甲氧芐啶農(nóng)殘檢測(cè)試劑盒詳細(xì)說明書請(qǐng)來電,或者在線客服進(jìn)行索要!齊一生物長期經(jīng)營ELISA試劑盒及抗體(免費(fèi)代測(cè))、分子學(xué)生物試劑盒、比色法,IFCC推薦法,化學(xué)氧化法,速率法等檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。誠信經(jīng)營,價(jià)格實(shí)惠,服務(wù)周到,質(zhì)量保障!提供各種實(shí)驗(yàn)代測(cè)服務(wù)。歡迎新老客戶!
甲氧芐啶農(nóng)殘檢測(cè)試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1:1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的生物素標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長處測(cè)定各孔的OD值.
9020580 Cable, DB9 M/F (UL) 6ft, DML 3000
9020588 Pulley, belt, DML 3000
9021016 Robot Stage, X Axis, VR, DCU
9013376 Maintenance Kit BR 8000 Research
9013279 BGR Upgrade-Kit Scanner PK80
9013272 Peristaltic Pump, RCS1
9013190 Motor, rotor, buffer track, BR8000
132772 NeXtal Stock Jeffamine M-600 (200) 100% v/v O-(2-Aminopropyl)-O'-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)
132620 NeXtal Stock Tricine (50) 1M Tricine - 50mL (4 x 12,5 ml tube)
132560 NeXtal Stock Magnesium chloride (50) 2M Magnesium chloride hexahydrate - 50mL (4 x 12,5 ml tube)
800171 Easy-Read Tubes, w/o prep "Sequencing of plasmid DNA and PCR products
DNA quality control not included
No repetition of sequencing
Data collected to maximal read length"
132546 NeXtal Stock Ferric chloride (50) 1M Ferric Chloride Hexahydrate - 50mL (4 x 12,5 ml tube)
132008 EasyXtal 15-Well Tool X-Seal (20) "20 empty 15-well crystallization plates with X-Seal crystallization supports
For manual setup of hanging drop crystallization experiments."
203645 HotStarTaq Plus Master Mix Kit (1000) "For 1000 x 20 μl reactions: 12 x 0.85 ml HotStarTaq Plus Master Mix, containing 1000 units of HotStarTaq Plus DNA Polymerase total, 4 x 0.55 ml CoralLoad Concentrate, 8 x 1.9 ml RNase-Free Water
HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, and performance as HotStarTaq Plus DNA Polymerase NOW in a master mix format, which offers even faster results and added convenience."
203603 HotStarTaq Plus DNA Polymerase (250) 1 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
203601 HotStarTaq Plus DNA Polymerase (50) Non published.
201912 dNTP Set, PCR Grade 100mM, (4x100μl) dNTP Set is a plete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 1000 x 50ul PCR reactions
201223 Taq PCR Core Kit (250) "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
180840 GeneRead qPCR SYBR Green/ROX MM (2)
180830 GeneRead qPCR SYBR Green/Fluor MM (2)
158767 Gentra Puregene Cell Kit (8 x 108)
34650 RGS·His Antibody, BSA-free (100 μg) 100 ug mouse anti-RGS(H)4 (lyophilized, BSA-free, for 1000 ml working solution)
32582 EasyXpress Disulfide Insect 10 rxns Kit
32149 QIAexpress Type IV Kit 5 ug each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose. pQE-40
31014 Ni-NTA Spin Columns (50) 50 Ni-NTA Spin Columns, Collection Tubes
30600 Ni-NTA Fast Start Kit (6)
9021350 Piston, connecting rod, TLyser LT
9022027 Sensor, Y-axis, with cable, QXA
9002142 careHPV test Controller
286115 cador N. caninum PCR Reagent (96)
282603 virotype Influenza A RT-PCR Kit (24)
282607 virotype Influenza A RT-PCR Kit (480)
280373 virotype BVDV RT-PCR Kit (24)
Dimethyl carbonate 碳酸二甲酯 [616-38-6]
5,5-Dimethyl-1,3-cyclohexanedione 5,5-二甲基-1,3-環(huán)己二酮 [126-81-8]
N’N-Dimethylformamide [DMF] N’,N-二甲基甲酰胺 [68-12-2]
N’N-Dimethylformamide [DMF] N’,N-二甲基甲酰胺 [68-12-2]
N’N-Dimethylformamide [DMF] N’,N-二甲基甲酰胺 [68-12-2]
N-N-Dimethylglycine hydrochloride N’,N-二甲基甘氨酸鹽酸鹽 [2491-06-7]
N-N-Dimethylglycine hydrochloride N’,N-二甲基甘氨酸鹽酸鹽 [2491-06-7]
Dimethylglyoxime 鎳試劑 [95-45-4]
Dimethylglyoxime sodium salt 二甲基甲酮 鈉鹽 [75006-64-3]
Dimethyl isophthalate 間苯二甲酸二甲酯 [1459-93-4]
Dimethyl maleate 馬來酸二甲酯 [624-48-6]
2,4-Dimethylnitrobenzene 2,4-二甲基硝基苯 [89-87-2]
2,6-Dimethylnitrobenzene 2,6-二甲基硝基苯 [81-20-9]
Dimethyl oxalate 草酸二甲酯 [553-90-2]
2,4-Dimethylphenol 2,4-二甲酚 [105-67-9]
2,6-Dimethylphenol 2,6-二甲酚 [576-26-1]
3,4-Dimethylpheno l 3,4-二甲酚 [95-65-8]
Dimethyl phthalate 鄰酞酸二甲酯 [131-11-3]
2,2-Dimethyl-1,3-propanediol 新戊二醇 [126-30-7]
Dimethyl succinate 丁二酸二甲酯 [106-65-0]
2,2-Dimethylsuccinic acid 2,2-二甲基琥珀酸 [597-43-3]
Dimethyl sulfone 甲基砜 [67-71-0]
Dimethyl sulfoxide (DMSO) 二甲亞砜 [67-68-5]
Dimethyl sulfoxide (DMSO) 二甲亞砜 [67-68-5]
Dimethyl sulfoxide (DMSO) 二甲亞砜 [67-68-5]
Dimethyl terephthalate 對(duì)苯二甲酸二甲酯 [120-61-6]
N,N’-Dimethylurea 二甲基脲 [96-31-1]
N,N’-Dimethylurea 二甲基脲 [96-31-1]
Dimethyl yellow 二甲基黃 [60-11-7]
Dimethyl yellow 二甲基黃 [60-11-7]
Dimetridazole 二甲硝咪唑 [551-92-8]
2,4-Dinitrobenzaldehyde 2,4-二硝基苯甲醛 [528-75-6]
1,3-Dinitrobenzene 1,3二 硝 基 苯 [99-65-0]
豬Ⅲ型前膠原氨基端肽(PⅢNT)檢測(cè)試劑盒(血清.血漿.組織)
豬低分子肝素(LMWH)檢測(cè)試劑盒(血清.血漿.組織)
豬單核細(xì)胞增多性李斯特菌素O((LLO)檢測(cè)試劑盒(血清.血漿.組織)
豬圓環(huán)病毒(PCV)檢測(cè)試劑盒(血清.血漿.組織)
豬圓環(huán)病毒抗體(PCV-Ab)檢測(cè)試劑盒(血清.血漿.組織)
豬氣喘病病毒抗體檢測(cè)試劑盒(血清.血漿.組織)
豬分泌型免疫球蛋白A(sIgA)檢測(cè)試劑盒(血清.血漿.組織)
豬辛型肝炎病毒((TTV-Ab)檢測(cè)試劑盒(血清.血漿.組織)
豬IgA 檢測(cè)試劑盒(血清.血漿.組織)
豬傳染性胃腸炎抗原(TGE-Ag)檢測(cè)試劑盒(血清.血漿.組織)
豬流行性腹瀉抗體(PEDAb)檢測(cè)試劑盒(血清.血漿.組織)
豬流行性腹瀉抗原(PED-Ag)檢測(cè)試劑盒(血清.血漿.組織)
豬傳染性胸膜肺炎抗原(PCP-Ag)檢測(cè)試劑盒(血清.血漿.組織)
豬瘟抗體(CSF-Ab)檢測(cè)試劑盒(血清.血漿.組織)
豬神經(jīng)生長因子(NGF)檢測(cè)試劑盒(血清.血漿.組織)
豬膠質(zhì)細(xì)胞系來源的神經(jīng)營養(yǎng)因子(GDNF)檢測(cè)試劑盒(血清.血漿.組織)
豬傳染性胸膜肺炎(PCP)檢測(cè)試劑盒(血清.血漿.組織)
豬副嗜血桿菌(Hps)檢測(cè)試劑盒(血清.血漿.組織)
豬鏈球菌(streptococcus)檢測(cè)試劑盒(血清.血漿.組織)
豬弓形蟲抗原(Tox-Ab)檢測(cè)試劑盒(血清.血漿.組織)
豬弓形蟲抗體(Tox-Ag)檢測(cè)試劑盒(血清.血漿.組織)
豬布魯氏菌(Brucella)檢測(cè)試劑盒(血清.血漿.組織)