復(fù)制蛋白A(RPA-70)檢測試劑盒(血清.血漿.組織)齊一生物科技(上海)有限公司經(jīng)營試劑種類眾多:覆蓋分析化學(xué)品,無機(jī)化學(xué)品,有機(jī)化學(xué)品,生物化學(xué)品,材料化學(xué)品等.試劑信息齊全:包含試劑特性描述,詳細(xì)質(zhì)量信息,應(yīng)用方法,標(biāo)準(zhǔn),科研用途以及正在開發(fā)的前沿應(yīng)用歡迎前來選購齊一生物科技(上海)有限公司客服熱:4009910197/
復(fù)制蛋白A(RPA-70)檢測試劑盒(血清.血漿.組織)此IBL試劑盒能用于小鼠血清,EDTA血漿,細(xì)胞上清中產(chǎn)品定量檢測試劑盒成分
1預(yù)包被板:抗小鼠白介素-6兔子IgG,親合純化96T
2酶標(biāo)記抗體:(30倍濃縮)HRP標(biāo)記抗小鼠白介素-6兔子IgG,親合純化0.4mLx1
3標(biāo)準(zhǔn)品:重組小鼠白介素-60.5mLx2
4EIA緩沖液:含1%BSA,0.05%吐溫20BPS30mLx1
5標(biāo)記抗體稀釋液:含1%BSA,0.05%吐溫20BPS12mLx1
6顯色劑:TMB底物液15mLx1
7終止液:1N硫酸12mLx1
8濃縮洗滌液:(40倍濃縮)含1%BSA,0.05%吐溫20BPS50mLx1操作說明1實(shí)驗(yàn)所需器材(但試劑盒沒有提供)酶標(biāo)儀(450nm)微移液管及其吸嘴量筒及燒杯去離子水冰箱(4°C)坐標(biāo)紙(log/log)吸水紙?jiān)嚬?用于標(biāo)準(zhǔn)品稀釋)溫育箱(37°C±1°C)洗瓶(用于洗板)一次性試劑管(用于濃縮酶標(biāo)記抗體和顯色劑)
操作方法
雙抗體夾心法
1.包被:用0.05MPH9.牰碳酸鹽包被緩沖液將抗體稀釋蛋白質(zhì)含量為1~10μg/ml.在每個聚苯乙烯板的反應(yīng)孔中加0.1ml,4℃隔夜.次日,棄去孔內(nèi)溶液,用洗滌緩沖液洗3次,每次3分鐘.(簡稱洗滌,下同).
2. 加樣:加一定稀釋的待檢樣品0.1ml于上述已包被之反應(yīng)孔中,置37℃孵育1小時.然后洗滌.(同時做空白孔,陰性對照孔及陽性對照孔).
3. 加酶標(biāo)抗體:于各反應(yīng)孔中,加入新鮮稀釋的酶標(biāo)抗體(經(jīng)滴定后的稀釋度)0.1ml.37℃孵育0.5~1小時,洗滌.
4. 加底物液顯色:于各反應(yīng)孔中加入臨時配制的TMB底物溶液0.1ml,37℃10~30分鐘.
5. 終止反應(yīng):于各反應(yīng)孔中加入2M硫酸0.05ml.
6. 結(jié)果判定:可于白色背景上,直接用肉眼觀察結(jié)果:反應(yīng)孔內(nèi)顏色越深,陽性程度越強(qiáng),陰性反應(yīng)為無色或極淺,依據(jù)所呈顏色的深淺,以“+”、“-”號表示.也可測OD值:在ELISA檢測儀上,于450nm(若以ABTS顯色,則410nm)處,以空白對照孔調(diào)零后測各孔OD值,若大于規(guī)定的陰性對照OD值的2.1倍,即為陽性.
Anti-CK19(Cytokeratin 19) 細(xì)胞角蛋白19抗體
Anti-CK2(casein kinase 2) 酪氨酸激酶2抗體
Anti-CK4 (Cytokeratin 4) 細(xì)胞角蛋白4抗體
Anti-CK5 (Cytokeratin 5) 細(xì)胞角蛋白5抗體
Anti-CK8(Cytokeratin 8) 細(xì)胞角蛋白8抗體
anti-CKLFSF2(human)(chemokine-like factor superfamily 2) 人類新細(xì)胞因子/趨化素樣因子超家族成員2抗體
claudin-5 緊密連接蛋白-5抗體
CKIP-1(casein kinase 2 interaction protein 1) 酪蛋白激酶2相互作用蛋白1抗體
anti-Clostridium perfringens type D 兔抗D型產(chǎn)氣莢膜梭菌抗體
Anti-C-Mer/MERTK (proto-oncogene tyrosine kinase) c-mer原癌基因酪氨酸激酶抗體
Anti-C-Met/HGFR(Hepatocyte growth factor receptor) 肝細(xì)胞生長因子受體(原癌基因蛋白)抗體
Anti-C-Myc/MYC/MTLC 致癌基因抗體
anti-c-Myc tag c-Myc tag標(biāo)簽抗體
Anti-CNP/CNPase ( 2',3'-cyclic-nucleotide 3'-phosphodiesterase) C型鈉尿肽抗體
anti-CNTF(Ciliary Neurotrophic Factor) 睫狀神經(jīng)營養(yǎng)因子抗體
Anti-Collagen Ⅱ(Anti-Collagen Type Ⅱ) 抗Ⅱ型膠原抗體(Collagen Ⅱ Ⅱ型膠原)
Anti-Collagen Ⅲ(Anti-Collagen Type Ⅲ) 抗Ⅲ型膠原抗體(Collagen Ⅲ Ⅲ型膠原)
Anti-Collagen Ⅳ(Anti-Collagen Type Ⅳ) 抗IV型膠原抗體(Collagen Ⅳ Ⅳ 型膠原)
Anti-Collagen Ⅵ(Collagen Type Ⅵ) 抗Ⅵ型膠原抗體
Anti-Collagen I(Anti-Collagen Type I) 抗I型膠原抗體(I型膠原)
Anti-CollagenⅤ(Anti-Collagen Type Ⅴ) 抗Ⅴ型膠原抗體(CollagenⅤ Ⅴ型膠原)
Anti-CollagenⅩ(Collagen TypeⅩ) 抗Ⅹ型膠原抗體
Anti-COX-1 (Prostaglandin-endoperoxide synthase-1) 前列腺素氧化環(huán)化酶1抗體
Anti-COX-2 (Prostaglandin-endoperoxide synthase-2) 前列腺素氧化環(huán)化酶2抗體
Anti-C-PC(c-phycocyanin ) C-藻藍(lán)素/藻藍(lán)蛋白抗體
Anti-C-Peptide( C-PEP ) C-肽
Anti-CR (Calretinin) 鈣結(jié)合蛋白抗體
Anti-C-reactive protin CRP C-反應(yīng)蛋白抗體
Anti-C-CRP(Mouse Anti-Human reactive protin CRP Antibody) 鼠抗人C-反應(yīng)蛋白單克隆抗體
Anti-CREB-1(cAMP responsive element binding protein-1) 環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體
Anti-CREB-1(cAMP responsive element binding protein-1) 環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體
Anti-P-CREB-1(p-cAMP responsive element binding protein-1) 磷酸化環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體
Anti-CRF (Corticotropin-Releasing Factor) 促腎上腺皮質(zhì)激素釋放因子
Anti-CRF (Corticotropin-Releasing Factor) 促腎上腺皮質(zhì)激素釋放因子
anti-CSP (circumsporozoite protein/Plasmodium yoelii) 環(huán)子孢子蛋白(約氏瘧原蟲)抗體
Anti-CT (Calcitonin ) 降鈣素抗體
Anti-CT DNA (culf thymus DNA) 兔抗小牛胸腺DNA 抗體
Anti-CTGF (connective tissue growth factor) 結(jié)締組織生長因子抗體
Anti-CTLA-4/CD152(cytotoxic T-lymphocyte associated antigen-4) 淋巴細(xì)胞相關(guān)抗原4/細(xì)胞毒性T細(xì)胞抗原-4抗體
anti-CTn1 (cardiac troponin 1) 心肌肌鈣蛋白抗體
Anti-CT-R(Calcitonin receptor) 降鈣素受體抗體
Anti-CULLIN/Cdc53/CUL/SCFCdc4 CULLIN抗體
Anti-Cx40 (Connexin-40) 間隙連接蛋白40抗體
Anti-Cx43 (Connexin-43) 間隙連接蛋白43抗體
Anti-CX45 (Connexin-45) 間隙連接蛋白45抗體
Anti-CXC-R1 (CXC-chemokine receptor 1) 細(xì)胞表面趨化因子受體1抗體
Anti-CXC-R3/CD183 (Chemokine receptorC-X-C chemokine receptor type 3) 細(xì)胞表面趨化因子受體3抗體
anti-cx3cl1(chemokine (C-X3-C motif) ligand 1) 趨化因子(C-X3-C 基元)配體1抗體
Anti-CXorf36 (uncharacterized protein Cxorf36 precursor) 脫羧酶蛋白體3抗體
Anti-CXorf36 (uncharacterized protein Cxorf36 precursor) 脫羧酶蛋白體36抗體
Anti-Cyclin A 周期素A抗體
Anti-Cyclin B1 周期素B1抗體
Anti-Cyclin C 周期素C抗體
Anti-Cyclin D1 周期素D1抗體
Anti-Cyclin D2 周期素D2抗體
Anti-Cyclin E 周期素E/原癌基因抗體
Anti-Cyclin H 周期素H抗體
Anti-Cygb (cytoglobin) 細(xì)胞球蛋白抗體
Anti-CYP450(Cytochrome P450 monooxygenase) 細(xì)胞色素P450單氧化酶抗體
anti-Cyr61(cysteine rich 61) 富半胱氨酸肝素結(jié)合蛋白61
anti-CYP19 細(xì)胞色素P450 19抗體
Anti-Cytochrome C(Cyt C) 細(xì)胞色素 C抗體
PG AIV-H5 RT-PCR Assay (48) LE Z 1066845 qiagen
FMDV RT-PCR REAGENT LE 1066852 qiagen
Buffer MW2 (87ml) 1060993 qiagen
HiSpeed Plasmid Mega EF Kit (5) 1054578 qiagen
HCV RT-PCR REAGENT VI 1061091 qiagen
HSV PCR REAGENT II 1061095 qiagen
HCMV Quantification Standards 1061100 qiagen
TB PCR REAGENT I 1061102 qiagen
MP Quantification Standards 1061106 qiagen
Security Key, LC Policy Manager, encoded 9016732 qiagen
Lid, label BC1, RG3 9019887 qiagen
Cover, main, label CoResearch, RG3 9019884 qiagen
Optical source and detector assy , RG3 9019883 qiagen
Sensor, interlock1, with cable, QX 9019767 qiagen
Round Bucket w/ thread, sealable w/ cap 9238801 qiagen
Rotor-Disc Heat Sealer 230V 9019725 qiagen
PyroMark Vac Prep Tool, PyroQ96VW 9019663 qiagen
Motor, Z-axis, Cx2 9019618 qiagen
Pipettor, spring, Cx2 9019615 qiagen
O-ring, filter housing seal, 2mm, CX1 9019600 qiagen
Pulley, Idler X-axis, Cxx 9019572 qiagen
Stop pin, X-axis, Cxx 9019569 qiagen
Pipetting Station RD, RCS 9019536 qiagen
Home flag, Z-axis, CQx 9019534 qiagen
UV light assembly, QIA 9019531 qiagen
Motor, stepper, rotor drive, RG3 9019396 qiagen
Solenoid, cooling flap, RG6 9019352 qiagen
Fan, cooling blower, RG6 9019351 qiagen
Kit, Heater FET & drivers, RG6 9019334 qiagen
cable, flat band, 1500 mm 9234763 qiagen
Boundary, SIS, rigth 9234433 qiagen
AllStars Neg. siRNA AF 555 (5 nmol) 1027286 qiagen
Flange, pressure sensor 9233457 qiagen
Motor - X/Y, PyroQ24 9019231 qiagen
Pump - Pressure, PyroQ24 9019224 qiagen
Gripper arm kit, PyroQ96MD 9019219 qiagen
Barcode reader, PyroQ96MD 9019210 qiagen
Tip-disposal unit, v1.02 9011927 qiagen
PG AIV-H7 RT-PCR Assay (48) LE Z 1066849 qiagen
PG AIV-H9 RT-PCR Assay (48) LE Z 1066853 qiagen
Card, dispenser unit, BR8000 9232908 qiagen
Sample Preparation Reagents, 30ml Z 1061066 qiagen
HBV PCR REAGENT VI 1061122 qiagen
Plate Stacker Ambient Temp, RCS1 9016585 qiagen
Z-Rack, RCS1 9016567 qiagen
Transportation box , Twister I 9016534 qiagen
Transportation box 3, LiquiChip 9016532 qiagen
Transportation box 2, LiquiChip 9016531 qiagen
#App. Package, M48, Gene Expression 9016149 qiagen
Tubing, teflon, M6, 3 x 0.5 mm, 2.5 m 9011895 qiagen
Rect. Bucket Set (4), sealable with cap 9016514 qiagen
Maintenance Kit, Multiflow BR MDx V2 9016270 qiagen
MJR Microseal Pad for ALP 2296 MSP-1002 9016265 qiagen
Motor - Plate lock, PyroQ96MD 9019177 qiagen
Shaker adapter 8000, microplate 9011866 qiagen
Tubing Set, Pipet, Immucor 9011839 qiagen
Dilutor unit, BR8000 9011833 qiagen
Cable - X/Y, PyroQ96ID 9019136 qiagen
PCB 1 - Connection, PyroQ96ID 9019121 qiagen
PyroMark Vac Prep Tool, PyroQ24VW 9019064 qiagen
Board, motor driver, EZ1xl 9019018 qiagen
Door, metal frame, EZ1xl 9019001 qiagen
Adapter, Millipore Extraction Plate 9018944 qiagen
Magnet head for medium 96 plate, BS96 9016001 qiagen
Tube Holder, STS MDx, 9 mm (12) 9015970 qiagen
Fuse-2, 3.15A (for PSB board), M48 9015636 qiagen
EZ1 DNA TISS Card 9015588 qiagen
Lid, Cover, PlateReader 9015586 qiagen