現(xiàn)貨產(chǎn)品絲裂原活化蛋白激酶激酶6(MKK6)試劑盒試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.
現(xiàn)貨產(chǎn)品絲裂原活化蛋白激酶激酶6(MKK6)試劑盒免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).
HBsAg試劑特點(檢測模式:臨床抗體夾心法):包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性(99.98%)提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說(PEI)HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml
ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP(辣根過氧化物酶)酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與*次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性.
Anti-CaMK2b(calcium/calmodulin-dependent protein kinase II beita) 鈣/鈣調(diào)素依賴蛋白激酶2b抗體
Anti-CAS (Cellular Apoptosis Susceptibility) 細胞凋亡敏感性基因
Anti-Caspase-1 (ICE/CASP-1/P45) 天冬氨酸-胱氨酸特異性蛋白酶家族抗體
Anti-Caspase-10 半胱胺酸蛋白酶-10抗體
Anti-Caspase-12 半胱胺酸蛋白酶蛋白-12抗體
Anti-Caspase-13 半胱胺酸蛋白酶蛋白-13抗體
Anti-Caspase-3/CPP32/SCA-1 半胱胺酸蛋白酶蛋白-3抗體
Anti-Caspase-6 (CT) 半胱胺酸蛋白酶蛋白-6抗體(C端)
Anti-Caspase-6 (NT) 半胱胺酸蛋白酶蛋白-6抗體(N端)
Anti-Caspase-8 (pro Mch5) 半胱氨酸蛋白酶8抗體
Anti-Caspase-9 白介素1-β轉(zhuǎn)化酶樣凋亡蛋白酶6抗體
Anti-Caspase-9 白介素1-β轉(zhuǎn)化酶樣凋亡蛋白酶6抗體
Anti-β-catenin/β-cats β-連環(huán)蛋白抗體
Anti-NAIF1 protein( CB12-327) 核凋亡誘導(dǎo)因子蛋白1
Anti-CBFA1/RUNX2 (Runt-related Transcription Factor 2) 成骨特異性轉(zhuǎn)錄因子抗體
Anti-CCK8(Cholecystokinin-8) 膽囊收縮素抗體/縮膽囊素抗體(八肽)
Anti-CCP (cyclic citrullinated peptide) 兔抗瓜氨酸環(huán)肽抗體
Anti-CCR-1(CC chemokine receptor 1) CC趨化因子受體1抗體
Anti-CCR-2/5(CC chemokine receptor 2/5) CC趨化因子受體2/5抗體
anti-CCR5/RANTES(regulated on activation normal T cell expressed and secreted) CC趨化因子受體5/依賴激活T細胞分泌調(diào)節(jié)蛋白
Anti-CCR-3(CC chemokine receptor 3) CC趨化因子受體3抗體
Anti-CCR-4(CC chemokine receptor 4) CC趨化因子受體4抗體
Anti-CCR-7(CC chemokine receptor 7) CC趨化因子受體7抗體
Anti-CD10(Mouse Anti-Human CD10 Monoclonal Antibody) 鼠抗人CD10單克隆抗體
Anti-CD10/CALLA (Neutral Endopeptidase) CD10抗體
Anti-CD105/Endoglin/TGF-β receptor CD105/轉(zhuǎn)化生長因子β受體抗體
Anti-CD117/c-kit/SCFR 干細胞生長因子受體/細胞表面分化抗原抗體
Anti-CD133 antigen 造血干細胞抗原CD133抗體
anti-Syndecan-1/CD138 多配體聚糖抗體
Anti-CD14(cluster of differentiation antigen 14) CD14抗體
anti-CD28 CD28抗體
Anti-CD144/VECD (Vascularendothelial cell cadherin/VE-cadherin) 上皮型鈣粘附分子/血管內(nèi)皮鈣粘蛋白抗體
Anti-CD147/EMMPRIN(Extracellular matrix metalloproteinase inducer) 單核-巨噬細胞基質(zhì)金屬蛋白酶抗體
anti-CD166/ALCAM(activated leukocyte celladhesion molecule) 活化白細胞粘附分子抗體
anti-ED-1 單核/巨噬細胞表面特異性標(biāo)志抗原抗體
Anti-CD167a/DDR1 CD167a抗體
Anti-CD169/Siglec-1/sialoadhesin(Sn)(Sialic acid-binding Ig-like lectin 1) CD169抗體
Anti-CD17 CD17抗體
Anti-CD18/LFA-1(Leukocyte Function Associated Antigen) 白細胞粘附蛋白抗體
Anti-CD184/CXC-R4(chemokine receptor type 4) 細胞表面趨化因子受體4抗體
Anti-CD19 (B-lymphocyte antigen CD19 precursor) CD19抗體
Anti-CD20 CD20抗體
Anti-CD24 CD24抗體
Phospho-IGF-IR (Y1161) Cell-Based Fluorometric ELISA Kit
Phospho-IGF-IR (Y1165/Y1166) Cell-Based Fluorometric ELISA Kit
Phospho-IGF-IR (Y1346) Cell-Based Fluorometric ELISA Kit
Phospho-IκB-α (Y42) Cell-Based Fluorometric ELISA Kit
Phospho-IKKβ (Y188) Cell-Based Fluorometric ELISA Kit
Phospho-IRAK-1 (T100) Cell-Based Fluorometric ELISA Kit
Phospho-Cytokeratin 8 (S432) Cell-Based Fluorometric ELISA Kit
Phospho-Cytokeratin 8 (S73) Cell-Based Fluorometric ELISA Kit
Phospho-LIMK-1 (T508) Cell-Based Fluorometric ELISA Kit
Phospho-mTOR (S2448) Cell-Based Fluorometric ELISA Kit
Phospho-mTOR (S2481) Cell-Based Fluorometric ELISA Kit
Phospho-mTOR (T2446) Cell-Based Fluorometric ELISA Kit
Phospho-c-Myc (T58) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p105 (S932) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (S276) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (S311) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (S468) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (S529) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (S536) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (T254) Cell-Based Fluorometric ELISA Kit
Phospho-NFκB-p65 (T435) Cell-Based Fluorometric ELISA Kit
Phospho-p21 (T145) Cell-Based Fluorometric ELISA Kit
Phospho-p53 (S15) Cell-Based Fluorometric ELISA Kit
Phospho-p53 (S315) Cell-Based Fluorometric ELISA Kit
Phospho-p53 (S366) Cell-Based Fluorometric ELISA Kit
Phospho-p53 (S6) Cell-Based Fluorometric ELISA Kit
Phospho-p53 (S9) Cell-Based Fluorometric ELISA Kit
Phospho-p63 (S395) Cell-Based Fluorometric ELISA Kit
Phospho-p73 (Y99) Cell-Based Fluorometric ELISA Kit
Phospho-RelB (S552) Cell-Based Fluorometric ELISA Kit
Phospho-SGK1 (S422) Cell-Based Fluorometric ELISA Kit
Phospho-SGK1 (S78) Cell-Based Fluorometric ELISA Kit
Phospho-Smad2 (S250) Cell-Based Fluorometric ELISA Kit
Phospho-Smad2 (S467) Cell-Based Fluorometric ELISA Kit
Phospho-Smad2 (T220) Cell-Based Fluorometric ELISA Kit
Phospho-Smad2/3 (T8) Cell-Based Fluorometric ELISA Kit
Phospho-Sp1 (T453) Cell-Based Fluorometric ELISA Kit
Phospho-Sp1 (T739) Cell-Based Fluorometric ELISA Kit
Phospho-c-Src (S75) Cell-Based Fluorometric ELISA Kit
Phospho-c-Src (Y216) Cell-Based Fluorometric ELISA Kit
Phospho-c-Src (Y419) Cell-Based Fluorometric ELISA Kit