皮質(zhì)醇檢測(cè)試劑盒(血清)齊一生物的ELISA試劑盒是您的*選擇:每個(gè)試劑盒都經(jīng)過嚴(yán)格的質(zhì)檢控制,包括:板內(nèi)、板間實(shí)驗(yàn)精度,靈敏度,回收率,穩(wěn)定性等指標(biāo)的測(cè)定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測(cè)體系簡(jiǎn)單而直接,可根據(jù)450 nm波長(zhǎng)的讀值迅速計(jì)算定量結(jié)果。試劑盒中提供的檢測(cè)抗體能夠識(shí)別全長(zhǎng)靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測(cè)結(jié)果更穩(wěn)定可靠
皮質(zhì)醇檢測(cè)試劑盒(血清)操作步驟:
1.標(biāo)準(zhǔn)品的稀釋與加樣:在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在*、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在*、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻;然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻;然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻;混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉。(稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L)。
2.加樣:分別設(shè)空白孔(空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、待測(cè)樣品孔。在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl(樣品zui終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻。
3.溫育:用封板膜封板后置37℃溫育30分鐘。
4.配液:將30(48T的20倍)倍濃縮洗滌液用蒸餾水30(48T的20倍)倍稀釋后備用。
5.洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
6.加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。
7.溫育:操作同3。
8.洗滌:操作同5。
9.顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
10.終止:每孔加終止液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。
11.測(cè)定:以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度(OD值)。 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。
(R型)原人參二醇
20(R)-人參皂苷RH2
原人參二醇30636-90-9
原人參三醇1453-93-6
25-甲氧基-原人參三醇
肉豆蔻木脂素171485-39-5
肉豆蔻酸544-63-8
染料木素446-72-0
染料木苷529-59-9
升麻素苷80681-45-4
升麻素37921-38-3
升麻酮醇-3-O-L-阿拉伯糖苷
圣草次苷13463-28-0
圣草酚552-58-9
蛇床子素484-12-8
8-O-乙酰山梔苷甲酯57420-46-9
山梔苷甲酯64421-28-9
山奈酚520-18-3
山奈苷482-38-2
山奈素491-54-3
水楊苷138-52-3
水楊酸甲酯119-36-8
水楊酸69-72-7
石杉?jí)A甲102518-79-6
石杉?jí)A乙(95%)103548-82-9
3,4,5-*氧基苯甲酸甲酯1916-07-0
3,4,5-*氧基苯甲酸118-41-2
石斛堿2115-91-5
水飛薊賓22888-70-6
異水飛薊賓72581-71-6
石吊蘭素152743-19-6
麝香酮541-91-3
酮麝香81-14-1
桑辛素62596-29-6
桑色素480-16-0
桑皮苷A102841-42-9
桑皮苷C102841-43-0
桑根酮 C 80651-76-9
桑根酮 D 81422-93-7
D-松醇10284-63-6
山姜素36052-37-6
水晶蘭苷5945-50-6
鼠李糖6155-35-7
穗花杉雙黃酮1617-53-4
斯皮諾素72063-39-9
酸棗仁皂苷A55466-04-1
酸棗仁皂苷B55466-05-2
酸棗仁皂苷A1194851-84-8
酸棗仁皂苷B168144-21-8
松脂醇二葡萄糖苷63902-38-5
桃葉珊瑚苷479-98-1
商陸皂苷甲65497-07-6
商陸皂苷元 1802-12-6
三葉豆紫檀苷6807-83-6
水仙苷604-80-8
沙苑子苷A146501-37-3
α-三聯(lián)噻吩1081-34-1
三白草酮177931-17-8
麝香草酚 89-83-8
三七皂苷R180418-24-2
三七皂苷Ft1
R-三七皂苷R2
三七總皂苷(UV95%)
三七素
1,3,5,8-四羥基山酮
山椒子烯酮 78804-17-8
山椒子烯醇 78804-17-8
山豆根色滿二氫黃酮Ⅰ
酸漿苦味素L
松蘿酸7562-61-0
松果菊苷82854-37-3
山柰酚-3-O-蕓香糖苷17650-84-9
山奈酚-7-葡萄糖苷16290-07-6
山奈酚-4’-葡萄糖苷52222-74-9
山奈酚-3-O-β-D-槐糖苷19895-95-5
天然冰片507-70-0
1-脫氧野尻霉素19130-96-2
特女貞苷39011-92-2
塔拉薩敏
土荊皮甲酸 82508-32-5
土荊皮乙酸82508-31-4
甜菜堿107-43-7
鹽酸甜菜堿590-46-5
通關(guān)藤苷H191729-45-0
褪黑素73-31-4
11-酮基乳香酸17019-92-0
甜菊醇471-80-7
藤黃酸(鑒別用) 2752-65-0
新藤黃酸
甜橙黃酮2306-27-6
太子參環(huán)肽B
苔黑酚葡萄糖苷(97%)
Waste box, blue, w yellow lid, Qsym 9021636 qiagen
Motor, vertical, replacement kit, RP 9238490 qiagen
Fitting Valve (5) 9238221 qiagen
Kerk Nut, Capper Up/Down, AP196/98 9240673 qiagen
Nut, Flangeless,1/4-28, Green, AP 9240667 qiagen
Nut, Flangeless,1/4-28, Red, AP 9240664 qiagen
MAC Valve, Manifold, Pressure, AP98 9240596 qiagen
QIAsoft 4.x, BR3000 Cust. Edition 9013864 qiagen
Cover, transparent, BS96 9021192 qiagen
Skin Panel, Upper Right Side, DCU 9021113 qiagen
Skin Panel, Upper Center Front, DCU 9021109 qiagen
Skin Panel, Lower Right Side, DCU 9021104 qiagen
Bracket, transfer module, Qsym 9021032 qiagen
Waste bag holder, AS, Qsym 9021025 qiagen
Multi.Carrier (2) for 12 cult.tubes 15ml 9020979 qiagen
Cable, HEPA filter, SMC, QGX 9020910 qiagen
Cable, mains harness, C34 9020714 qiagen
Panel, front, Cabinet Qsym 9020636 qiagen
Tool, LED spot size insert, RG 9020624 qiagen
PM kit, C12 & QIAgility (UV), CG1 9020319 qiagen
Motor, y-drive (24V), BR8RD 9017625 qiagen
LED, LightStrip (white), Qsym 9020254 qiagen
Holder, VacTrap, BR8 9017354 qiagen
PCB, lrge breakout, 12 core to rev8, CC2 9020201 qiagen
PCB, Z plate breakout,8 ch., 8 core, QX1 9020199 qiagen
PCB, vac pump, QXT 9020193 qiagen
Fitting,1/16MNPT,1/8, forWasteBottle(5) 9239523 qiagen
Assy,Terminated,RedLED,SDS (2) 9239536 qiagen
Fuse&Holder,1Amp,Fblo,PicoSmd(5) 9239529 qiagen
Mask, protection - chamber bottom, RG3 9020159 qiagen
QIAplex MDD HPV Genotyping LQ Software 9020145 qiagen
USB cable, QGX 9020114 qiagen
Packaging, TLyser2 9020102 qiagen
Shipping Lock Screw, TLyser 2 9020100 qiagen
Left Ratchet Holder, TLyser 2 9020094 qiagen
Motor 24V DC, TLyser 2 9020090 qiagen
Tubing. Peek, 1/6OD, 010ID, 10 (qty 5) 9239518 qiagen
Hyd Arm Optic Switch 9239492 qiagen
3A, 250V Fast Acting Fuse 9239449 qiagen
Sensor, pressure, 1600-1mbar 9232867 qiagen
Sealing ring, auto vac, SBS top, BR3000 9236775 qiagen
BR 8000, PM Service 9236509 qiagen
Z-Axis Coupling, EZ1 9239360 qiagen
BR 9604, Full Agreement 9236478 qiagen
#Agreement, 9604, 2PM, 1 yr 9236476 qiagen
Block, 2-way, v1.02 9232726 qiagen
Cable Assy Extender, 2 wire, 22AWG, 12in 9239540 qiagen
Upgr. LiquiChip Analyzer (RUO) 9239396 qiagen
EX XY Motor Driver w/Encoder 9239496 qiagen
Sheath Fluid Tubing w/DeBubbler 9239494 qiagen
EX Analog PCBA 9239487 qiagen
Centrifuge, PM Service 9236515 qiagen
Camera, bar-code, BL180, P3xxx/BR3000 9232888 qiagen
Cover, Wash Station, BR8 RD 9236636 qiagen
Travel Charge, additional, 1 day 9236451 qiagen
Fitting, pumping tube, black 9232725 qiagen
Label, BioRobot 9604 9232712 qiagen
Sensor, plate switch, TWI 9236429 qiagen
Installation, BR MDx DSP 9239142 qiagen
Dilutor, Kloehn Versa3 9239070 qiagen
Power Cord, USA 9232568 qiagen
Target Prep, Premium Agreement 9238970 qiagen
Angle rotor for 8 Falcon tubes 50 ml 9238791 qiagen
Cable, power PCON98 board, BR8000 9232328 qiagen
T piece, pressure relief valve, dispense 9232188 qiagen
Upper casing, BS96 9238906 qiagen
Elution adapter, auto vac, BR8000 9231863 qiagen
Service manual Zent3, german 9238681 qiagen
Grease, load-bearing bolts 9238680 qiagen
Spacer, DT-Adapter, BR8 9231466 qiagen
QIAVac Multiwell Base 9014582 qiagen
Reagent Chamber Lid, PyroQ24 9022195 qiagen
Sensor, Z-axis, with cable, QXA 9022022 qiagen
Kit, stickers, eluate drawer, Qsym 9021653 qiagen
Gateway 2, CC-Link, DCU 9021623 qiagen
Yellow grease for PM, EZ1 9021289 qiagen
Reservoir, 500ml w/cap, AP96/196 9240671 qiagen
Opto Sensor, Interlocks/Latch Lock, AP 9240640 qiagen
Opto Sensor, Common, AP 9240639 qiagen
Power Supply, International, AP97 9240633 qiagen
Fuse, 0.25A, FNM, AP96 9240614 qiagen
Fuse, 15.0A, FNM, AP96 9240613 qiagen
Filter Assembly, Pressure, AP196/98 9240585 qiagen
Cable, Vacuum Airflow Sensor, AP196/98 9240580 qiagen
Syringe, 2.5ml 9232772 qiagen