血清血管緊張素Ⅰ(ANGⅠ)檢測試劑盒齊一生物科技有限公司產(chǎn)品的新老客戶,可享免費(fèi)代測實(shí)驗(yàn)服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報(bào)價(jià),可齊一生物科技有限公司.全國:歡迎選購!
血清血管緊張素Ⅰ(ANGⅠ)檢測試劑盒操作步驟
實(shí)驗(yàn)開始前,請?zhí)崆芭渲煤盟性噭?,試劑或樣品稀釋時(shí),均需混勻,混勻時(shí)盡量避免起泡。每次檢測都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí),用樣品稀釋液進(jìn)行稀釋,以使樣品符合試劑盒的檢測范圍。
1. 加樣:分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測樣品孔。空白孔加樣品稀釋液100μl,余孔分別加標(biāo)準(zhǔn)品或待測樣品100μl,注意不要有氣泡,加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻,酶標(biāo)板加上蓋或覆膜,37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性,每次實(shí)驗(yàn)請使用新的標(biāo)準(zhǔn)品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl(取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制),37℃,60分鐘。
3. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4. 每孔加辣根過氧化物酶標(biāo)記親和素工作液(同生物素標(biāo)記抗體工作液) 100μl,37℃,60分鐘。
5. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6. 依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內(nèi),此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色,后3-4孔梯度不明顯,即可終止)。
7. 依序每孔加終止溶液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性,底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8. 用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測。
PG AIV-H9 RT-PCR Assay (48) Z 1061107 qiagen
HIV-1 RT-PCR REAGENT I 1061112 qiagen
AIV-H7 RT-PCR REAGENT 1061114 qiagen
Vacuum Trap Bottle 4l, UTP 9016652 qiagen
Tubing Sys., Bottle, BR8 RD UTP 9016420 qiagen
Maintenance Kit, BR MDx v2 9016271 qiagen
Upgrade Kit, DISU, BR8000 9016268 qiagen
Bag for Waste Container 9016237 qiagen
Maintenance Kit, RoboVac, BR8000 I,SBS 9016122 qiagen
Window, PyroQ96MD 9019176 qiagen
Set, Tubing, PeristalticPump, In/Out, P3 9011840 qiagen
Reagent chamber lid, PyroQ96ID 9019137 qiagen
PCB 5 - Connection, PyroQ96ID 9019120 qiagen
PCB - Interconnection, PyroQ96ID 9019111 qiagen
PyroMark Q96 ID Software (5) 9019084 qiagen
PyroMark Q24 MDx Software 9019063 qiagen
Coupling, Z axis, EZ1xl 9019020 qiagen
Coil, EZ1xl 9019019 qiagen
Door lock assembly, EZ1xl 9019005 qiagen
Enclosure, Silver, DML3000 9022710 qiagen
Maintenance Kit, RoboVac, BR8000 II 9016069 qiagen
Modules, DISU, BR8000 v3.0 9016040 qiagen
Magnet head for PCR plateMagnet head for 9016000 qiagen
Z-drive unit, BR8000 v1.1 9015964 qiagen
Fuse-1, 6.3A (for PSB board), M48 9015635 qiagen
EZ1 DNA Buccal Swab Card 9015589 qiagen
EZ1 DNA BLD Card 9015585 qiagen
Pulley Overhead, BR8, RD v1.1 9015548 qiagen
Liner, worktable 9015539 qiagen
Cover, front, type C 9015535 qiagen
Power supply unit, plete 9015530 qiagen
Full Agreement Depot Repair, PC, 1 yr 9241261 qiagen
PCB 5 - Connection, PyroQ96MD 9019158 qiagen
Upgrade kit, LabHandT-RapidPlate 9014184 qiagen
ZLV Z-Modul; Washable Probe 0,9mm;BR8000 9014183 qiagen
Adapter, 96-well MP, BS96 9021190 qiagen
Skin Panel, PC Mount Switch Mount, DCU 9021107 qiagen
PLC Module, MES, DCU 9021084 qiagen
Shaker Assembly, DCU 9020992 qiagen
Lid, QXT 9020926 qiagen
Slideout electrical tray, SMC, QGY 9020923 qiagen
EZ1 DNA BACT Card 9016362 qiagen
Plate holder, long 9011926 qiagen
Elution Microtube Adapter, SBS 9236778 qiagen
IPC module, excl. HDD, PyroQ96ID 9019140 qiagen
Damper - X/Y table, PyroQ96ID 9019131 qiagen
IPC bus backplane, PyroQ96 9019109 qiagen
PyroMark IdentiFire SW 1.0 (5) 9019088 qiagen
Worktable, front bar, EZ1xl 9019023 qiagen
Heating block, EZ1xl 9019014 qiagen
Adapter, Tip Rack Holder for 96-Tip Rack 9018949 qiagen
Worktable, TargetPrep Layout 9016103 qiagen
Maintenance Kit, Basic, BR8000 II 9016068 qiagen
Maintenance Kit, RoboVac, BR8000 I 9016067 qiagen
Maintenance Kit, MultiFlow, BR8000 9016066 qiagen
Y-Motor, DC-Gear, AT3/B10 9016013 qiagen
SamTrak, Complete, BR8 RD v2.0 9015984 qiagen
Tube Holder, STS MDx, 12 mm (12) 9015971 qiagen
PCB - Interconnection, PyroQ96MD 9019151 qiagen
Connecting plate, BR3000-RapidPlate 9014185 qiagen
Latch Keeper, DCU 9021145 qiagen
Skin Panel, Upper Right Rear, DCU 9021112 qiagen
Tip Disposal Bag, DCU 9021095 qiagen
Cable, X Drive, DCU 9021004 qiagen
Packaging (plete), SMC, QGY 9020924 qiagen
PCB, UV, C34 9020718 qiagen
Universal block, C34 9020715 qiagen
LED assembly, C1x 9020705 qiagen
Firmware - Controller PCB, PyroQ96ID 9020692 qiagen
Cover - Top, PyroQ24 9020689 qiagen
PCB, transfer module, AS, Qsym 9020395 qiagen
Eluate in drawer, left, AS, Qsym 9020363 qiagen
AUTOPURE Waste Container 9017686 qiagen
Sample Carrier, plate, Qsym 9017660 qiagen
USB-A-B port, Qsym 9020255 qiagen
Slideout electrical tray (110 V), C1x 9020238 qiagen
Slideout electrical tray, SMPS, 12Q 9020230 qiagen
PCB, lrge breakout, non LLS, CC2 9020208 qiagen
Clamp, worktable (marked "I"), CX1 9020196 qiagen
Gasket, centrifuge lock, QIAC 9020185 qiagen
Handwheel, TLyser 2 9020097 qiagen
29201 NeutrAvidin Agarose 10 mL
29202 High Capacity NeutrAvidin Agarose 5 mL
29204 High Capacity NeutrAvidin Agarose 10 mL
29339 Alkaline Phosphatase, Biotinylated 1 mg
29570 Vacuum Hydrolysis Tube, 1mL 1 Tube
29571 Vacuum Hydrolysis Tube, 6mL 1 Tube
29572 Vacuum Hydrolysis Tube, 18mL 1 Tube
29700 Urea, Sequanal Grade 1000 GM
29810 Ethylene Glycol 200 mL
29920 Disposable Columns, 2mL 100/PKG
29922 Disposable Columns, 5mL 100/PK
29923 Disposable Column Funnels 50/PK
29924 Disposable Columns, 10mL 100/PKG
29925 Disposable Column Trial Pack Kit
29986 EZ-Link Psoralen-PEG3-Biotin 5 mg
29988 Rebinant Protein G, Biotinylated 0.5 mg
29989 Protein A, Biotinylated 1 mg
29994 Pierce Avidin, Horseradish Peroxidase Conjugated 5 mg
29997 Rebinant Protein L, Biotinylated 0.5 mg
30030 Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM) 500 mL
31000 NeutrAvidin Protein 10 mg
31001 NeutrAvidin Protein, Horseradish Peroxidase Conjμgated 2 mg
31002 NeutrAvidin Protein, Alkaline Phosphatase Conjμgated 2 mg
31006 NeutrAvidin Protein, Fluorescein Conjugated 5 MG
31007 Maleimide-Activated NeutrAvidin Protein 5 mg
31030 High Sensitivity NeutrAvidin-HRP 0.5 mL
31032 High Sensitivity NeutrAvidin-HRP 5 mL
31050 NeutrAvidin Protein 100 mg
31055 NeutrAvidin Protein 1000 mg
31104 Pierce(R) Rabbit Anti-Chicken IgY (H+L) 2.0 MG
31105 Pierce(R) Rabbit Anti-Goat IgG (H+L), 2 MG 2 MG
31107 Pierce(R) Mouse Anti-Goat IgG (H+L) (min x HnMsRb Sr Prot) 1.5 MG
31109 Pierce(R) F(ab')2 Fragment Rabbit Anti-Goat IgG (H+L) (mi 0.5 MG
31111 Immunodiffusion Plates, Agarose Gelling Agent, Multiple Pattern 6/PKG
31115 Pierce(R) Goat Anti-Hamster IgG (H+L) 1.5 MG
31118 Pierce(R) Goat Anti-Human IgG (Gamma) 0.5 MG
31119 Pierce(R) Goat Anti-Human IgG (H+L), (min x BvHsMs Sr 1.5 MG
31122 Pierce(R) Goat Anti-Human IgG {F(ab')2} 2.0 MG
31124 Pierce(R) Goat Anti-Human IgM (u) 0.5 MG
31125 ImmunoPure Goat Anti-Human IgG (Fc, (min x BvHsMs Sr Prot 1 MG
31128 Pierce(R) Goat Anti-Human IgA + IgG + IgM (H+L) 2.0 MG
31129 Pierce(R) Goat Anti-Human Kappa Chain 0.5 MG
31130 Pierce(R) Goat Anti-Human IgG (H+L), 2 mg 2 MG
31131 Pierce(R) Goat Anti-Human Lambda Chain 0.5 MG
31132 Pierce(R) Goat Anti-Human IgG F(ab')2, (min X Bv Hs Ms Sr P 1.5 MG
31133 Pierce(R) Rabbit Anti-Goat IgG (Fc) 2.0 MG
31135 Pierce(R) Mouse Anti-Human IgG (H+L), (min x Ms Sr Prot) 2.0 MG
31136 Pierce(R) GOAT ANTI-HUMAN IgM Fc5u, 2 MG 2 MG
31137 Pierce(R) Mouse Anti-Human IgG (H+L), (min x BvHsMs Sr 1.5 MG
31140 Pierce(R) Goat Anti-Human Serum IgAa, 2 MG 2 MG
31142 Pierce(R) Rabbit Anti-Human IgG Fc, 2 MG 2 MG
31143 Pierce(R) Rabbit Anti-Human IgG (H+L) 2.0 MG
31146 Pierce(R) Human IgM (Myeloma), Whole Molecule, 2.0 mg 2 MG
31148 Pierce(R) Human IgA Serum, 2.0 mg 2 MG
31154 Pierce(R) Human IgG, Whole Molecule, 10 mg. 10 MG
31160 Pierce(R) Goat Anti-Mouse IgG (H+L), 2 mg 2 MG
31163 Pierce(R) F(ab')2 Fragment Goat Anti-Human IgG (Fc) 1.0 MG
31164 Pierce(R) GOAT ANTI-MOUSE IgG (H+L)(min X HnBvHs Sr PROT), 1.5 MG
31165 Pierce(R) F(ab')2 Fragment Goat Anti-Human IgG (H+L) 1.0 MG
31166 Pierce(R) Goat Anti-Mouse IgG F(ab')2, 2 mg 2 MG
31168 Pierce(R) Goat Anti-Mouse IgG Fc, 2 mg 2 MG
31170 Pierce(R) Goat Anti-Mouse IgG Fc (min X HnBvHs Sr PROT), 1.5 1.5 MG
31172 Pierce(R) GOAT ANTI-MOUSE IgM u, 2 MG 2 MG
31178 Pierce(R) F(ab')2 FRAGMENT GO AT ANTI-MOUSE IgMu, 1 MG 1 MG
31181 Pierce(R) Horse Anti-Mouse Ig G (H+L) 1.5 MG
31182 Pierce(R) GOAT ANTI-MOUSE IgG +IgM (H+L), 2 MG 2 MG
31185 Pierce(R) F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) 1.0 MG
31186 Pierce(R) F(ab')2 Fragment Goat Anti-Mouse IgM (u) 1.0 MG
31188 Pierce(R) Rabbit Anti-Mouse, IgG (H+L), 2 mg 2 MG
31190 Pierce(R) Rabbit Anti-Mouse IgG(H+L)(min X Hn Sr PROT), 1.5 MG
31192 Pierce(R) Rabbit Anti-Mouse IgG F(ab')2, 2 MG 2 MG
31194 Pierce(R) Rabbit Anti-Mouse IgG Fc, 2 mg 2 MG
31196 Pierce(R) Rabbit Anti-Mouse IgMu, 2 MG 2 MG
31198 Pierce(R) Rabbit Anti-Mouse IgG+IgM (H+L), 2 MG 2 MG
CRL-2266 SH-SY5Y(神經(jīng)母細(xì)胞瘤細(xì)胞) ATCC 株
CCL-222 COLO 205(結(jié)腸癌細(xì)胞) ATCC 株
CRL-1999 T/G HA-VSMC(人主動脈血管平滑肌細(xì)胞) ATCC 株
CRL-1730 HUVEC(人臍靜脈血管內(nèi)皮細(xì)胞) ATCC 株
CRL-1476 A10(齊一生物供應(yīng)主動脈血管平滑肌細(xì)胞) ATCC 株
CL-173 3T3-L1(小鼠脂肪細(xì)胞) ATCC 株
CRL-1811 A9(皮下結(jié)締組織細(xì)胞) ATCC 株
TIB-54 WEHI 22.1(B淋巴細(xì)胞 ) ATCC 株
CRL-1658 NIH/3T3(胚胎成纖維細(xì)胞) ATCC 株
CRL-2594 MC3T3-E1 Subclone 14(小鼠原成骨細(xì)胞) ATCC 株
CRL-1458 L6(齊一生物供應(yīng)成肌細(xì)胞 ) ATCC 株
CRL-1446 H9c2(2-1)(齊一生物供應(yīng)心肌細(xì)胞) ATCC 株
CRL-2765 RSC96(齊一生物供應(yīng)雪旺細(xì)胞) ATCC 株
CRL-9096 CHO/dhFr-(中國倉鼠二氫葉酸還原酶缺陷細(xì)胞) ATCC 株
CRL-1735 Lec1(卵巢細(xì)胞) ATCC 株
CRL-11268 293T/17 [HEK 293T/17] (人胚腎細(xì)胞) ATCC 株
AAV-293( 胚腎細(xì)胞 ) ATCC 株
CRL-11372 hFOB 1.19(SV40 轉(zhuǎn)染成骨細(xì)胞) ATCC 株
CRL-2122 CCD-1095Sk(皮膚細(xì)胞 ) ATCC 株
HTB-125 Hs 578Bst(乳腺細(xì)胞) ATCC 株
CRL-2407 NK-92(人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞) ATCC 株
CRL-2408 NK-92MI(人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞) ATCC 株
CRL-1993 HMy2.CIR(人B 淋巴母細(xì)胞) ATCC 株
CRL-1840 OK(負(fù)鼠(opossum)腎細(xì)胞) ATCC 株
CRL-1646 FO(骨髓瘤細(xì)胞) ATCC 株
TIB-71 RAW 264.7(單核巨噬細(xì)胞白血病) ATCC 株
CRL-1825 P19(畸胎癌細(xì)胞) ATCC 株
CCL-107 C6(膠質(zhì)瘤細(xì)胞) ATCC 株
CRL-2256 RBL-2H3(白血病細(xì)胞) ATCC 株
HTB-35 SiHa(子宮頸癌細(xì)胞) ATCC 株
CRL-1427 MG-63(骨肉瘤細(xì)胞) ATCC 株
HTB-113 HEC-1-B(子宮內(nèi)膜腺癌細(xì)胞) ATCC 株
HTB-94 SW1353(骨肉瘤細(xì)胞) ATCC 株
CRL-1978 ES-2(卵巢透明細(xì)胞癌細(xì)胞) ATCC 株
HTB-85 Saos-2(成骨肉瘤細(xì)胞) ATCC 株
HTB-132 MDA-MB-468(乳腺癌細(xì)胞) ATCC 株
HTB-58 SK-MES-1(肺鱗癌細(xì)胞) ATCC 株
HTB-129 MDA-MB-435S(乳腺癌細(xì)胞) ATCC 株
HTB-183 NCI-H661(大細(xì)胞肺癌細(xì)胞) ATCC 株
CRL-1500 ZR-75-1(乳腺癌細(xì)胞) ATCC 株
CRL-1848 NCI-H292(肺癌細(xì)胞(淋巴結(jié)轉(zhuǎn)移)) ATCC 株
HTB-126 Hs 578T(乳腺癌細(xì)胞) ATCC 株
CRL-1593.2 U-937(組織細(xì)胞淋巴瘤細(xì)胞) ATCC 株
CRL-1918 CFPAC-1(胰腺癌細(xì)胞) ATCC 株
CRL-2570 A3(淋巴細(xì)胞白血病細(xì)胞) ATCC 株
CRL-1469 PANC-1(胰腺癌細(xì)胞) ATCC 株
TIB-152 Jurkat,Clone E6-1(T 淋巴細(xì)胞白血病細(xì)胞) ATCC 株
HTB-52 SK-HEP-1(肝癌細(xì)胞) ATCC 株
CCL-240 HL-60(急性粒細(xì)胞白血病細(xì)胞) ATCC 株
HCCLM3(肝癌細(xì)胞) ATCC 株
CRL-1803 TT(人甲狀腺癌細(xì)胞) ATCC 株
CRL-8024 PLC/PRF/5(肝癌亞力山大細(xì)胞) ATCC 株
HTB-107 SW579(人甲狀腺癌細(xì)胞) ATCC 株
CRL-2577 RKO(結(jié)腸腺癌細(xì)胞) ATCC 株
HTB-43 FaDu(人咽鱗癌細(xì)胞) ATCC 株