大鼠Ⅱ型膠原(Col Ⅱ)ELISA 檢測試劑盒,大鼠Ⅱ型膠原(Col Ⅱ)ELISA 檢測試劑盒
大鼠Ⅱ型膠原(Col Ⅱ)ELISA 檢測試劑盒*試驗原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠Ⅱ型膠原(Col Ⅱ) ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12497B 豚鼠單核細(xì)胞趨化蛋白4(MCP-4/CCL13)elisa試劑盒 Guinea Pig monocyte chemotactic protein 4,MCP-4 Elisa試劑盒
RS12498B 豚鼠卵清蛋白特異性IgG(OVA sIgG)elisa試劑盒 Guinea pig ovalbumin specific IgG,OVA sIgG Elisa試劑盒
RS12499B 豚鼠鉤端螺旋體IgG(Lebtospira)elisa試劑盒 Guinea pig Lebtospira IgG Elisa試劑盒
RS12500B 豚鼠白三烯D4(LTD4)elisa試劑盒 Guinea pig leukotriene D4,LT-D4 Elisa試劑盒
RS12501B 豚鼠主要組織相容性復(fù)合體(MHC/GPLA)elisa試劑盒 Guinea pig major histocompatibility complex,MHC/GPLA Elisa試劑盒
RS12502B 豚鼠卵清蛋白特異性IgE(OVA sIgE)elisa試劑盒 Guinea pig ovalbumin specific IgE,OVA sIgE Elisa試劑盒
RS12503B 豚鼠白介素12(IL-12/P70)elisa試劑盒 Guinea pig Interleukin-12,IL-12/P70 Elisa試劑盒
RS12504B 豚鼠血清一氧化氮(NO)elisa試劑盒 guinea pig nitric oxide,NO Elisa試劑盒
RS12505B 豚鼠內(nèi)皮型一氧化氮合成酶(eNOS)elisa試劑盒 Guinea Pig Endothelial nitric oxide synthase,eNOS Elisa試劑盒
RS12506B 豚鼠降鈣素基因相關(guān)肽(CGRP)elisa試劑盒 Guinea Pig calcitonin gene related peptide,CGRP Elisa試劑盒
RS12507B 豚鼠纖溶酶抗纖溶酶復(fù)合物(PAP)elisa試劑盒 Guinea Pig plasmin-antiplasmin complex,PAP Elisa試劑盒
RS12508B 豚鼠超氧化物歧化酶(SOD)elisa試劑盒 Guinea Pig Super Oxidase Dimutase,SOD Elisa試劑盒
RS12509B 豚鼠過氧化脂質(zhì)/乳過氧化物酶(LPO)elisa試劑盒 Guinea Pig lipid peroxlde,LPO Elisa試劑盒
RS12510B 豚鼠晚期糖基化終末產(chǎn)物(AGEs)elisa試劑盒 Guinea Pig advanced glycation end products,AGEs Elisa試劑盒
RS12511B 豬腸三葉因子(ITF)elisa試劑盒 PoFAine Intestinal trefoil factor,ITF Elisa試劑盒
RS12512B 豬β干擾素(IFN-β/IFNB)elisa試劑盒 PoFAine Interferon β,IFN-β/IFNB Elisa試劑盒
RS12513B 豬白介素1α(IL-1α)elisa試劑盒 PoFAine Interleukin 1α ,IL-1α Elisa試劑盒
RS12514B 豬血管內(nèi)皮鈣粘著蛋白復(fù)合體(VE-cad)elisa試劑盒 PoFAine Vascular Endothelial-Cadherin Complex,VE-cad Elisa試劑盒
RS12515B 豬凋亡相關(guān)因子(FAS/CD95)elisa試劑盒 PoFAine Factor-related Apoptosis,FAS Elisa試劑盒
RS12516B 豬血管生成素2(ANG-2)elisa試劑盒 PoFAine Angiopoietin 2,ANG-2 Elisa試劑盒
RS12517B 豬血管生成素受體Tie2(ANG-R-Tie2)elisa試劑盒 PoFAine angiopoietin receptor Tie2,ANG-R-Tie2 Elisa試劑盒
RS12518B 豬血管生成素1(ANG-1)elisa試劑盒 PoFAine Angiopoietin 1,ANG-1 Elisa試劑盒
RS12519B 豬可溶性細(xì)胞間粘附分子1(sICAM-1)elisa試劑盒 PoFAine Soluble InteFAellular Adhesion Molecule-1,sICAM-1 Elisa試劑盒
RS12520B 豬主要組織相容性復(fù)合體Ⅱ類(MHCⅡ/SLAⅡ)elisa試劑盒 swine major histocompatibility complexⅡ,MHCⅡ/SLAⅡ Elisa試劑盒 ab28304 Rabbit polyclonal to ADAM23 - Propeptide domain 100ug 3450
ab28305 Mouse monoclonal [M73] to Adenovirus E1A 100ug 3450
ab28307 Rabbit polyclonal to Kallikrein 11 - Kallikrein loop 100ug 3450
ab28309 Rabbit polyclonal to Kallikrein 7 - Kallikrein loop 100ug 3450
ab2831 Rabbit polyclonal to AIB1 - ChIP Grade 100ug 3450
ab28310 Rabbit polyclonal to Kallikrein 8 - Kallikrein loop 100ug 3450
ab28311 Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Aminoterminal end catalytic domain 100ug 3450
ab28312 Sheep polyclonal to Eph receptor B1 250ug 3450
ab28313 Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Carboxyterminal end catalytic domain 100ug 3450
ab28314 Sheep polyclonal to Eph receptor B1 250ug 3450
ab28315 Mouse monoclonal [AS17] to IL1RA, prediluted (FITC) 100tests 3450
ab28316 Sheep polyclonal to LEF1 100ug 3450
ab28318 Rabbit polyclonal to Plasminogen 100ul 3450
ab28319 Rabbit polyclonal to Plasminogen 500ug 3450
ab2832 Rabbit polyclonal to CBP - ChIP Grade 100ug 3450
ab28320 Mouse monoclonal [5A6] to Low Density LRP 200ug 3450
ab28322 Mouse monoclonal [MEM-12] to HLA DR 100ug 3450
ab28323 Mouse monoclonal [MEM-12] to HLA DR (FITC) 100tests 3450
ab28324 Sheep polyclonal to ErbB 2 250ug 3450
ab28325 Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Cytoplasmic domain 100ug 3450
ab28326 Rabbit polyclonal to Antiplasmin 100ug 3450
ab28327 Mouse monoclonal [MEM-12] to HLA DR (Phycoerythrin) 100tests 3450
ab28328 Mouse monoclonal [MEM-78] to CD10 (Phycoerythrin) 100tests 3450
ab28329 Mouse monoclonal [MEM-55] to CD45RB (Phycoerythrin) 100tests 3450
ab2833 Rabbit polyclonal to mTOR 50ug 3450
ab28330 Rabbit polyclonal to HGFA Inhibitor 1 - Aminoterminal end 100ug 3450
ab28331 Mouse monoclonal [MEM-55] to CD45RB (Allophycocyanin) 100tests 3450
ab28332 Rabbit polyclonal to HGFA Inhibitor 1 - KDI insert 100ug 3450
ab28333 Rabbit polyclonal to HGFA Inhibitor 1 - Cytoplasmic domain 100ug 3450
ab28334 Rabbit polyclonal to HGFA Inhibitor 2 - Between KD1 and KD2 100ug 3450
ab28335 Mouse monoclonal [MEM-188] to NCAM (Allophycocyanin) 100tests 3450
ab28336 Rabbit polyclonal to HGFA Inhibitor 2 - Cytoplasmic domain 100ug 3450
ab28337 Mouse monoclonal [EDU-2] to CD4 (Allophycocyanin) 100tests 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實驗中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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