大鼠鈣調(diào)素(CAM)ELISA 檢測試劑盒,大鼠鈣調(diào)素(CAM) ELISA 檢測試劑盒
大鼠鈣調(diào)素(CAM)ELISA 檢測試劑盒*試驗原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠鈣調(diào)素(CAM)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12538B 豬透明質(zhì)酸(HA)elisa試劑盒 PoFAine Hyaluronic acid,HA Elisa試劑盒
RS12539B 豬瘦素(LEP)elisa試劑盒 PoFAine Leptin,LEP Elisa試劑盒
RS12540B 豬生長抑素(SS)elisa試劑盒 PoFAine Somatostatin,SS Elisa試劑盒
RS12541B 豬生長激素(GH)elisa試劑盒 PoFAine growth hormone,GH Elisa試劑盒
RS12542B 豬皮質(zhì)酮/腎上腺酮(CORT)elisa試劑盒 PoFAine Corticosterone,CORT Elisa試劑盒
RS12543B 豬皮質(zhì)醇(Cortisol)elisa試劑盒 PoFAine Cortisol Elisa試劑盒
RS12544B 豬腦鈉素/腦鈉尿肽(BNP)elisa試劑盒 PoFAine brain natriuretic peptide,BNP Elisa試劑盒
RS12545B 豬內(nèi)皮素1(ET-1)elisa試劑盒 PoFAine endothelin 1,ET-1 Elisa試劑盒
RS12546B 豬免疫球蛋白M(IgM)elisa試劑盒 PoFAine Immunoglobulin M,IgM Elisa試劑盒
RS12547B 豬免疫球蛋白G(IgG)elisa試劑盒 PoFAine Immunoglobulin G,IgG Elisa試劑盒
RS12548B 豬免疫球蛋白E(IgE)elisa試劑盒 PoFAine Immunoglobulin E,IgE Elisa試劑盒
RS12549B 豬可溶性血管細(xì)胞粘附分子1(sVCAM-1)elisa試劑盒 PoFAine soluble vascuolar cell adhesion molecules 1,sVCAM-1 Elisa試劑盒
RS12550B 豬巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)elisa試劑盒 PoFAine macrophage inflammatory protein 1α,MIP-1α Elisa試劑盒
RS12551B 豬角化細(xì)胞生長因子(KGF)elisa試劑盒 PoFAine Keratinocyte Growth Factor,KGF Elisa試劑盒
RS12552B 豬基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)elisa試劑盒 PoFAine tissue inhibitors of metalloproteinase 1,TIMP-1 Elisa試劑盒
RS12553B 豬基質(zhì)金屬蛋白酶9(MMP-9)elisa試劑盒 PoFAine matrix metalloproteinase 9,MMP-9 Elisa試劑盒
RS12554B 豬睪酮(T)elisa試劑盒 PoFAine Testoterone,T Elisa試劑盒
RS12555B 豬肝細(xì)胞生長因子(HGF)elisa試劑盒 PoFAine hepatocyte growth factor,HGF Elisa試劑盒
RS12556B 豬γ干擾素(IFN-γ)elisa試劑盒 PoFAine Interferon γ ,IFN-γ Elisa試劑盒
RS12557B 豬端粒酶(TE)elisa試劑盒 PoFAine omerase,TE Elisa試劑盒
RS12558B 豬促生長激素釋放激素(GHRH)elisa試劑盒 PoFAine growth hormone releasing hormone,GHRH Elisa試劑盒
RS12559B 豬促卵泡素(FSH)elisa試劑盒 PoFAine Follicle-stimulating hormone,FSH Elisa試劑盒ab13809 Rabbit polyclonal to Bacillus anthracis protective antigen 200ul 3450
ab13810 Rabbit polyclonal to TRIF 100ul 3450
ab13811 Rabbit polyclonal to MARCKS (phospho S152 + S153) 50ul 3450
ab13812 Rabbit polyclonal to Pelli 200ul 3450
ab13813 Mouse monoclonal [9E1237] to BEX2 100ug 3450
ab13814 Mouse monoclonal [5F502.2] to Bacillus anthracis lethal factor 100ug 3450
ab13817 Rabbit polyclonal to Smac / Diablo 100ug 3450
ab13818 Mouse monoclonal [5B789] to p73 200ul 3450
ab13819 Goat polyclonal to MSH5 100ug 2826
ab1382 Goat polyclonal to DAP Kinase 2 100ug 3450
ab13820 Goat polyclonal to TXNDC5 100ug 3450
ab13821 Goat polyclonal to NEDL2 100ug 2826
ab13822 Chicken polyclonal to beta Actin - Loading Control 100ug 3450
ab13824 Mouse monoclonal [35C1] to Aurora A 100ug 3795
ab13825 Mouse monoclonal [2E9] to RNase L 100ug 3450
ab13826 Mouse monoclonal to RPE65 100ul 3450
ab13828 Rabbit polyclonal to SLC31A1 100ul 3450
ab13829 Mouse monoclonal [H21] to Hepatitis C Virus antigen 100ul 3450
ab1383 Goat polyclonal to DDAH2 100ug 3450
ab13830 Mouse monoclonal [H23] to Hepatitis C Virus antigen 100ul 3450
ab13831 Mouse monoclonal [H24] to Hepatitis C Virus antigen 100ul 3450
ab13832 Mouse monoclonal [H25] to Hepatitis C Virus antigen 100ul 3450
ab13833 Mouse monoclonal [H26] to Hepatitis C Virus antigen 100ul 3450
ab13834 Rabbit polyclonal to HA tag 100ug 3450
ab13836 Rabbit polyclonal to Myc tag 100ug 3450
ab13840 Rabbit polyclonal to DDX4 / MVH - Primordial Germ Cell Marker 100ug 3795
ab13840 Rabbit polyclonal to DDX4 / MVH - Primordial Germ Cell Marker 25ug 1380
ab13847 Rabbit polyclonal to active Caspase 3 100ug 3737
ab1385 Mouse monoclonal [LKH15] to Cytokeratin 15 500ul 3450
ab13851 Rabbit polyclonal to NUR77 200ul 3450
ab13852 Rabbit polyclonal to DNA PKcs 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實驗中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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