Pimonidazole (Synonyms: 哌莫硝唑)
Pimonidazole 是一種缺氧標記物，用于瘤內(nèi)缺氧和細胞增殖的補充性研究。Pimonidazole 通過與大分子的共價結(jié)合或通過被還原形成還原性代謝物后在缺氧細胞中積累，可用于定性和定量評估腫L缺氧。
生物活性
Pimonidazole is a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in tumor[1]. Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after reduction of its nitro group, it can be used for qualitative and quantitative assessment of tumor hypoxia [2].
體外研究(In Vitro)
Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%.
Detection: Hypoxic cells were recognized by immunohistochemical detection of pimonidazole using a mouse monoclonal antibody. Cell proliferation was detected with a commercially available monoclonal antibody for proliferating cell nuclear antigen (PCNA). Assessment of hypoxia and cell proliferation was made qualitatively with light microscopy and quantitatively using point counting and image analysis software methods.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT02414048 | Marius Gustav Bredell|University of Zurich | April 2015 | Phase 2 | |
| NCT03718650 | H. Lee Moffitt Cancer Center and Research Institute|Varian Medical Systems | April 1, 2021 | Early Phase 1 | |
| NCT01248637 | University Health Network, Toronto | October 2010 |
分子量：254.29
性狀：Solid
Formula：C11H18N4O3
CAS 號：70132-50-2
中文名稱：哌莫硝唑； 乏氧示蹤劑
運輸條件：Room temperature in continental US; may vary elsewhere.
儲存方式
| Powder | -20°C | 3 years |
|---|---|---|
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
H2O : 1 mg/mL (3.93 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.9325 mL | 19.6626 mL | 39.3252 mL |
| 5 mM | --- | --- | --- |
| 10 mM | --- | --- | --- |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲存時，請在 6 個月內(nèi)使用，-20°C 儲存時，請在 1 個月內(nèi)使用。
參考文獻
[1]. Varia MA, et al. Pimonidazole: a novel hypoxia marker for complementary study of tumor hypoxia and cell proliferation in cervical carcinoma. Gynecol Oncol. 1998 Nov;71(2):270-7. [Content Brief]
[2]. Masaki Y, et al. Imaging Mass Spectrometry Revealed the Accumulation Characteristics of the 2-Nitroimidazole-Based Agent "Pimonidazole" in Hypoxia. PLoS One. 2016 Aug 31;11(8):e0161639. [Content Brief]
注：產(chǎn)品僅用于科研
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