上?，F(xiàn)貨皮質(zhì)醇（Cortisol）試劑盒

上?，F(xiàn)貨皮質(zhì)醇（Cortisol）試劑盒產(chǎn)品的用途：僅供科研ELISA檢測(cè)試劑盒價(jià)格：全國(guó)：歡迎選購(gòu)！,,到我司741653262，公司提供*進(jìn)口，國(guó)產(chǎn)elisa試劑盒，均為高性價(jià)比試劑盒檢測(cè)的原理：試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗(yàn)（ELISA）

上?，F(xiàn)貨皮質(zhì)醇（Cortisol）試劑盒一、高效、靈敏、特異的抗體；
二、穩(wěn)定的重復(fù)性和可靠性；
三、吸附性能好,空白值低,孔底透明度高的固相載體；
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型；
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟：
1)抗原表位的計(jì)算機(jī)篩選；
2)抗原的制備；
3)血清的采集；
4)間接ELISA方法的建立；
5)間接ELISA方法的敏感性和特異性驗(yàn)證；
6)試劑盒的穩(wěn)定性驗(yàn)證；
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清：操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿：EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液：1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿：將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存：如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
此IBL試劑盒能用于小鼠血清,EDTA血漿,細(xì)胞上清中產(chǎn)品定量檢測(cè)試劑盒成分
1預(yù)包被板:抗小鼠白介素-6兔子IgG,親合純化96T
2酶標(biāo)記抗體:(30倍濃縮)HRP標(biāo)記抗小鼠白介素-6兔子IgG,親合純化0.4mLx1
3標(biāo)準(zhǔn)品:重組小鼠白介素-60.5mLx2
4EIA緩沖液:含1%BSA,0.05%吐溫20BPS30mLx1
5標(biāo)記抗體稀釋液:含1%BSA,0.05%吐溫20BPS12mLx1
6顯色劑:TMB底物液15mLx1
7終止液:1N硫酸12mLx1
8濃縮洗滌液:(40倍濃縮)含1%BSA,0.05%吐溫20BPS50mLx1操作說明1實(shí)驗(yàn)所需器材(但試劑盒沒有提供)酶標(biāo)儀(450nm)微移液管及其吸嘴量筒及燒杯去離子水冰箱(4°C)坐標(biāo)紙(log/log)吸水紙?jiān)嚬?用于標(biāo)準(zhǔn)品稀釋)溫育箱(37°C±1°C)洗瓶(用于洗板)一次性試劑管(用于濃縮酶標(biāo)記抗體和顯色劑)

Motor - Plate lock, PyroQ96MD
Buffer AW1 (151 ml)
REPLI-g WTA Single Cell Kit (96)
Handscanner Touch TD1100
Transport box, flight case, EZ1xl
MagAttract Magnetic Rack
Transportation Handles, Qsym
QuantiTect SYBR Green PCR Kit(1000x50μl)    For 1000 x 50 ul reactions: 25 ml QuantiTect SYBR Green PCR Master Mix (providing a final concentration of 2.5 mM MgCl2), 20 ml RNase-free water
artus HBV RG PCR Kit (96) CE
artus Mycobac. diff. LC PCR Kit (24) CE
artus C.trachomatisPlus LC PCR Kit(24)CE
artus TPMT LC PCR Kit (24) CE
MAL Cartridge (5ml)
NeXtal Tubes JCSG Core II Suite
Valve, shutoff, QX
Safety valve, assy, QX
Lamp, UV, EZ1a
QX RNA Alignment Marker (1.5 ml)
Linear guide, Y axis, QX
Main switch ETA, 16 A
Control knob
WashHead, 8-fold
Barb Fitting, RCS1
Adapter, MagNa Pure LC 32-Well Plate
PAXgene Tissue DNA Kit (50)
Fan - rear, PyroQ96ID
Fan - peltier, PyroQ96ID
Fan - IPC 40x40, PyroQ96MD
Motor - X/Y, PyroQ96MD
Cable kit - X/Y, PyroQ96MD
Peltier module, PyroQ96
Mixer module, PyroQ96MD
Transportation box, SP, Qsym
Restore CD HP ProBook6570bWin7-32 Locked
PCR Buffer,10 x (1,2/2) without MgCl2
artus Parvo B19 TM PCR Kit (24) CE
QIAamp DNA FFPE Tissue Kit (50)
QuantiTect Rev.Transcription Kit (10)
Downtube, Reagent #1, AP98
Cable, assy, high voltage ON, QX
Piston, Anode (1), QX
Sensor, interlock1, with cable, QX
Key, Service, yellow, QX
QX DNA Size Marker 50 bp-1,5 kb (100 μL)
Shaker Rack Plugs (12)
Motor, y-drive (24V), BR8RD
MEL sensor flag, M48
PCB, Z plate breakout,8 ch., 8 core, QX1
Edge Finder Tool, DCU
Belt, Z-drive, pipettor, QIAC
Cover, end cap, Y-drive, QIAC
Enclosure, careHPV
DNeasy mericon 96 QIAcube HT Kit (5)
Supplemental Microbial qPCR MM (Rox)
Microbial DNA-Free Water
HiPerFect Transfection Reagent (1 ml)
Heating block assembly, BS96
Tip b holder, BS15
Cylinder hinge brass, BS15
Linear guide, BS15
Transportation box 1, LiquiChip
Transportation box , BioSprint 15
QuantiTect Rev.Transcription Kit (50)    "For 50 x 20 μl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.
Suitable only for cDNA synthesis followed by real-time analyses."
QuantiTect Primer Assay (200)    For 200 x 50 μl reactions: lyophilized mix of forward and reverse primers for real-time analysis with SYBR Green detection.
EZ1 Advanced DNA Blood Card
EZ1 Advanced Test Card
Card slot, EZ1a
EpiTect MethyLight Assay Hs-ONECUT2(100)    "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

For TaqMan probe-based real-time quantification of methylated or unmethylated CpG sites"
Investigator IDproof Server Key
AIV-H9 RT-PCR REAGENT LE
PG Influenza A RT-PCR Assay (48) LE Z
NDV Univ. RT-PCR REAGENT LE
Bottle, 500ml, wide mouth
Tool, thermistor calibration, RGx
mericon Quant RoundUp Ready Soy (48)
Cover, top, w display, QXP
Plate stacker, detachable    Unit for stacking up to ten 96-well plates; for use with the robotic handling system
Board CPU, EZ1a
SOW QIAsoft  MDx Dispens. RNase-free.PRO
Rotor-Gene Q MDx 6Plex no PC
PG AIV-H5 RT-PCR Kit (48) LE Z
PG AIV-H7 RT-PCR Kit (48) LE Z
Kit, upg-x/y-drive, portal, Qsym
mericon Mon 810 Corn (96)
mericon GMO Screen 35S-pat  (24)
Reagent holder, 1-trough 20 ml    Holder for acmodating a single disposable trough (20 ml) on the worktable; must be used with the reagent holder tray
Incubatorabdeckung Immucor (9194)
Service Kit Plato 7000 Complete
Verbindungsplaettchen Tischplatte P7
Twin Head (Tandem) Washer Pump (Vaccuum)
Syringe pump Board
Mikrowin Kinetic Software
Trough-Rack spezial
Sensor Distribution Board
Tubing, Valve to Pump