人輪狀病毒抗原(RV Ag)ELISA 檢測試劑盒,人輪狀病毒抗原(RV Ag)ELISA 檢測試劑盒
人輪狀病毒抗原(RV Ag)ELISA 檢測試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
人輪狀病毒抗原(RV Ag)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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RS11671B 大鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)elisa試劑盒 Rat Tumor necrosis factor soluble receptor Ⅰ,TNFsR-Ⅰ Elisa試劑盒
RS11672B 大鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)elisa試劑盒 Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ Elisa試劑盒
RS11673B 大鼠腫瘤壞死因子β(TNF-β)elisa試劑盒 Rat Tumor necrosis factor β,TNF-β Elisa試劑盒
RS11674B 大鼠血小板生成素(TPO)elisa試劑盒 Rat Thrombopoietin,TPO Elisa試劑盒
RS11675B 大鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體1(TRAIL-R1)elisa試劑盒 Rat tumor necrosis factor-related apoptosis-inducing ligand 1,TRAIL R1 Elisa試劑盒
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ab1224 Rabbit polyclonal to Cyclin D1 100ul 3450
ab12241 Rabbit polyclonal to Secretogranin II 100ul 3450
ab12242 Rabbit polyclonal to Chromogranin B 100ul 3450
ab12243 Chicken polyclonal to Raspberry Bushy Dwarf Virus Coat Protein 125ug 3450
ab12244 Chicken polyclonal to Tomato Spotted Wilt Virus Core Protein 125ug 3450
ab12246 Mouse monoclonal to Staphylococcus Enterotoxin A 100ug 3450
ab12248 Mouse monoclonal to Lipoteichoic Acid 500ug 3450
ab12249 Rabbit polyclonal to TRPC6 50ul 3450
ab1225 Mouse monoclonal [BB12 + CB19] to CD3 + CD19 (Dual color: FITC + PE) 500ul 3450
ab12251 Mouse monoclonal to Beta Lactamase 500ug 3450
ab12252 Mouse monoclonal to Beta Lactamase 500ug 3450
ab12253 Rabbit polyclonal to Respiratory Syncytial Virus 50ug 3450
ab12254 Mouse monoclonal [AS61] to EDG3 25ug 3450
ab12258 Rabbit polyclonal to cGKI 50ul 3450
ab1226 Mouse monoclonal [BB12 + LN3] to CD3 + HLA-DR (Dual color: FITC + PE) 500ul 3450
ab12261 Rabbit polyclonal to Nurr1 100ug 3450
ab12265 Rabbit polyclonal to beta Amyloid 1-40 100ul 3450
ab12266 Mouse monoclonal [DE2B4] to Amyloid Precursor Protein 1ml 3450
ab12267 Rabbit polyclonal to beta Amyloid 1-42 100ul 3450
ab12268 Rabbit polyclonal to Amyloid beta precursor protein 100ul 3450
ab12269 Rabbit polyclonal to Amyloid beta precursor protein 100ul 3450
ab1227 Rabbit polyclonal to Biotin 100ug 3450
ab12270 Rabbit polyclonal to Amyloid beta precursor protein 100ul 3450
ab12271 Rabbit polyclonal to Amyloid beta precursor protein 100ul 3450
ab12272 Rabbit polyclonal to Presenilin 1 100ul 3450
ab12273 Rabbit polyclonal to Presenilin 1 100ul 3450
ab12274 Rabbit polyclonal to GAP43 - Neuronal Marker 100ul 3450
ab12275 Rabbit polyclonal to Prohormone Convertase 2 100ul 3450
ab12277 Rabbit polyclonal to Mannosidase II - Golgi Marker 100ul 3450
ab1228 Sheep polyclonal to Biotin 100ug 3450
ab12281 Rabbit polyclonal to Cdc27 (phospho T244) 100ug 3450
ab12282 Rabbit polyclonal to Apc6 (phospho T580) 100ug 3450
ab12286 Rabbit polyclonal to Drosha 50ul 1380
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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