人銜接蛋白酶活化因子1(APAF1)ELISA 檢測(cè)試劑盒,人銜接蛋白酶活化因子1(APAF1)ELISA 檢測(cè)試劑盒
人銜接蛋白酶活化因子1(APAF1)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
人銜接蛋白酶活化因子1(APAF1)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12819B 小鼠單核細(xì)胞趨化蛋白2(MCP-2/CCL8)elisa試劑盒 Mouse monocyte chemotactic protein 2,MCP-2 Elisa試劑盒
RS12820B 小鼠單核細(xì)胞趨化蛋白3(MCP-3/CCL7)elisa試劑盒 Mouse monocyte chemotactic protein 3,MCP-3 Elisa試劑盒
RS12821B 小鼠基質(zhì)金屬蛋白酶1(MMP-1)elisa試劑盒 Mouse matrix metalloproteinase 1,MMP-1 Elisa試劑盒
RS12822B 小鼠基質(zhì)金屬蛋白酶10(MMP-10)elisa試劑盒 Mouse matrix metalloproteinase 10,MMP-10 Elisa試劑盒
RS12823B 小鼠基質(zhì)金屬蛋白酶13(MMP-13)elisa試劑盒 Mouse matrix metalloproteinase 13,MMP-13 Elisa試劑盒
RS12824B 小鼠基質(zhì)金屬蛋白酶7(MMP-7)elisa試劑盒 Mouse matrix metalloproteinase 7,MMP-7 Elisa試劑盒
RS12825B 小鼠基質(zhì)金屬蛋白酶8/中性粒細(xì)胞膠原酶(MMP-8)elisa試劑盒 Mouse matrix metalloproteinase 8/Neutrophil collagenase,MMP-8 Elisa試劑盒
RS12826B 小鼠胎盤生長(zhǎng)因子(PLGF)elisa試劑盒 Mouse placenta growth factor,PLGF Elisa試劑盒
RS12827B 小鼠干細(xì)胞因子受體(SCFR)elisa試劑盒 Mouse Stem Cell Factor Receptor,SCFR Elisa試劑盒
RS12828B 小鼠血管生成素受體Tie1(ANG-R-Tie1)elisa試劑盒 Mouse angiopoietin receptor Tie1,ANG-R-Tie1 Elisa試劑盒
RS12829B 小鼠血管生成素受體Tie2(ANG-R-Tie2)elisa試劑盒 Mouse angiopoietin receptor Tie2,ANG-R-Tie2 Elisa試劑盒
RS12830B 小鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)elisa試劑盒 Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 Elisa試劑盒
RS12831B 小鼠基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)elisa試劑盒 Mouse tissue inhibitors of metalloproteinase 3,TIMP-3 Elisa試劑盒
RS12832B 小鼠基質(zhì)金屬蛋白酶抑制因子4(TIMP-4)elisa試劑盒 Mouse tissue inhibitors of metalloproteinase 4,TIMP-4 Elisa試劑盒
RS12833B 小鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體1(TRAIL-R1)elisa試劑盒 Mouse tumor necrosis factor-related apoptosis-inducing ligand 1,TRAIL R1 Elisa試劑盒
RS12834B 小鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)elisa試劑盒 Mouse tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL R3 Elisa試劑盒
RS12835B 小鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體4(TRAIL-R4)elisa試劑盒 Mouse tumor necrosis factor-related apoptosis-inducing ligand 4,TRAIL R4 Elisa試劑盒
RS12836B 小鼠可溶性腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(sTRAIL)elisa試劑盒 Mouse soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL Elisa試劑盒
RS12837B 小鼠尿激酶型纖溶酶原激活物(uPA)elisa試劑盒 Mouse urokinase plasminogen activator,uPA Elisa試劑盒
RS12838B 小鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒 Mouse Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR Elisa試劑盒
RS12839B 小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子B(VEGF-B)elisa試劑盒 Mouse Vascular Endothelial cell Growth Factor B,VEGF-B Elisa試劑盒
RS12840B 小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子C(VEGF-C)elisa試劑盒 Mouse Vascular Endothelial cell Growth Factor C,VEGF-C Elisa試劑盒
RS12841B 小鼠白介素12(IL-12/P40)elisa試劑盒 Mouse interleukin 12,IL-12/P40 Elisa試劑盒
RS12842B 小鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子D(VEGF-D)elisa試劑盒 Mouse Vascular Endothelial cell Growth Factor D,VEGF-D Elisa試劑盒ab15845 Mouse monoclonal [CDFX] to Golgin 97 200ul 3450
ab15846 Mouse monoclonal to EEA1 - Early Endosome Marker 200ul 3738
ab15847 Mouse monoclonal [1AD9] to CKII alpha 250ul 3450
ab15848 Mouse monoclonal to Casein Kinase 2 beta 125ul 3450
ab15850 Goat polyclonal to RNF8 100ug 3450
ab15851 Chicken polyclonal to alpha 1b Adrenergic Receptor 50ug 3450
ab15852 Chicken polyclonal to Arrestin C 50ug 3450
ab15853 Chicken polyclonal to ATP6J 50ug 3450
ab15854 Chicken polyclonal to CLECSF6 50ug 3450
ab15855 Chicken polyclonal to CPLX1 50ug 3450
ab15856 Chicken polyclonal to DGCR6 50ug 3450
ab15857 Chicken polyclonal to FHL3 50ug 3450
ab15858 Chicken polyclonal to GABARAPL2 50ug 3450
ab15859 Chicken polyclonal to GAS41 50ug 3450
ab15860 Chicken polyclonal to Ghrelin 50ug 3450
ab15861 Chicken polyclonal to Ghrelin 50ug 3450
ab15862 Chicken polyclonal to TFIIF 50ug 3450
ab15863 Chicken polyclonal to HDL 100ug 3450
ab15864 Chicken polyclonal to Human IgA 50ug 3450
ab15865 Chicken polyclonal to IGFBP6 50ug 3450
ab15866 Chicken polyclonal to Human + Mouse + Rat IgG Fc fragment 100ug 3450
ab15868 Chicken polyclonal to IL21 50ug 3450
ab15869 Chicken polyclonal to KCNC2 50ug 3450
ab15870 Chicken polyclonal to LDL 100ug 3450
ab15871 Chicken polyclonal to MRPL28 50ug 3450
ab15872 Chicken polyclonal to MPHOSPH6 50ug 3450
ab15873 Chicken polyclonal to NHP2L1 50ug 3450
ab15874 Chicken polyclonal to NRP2 50ug 3450
ab15875 Chicken polyclonal to PAFAH1B2 50ug 3450
ab15876 Chicken polyclonal to PFDN1 50ug 3450
ab15877 Chicken polyclonal to PSMD8 50ug 3450
ab15879 Chicken polyclonal to Peptide YY 50ug 3450
ab15880 Chicken polyclonal to Rac1 50ug 3450
ab15881 Chicken polyclonal to RGS19 50ug 3450
ab15884 Chicken polyclonal to T54 50ug 3450
ab15893 Rabbit polyclonal to AMCR 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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