Cyanine5.5 hydrazide,酰肼紅色熒光染料
產(chǎn)品介紹
Cyanine5.5 hydrazide
Cyanine5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides uickly react with carbonyl pounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates.
Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium periodate and labeled with Cyanine5.5 hydrazide to form Cyanine5.5 labeled antibodies. Since sugar residues in antibodies are located far from variable, epitope-binding site, this method is particularly good for the labeling of antibodies.
Cyanine5.5 is a near infrared fluorophore, an analog of Cy5.5®. It is particularly suitable for the applications which reuire low fluorescence background. The absorption and emission wavelength of the dye exhibit significant tissue penetration, so Cyanine5.5 is suitable for in vivo NIR imaging applications.
General properties
Appearance: | dark blue powder |
Molecular weight: | 669.73 |
Molecular formula: | C40H46Cl2N4O |
Solubility: | moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols) |
uality con*rol: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 684 |
ε, L⋅mol−1⋅cm−1: | 209000 |
Emission maximum, nm: | 710 |
Fluorescence uantum yield: | 0.2 |
CF260: | 0.07 |
CF280: | 0.03 |
結(jié)構(gòu)式：
Cyanine5.5 hydrazide,酰肼紅色熒光染料
西安凱新生物科技有限公司
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