Dickkopf相關(guān)蛋白1(DKK1)單克隆抗體
Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1)
SK; Dickkopf Homolog 1(Xenopus Laevis)
編號MAA741Hu22
物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
來源單抗制備
宿主小鼠
效價(克隆號)C5
Ig亞型IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原RPA741Hu01-Dickkopf相關(guān)蛋白1(DKK1)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液(pH7.4,containing 0.05% Proclin-300,50% glycerol)
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù),請參見流式抗體。下載英文說明書 中文說明書
規(guī)格20µl100µl 200µl 1ml 10ml
價格¥ 1411
特異性
該抗體是針對DKK1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別DKK1。
用法
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時,沒有明顯的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
Nature Communications | Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953] |
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in … [Pubmed: 33065276] |
Oxid Med Cell Longev | Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats [34373766] |
注:產(chǎn)品僅用于科研