac-Asp-Glu-Val-Asp-Aldehyde AC-ASP-GLU-VAL-ASP-ALDEHYDE 10 mg
03AL01025 Ac-Asp-Glu-Val-Asp-Aldehyde AC-ASP-GLU-VAL-ASP-ALDEHYDE 25 mg
03AL01205 Ac-Ile-Glu-Thr-Asp-Aldehyde AC-ILE-GLU-THR-ASP-ALDEHYDE 5 mg
03AL01210 Ac-Ile-Glu-Thr-Asp-Aldehyde AC-ILE-GLU-THR-ASP-ALDEHYDE 10 mg
03AL01225 Ac-Ile-Glu-Thr-Asp-Aldehyde AC-ILE-GLU-THR-ASP-ALDEHYDE 25 mg
03AL02205 Ac-Leu-Glu-His-Asp-Aldehyde AC-LEU-GLU-HIS-ASP-ALDEHYDE 5 mg
03AL02225 Ac-Leu-Glu-His-Asp-Aldehyde AC-LEU-GLU-HIS-ASP-ALDEHYDE 25 mg
03AL02605 Ac-Leu-Glu-Val-Asp-Aldehyde AC-LEU-GLU-VAL-ASP-ALDEHYDE 5 mg
03AL02625 Ac-Leu-Glu-Val-Asp-Aldehyde AC-LEU-GLU-VAL-ASP-ALDEHYDE 25 mg
03AL01305 Ac-Tyr-Val-Ala-Asp-Aldehyde AC-TYR-VAL-ALA-ASP-ALDEHYDE 5 mg
03AL01325 Ac-Tyr-Val-Ala-Asp-Aldehyde AC-TYR-VAL-ALA-ASP-ALDEHYDE 25 mg
03AL00905 Ac-Val-Ala-Asp-Aldehyde AC-VAL-ALA-ASP-ALDEHYDE 5 mg
03AL00925 Ac-Val-Ala-Asp-Aldehyde AC-VAL-ALA-ASP-ALDEHYDE 25 mg
03AL01505 Ac-Val-Glu-Ile-Asp-Aldehyde AC-VAL-GLU-ILE-ASP-ALDEHYDE 5 mg
03AL01525 Ac-Val-Glu-Ile-Asp-Aldehyde AC-VAL-GLU-ILE-ASP-ALDEHYDE 25 mg
03AL04305 Caspase Inhibitor Caspase抑制劑 5 mg
03AL04505 Caspase 2/3 Inhibitor Caspase 2/3抑制劑 5 mg
03AL04405 Caspase 2 Inhibitor (Ich-1) Caspase 2抑制劑 5 mg
03AL04105 PKC Inhibitor / Caspase 3 Inhibitor PKC/ Caspase 3抑制劑 5 mg
03AL04205 Pro Caspase 3 Inhibitor Pro Caspase 3 抑制劑 5 mg
03OPH01001 Q-D(Ome)E(Ome)Vd(Ome)-Oph caspase3,7,10抑制劑 1 mg
03OPH01003 Q-D(Ome)E(Ome)Vd(Ome)-Oph caspase3,7,10抑制劑 3 mg
03OPH01005 Q-D(Ome)E(Ome)Vd(Ome)-Oph caspase3,7,10抑制劑 5 mg