大鼠磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶(pERK)ELISA 檢測(cè)試劑盒,大鼠磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶(pERK) ELISA 檢測(cè)試劑盒
大鼠磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶(pERK) ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶(pERK)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12650B 雞堿性成纖維細(xì)胞生長因子(bFGF)elisa試劑盒 Chicken basic fibroblast growth factor,bFGF Elisa試劑盒
RS12651B 雞可溶性細(xì)胞間粘附分子1(sICAM-1)elisa試劑盒 Chicken Soluble InteFAellular Adhesion Molecule-1,sICAM-1 Elisa試劑盒
RS12652B 雞可溶性E選擇素(sE-selectin)elisa試劑盒 Chicken soluble E-selectin,sE-selectin Elisa試劑盒
RS12653B 雞表皮生長因子(EGF)elisa試劑盒 Chicken Epidermal growth factor,EGF Elisa試劑盒
RS12654B 雞胰島素樣生長因子1(IGF-1)elisa試劑盒 Chicken Insulin-like growth factors 1,IGF-1 Elisa試劑盒
RS12655B 雞生長因子(GH)elisa試劑盒 Chicken growth hormone,GH Elisa試劑盒
RS12656B 雞乙酰乙酸檢測(cè)(ACAC)elisa試劑盒 Chicken Acetoacetic acid,ACAC Elisa試劑盒
RS12657B 雞傳染性鼻炎抗體(IC)elisa試劑盒 Chicken infectious coryza,ic Elisa試劑盒
RS12658B 雞透明質(zhì)酸(HA)elisa試劑盒 Chicken Hyaluronic acid,HA Elisa試劑盒
RS12659B 雞硫酸類肝素(HS)elisa試劑盒 Chicken heparan sulfate,HS Elisa試劑盒
RS12660B 雞催乳素(PRL)elisa試劑盒 Chicken prolactin,PRL Elisa試劑盒
RS12661B 雞白血病病毒抗原(ALV-AG)elisa試劑盒 Chicken Avian Leukosis Virus AG,ALV-AG Elisa試劑盒
RS12662B 雞促卵泡素(FSH)elisa試劑盒 Chicken follicle-stimulating hormone,FSH Elisa試劑盒
RS12663B 雞促黃體激素(LH)elisa試劑盒 Chicken Luteinizing Hormone,LH Elisa試劑盒
RS12664B 雞γ干擾素(IFN-γ)elisa試劑盒 Chicken Interferon γ ,IFN-γ Elisa試劑盒
RS12665B 雞白介素6(IL-6)elisa試劑盒 Chicken Interleukin 6,IL-6 Elisa試劑盒
RS12666B 雞碳酸酐酶(CA)elisa試劑盒 Chicken carbonic anhydrase,CA Elisa試劑盒
RS12667B 雞抗凝血酶Ⅲ抗體(AT-Ⅲ)elisa試劑盒 Chicken Anti-Thrombin Ⅲ,AT-Ⅲ Elisa試劑盒
RS12668B 雞熱休克蛋白40(HSP-40)elisa試劑盒 Chicken Heat Shock Protein 40,HSP-40 Elisa試劑盒
RS12669B 雞熱休克蛋白20(HSP-20)elisa試劑盒 Chicken Heat Shock Protein 20,HSP-20 Elisa試劑盒
RS12670B 雞熱休克蛋白60(Hsp-60)elisa試劑盒 Chicken Heat Shock Protein 60,HSP-60 Elisa試劑盒 ab28569 Rabbit polyclonal to Kallikrein 13 - Kallikrein loop 100ug 3450
ab2857 Mouse polyclonal to XRCC4 50ul 3450
ab28570 Rabbit polyclonal to Kallikrein 15 - Kallikrein loop 100ug 3450
ab28571 Rabbit polyclonal to Proprotein Convertase 2 - Aminoterminal end 100ug 3450
ab28572 Rabbit polyclonal to Proprotein Convertase 2 - Homo-B domain 100ug 3450
ab28573 Rabbit polyclonal to Proprotein convertase PC4 - Aminoterminal end 100ug 3450
ab28575 Rabbit polyclonal to HOXB13 50ul 3450
ab28577 Rabbit polyclonal to Rad51 100ul 3450
ab28578 Rabbit polyclonal to Somatostatin Receptor 4 100ul 3450
ab28579 Rabbit polyclonal to HSV1 + HSV2 (FITC) 100ul 3450
ab28580 Rabbit polyclonal to Vaccinia Virus 100ul 3450
ab28584 Goat polyclonal to Parainfluenza Virus type 3 100ul 3450
ab28585 Rabbit polyclonal to PKR 100ug 3450
ab2859 Rabbit polyclonal to SRC1 100ug 3450
ab28596 ADAMTS7 antibody 50ug 3450
ab286 Mouse monoclonal [6A12] to Estrogen Receptor beta 100ug 3450
ab2860 Mouse monoclonal [2D1] to Calmodulin 200ul 3450
ab2861 Mouse monoclonal [2A7-A1] to SERCA2 ATPase 100ul 3450
ab28618 Rabbit polyclonal to Somatostatin Receptor 5 100ul 3450
ab2862 Mouse monoclonal [1A] to DHPR alpha 1 400ul 3450
ab28624 Rabbit polyclonal to VPAC2 100ul 3450
ab28627 Rabbit polyclonal to CCKAR 100ul 3450
ab28628 Rabbit polyclonal to C21orf18 50ug 3450
ab28629 Rabbit polyclonal to RING2 / RING1B / RNF2 100ug 3450
ab28630 Rabbit polyclonal to SHOX2 200ug 3450
ab28631 Rabbit polyclonal to KCNN3 50ug 3450
ab28632 Rabbit polyclonal to TBC1D10B 100ug 3450
ab28633 Rabbit polyclonal to TBP like protein TLP 100ug 3450
ab28634 Rabbit polyclonal to Tbx4 50ug 3450
ab28636 Rabbit polyclonal to RNF135 50ug 3450
ab28637 Rabbit polyclonal to LASS3 50ug 3450
ab28638 Rabbit polyclonal to PRDM14 100ug 3450
ab28639 Rabbit polyclonal to RAB14 50ug 3450
ab2864 Mouse monoclonal [20A] to Calcium channel L type DHPR alpha 2 subunit 100ul 3450
ab28641 Rabbit polyclonal to KCNIP4 50ug 3450
ab28642 Rabbit polyclonal to PHKG2 50ug 3450
ab28643 Rabbit polyclonal to MSY2/YBOX2 100ug 3450
ab28647 Rabbit polyclonal to Chp1 100ug 3450
ab2865 Mouse monoclonal [2D12] to Phospholamban 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
關(guān)于大鼠磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶(pERK) ELISA試劑盒說明書、等相關(guān)資料可向我們銷售人員咨詢索要。