大鼠肌球蛋白(Myosin)ELISA 檢測(cè)試劑盒,大鼠肌球蛋白(Myosin)ELISA 檢測(cè)試劑盒
大鼠肌球蛋白(Myosin)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
大鼠肌球蛋白(Myosin)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12457B 兔子可溶性P選擇素(sP-selectin)elisa試劑盒 rabbit soluble P-selectin,sP-selectin Elisa試劑盒
RS12458B 兔子Ⅰ型前膠原羧基端肽(PⅠCP)elisa試劑盒 rabbit Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP Elisa試劑盒
RS12459B 兔子Ⅰ型膠原(Col Ⅰ)elisa試劑盒 rabbit Collagen Type Ⅰ,Col Ⅰ Elisa試劑盒
RS12460B 兔子Ⅱ型膠原(Col Ⅱ)elisa試劑盒 rabbit Collagen Type Ⅱ,Col Ⅱ Elisa試劑盒
RS12461B 兔子Ⅲ型前膠原肽(PⅢNP)elisa試劑盒 rabbit N-terminal procollagen Ⅲ propeptide,PⅢNP Elisa試劑盒
RS12462B 兔子Ⅲ型前膠原氨基端肽(PⅢNT)elisa試劑盒 rabbit procollagen Ⅲ N-terminal peptide,PⅢNT Elisa試劑盒
RS12463B 兔子凝血因子Ⅱ(FⅡ)elisa試劑盒 Rabbit coagulation factor Ⅱ,FⅡ Elisa試劑盒
RS12464B 兔子Ⅲ型膠原(Col Ⅲ)elisa試劑盒 rabbit Collagen Type Ⅲ,Col Ⅲ Elisa試劑盒
RS12465B 兔子C反應(yīng)蛋白(CRP)elisa試劑盒 rabbit C-Reactive Protein,CRP Elisa試劑盒
RS12466B 兔子內(nèi)皮型一氧化氮合成酶3(eNOS-3)elisa試劑盒 Rabbit Endothelial nitric oxide synthase 3,eNOS-3 Elisa試劑盒
RS12467B 兔子膽固醇酯轉(zhuǎn)移蛋白(CETP)elisa試劑盒 Rabbit cholest erolester transfer protein,CETP Elisa試劑盒
RS12468B 兔子可溶性粘附分子(Sam)elisa試劑盒 Rabbit soluble adhesion molecules,Sam Elisa試劑盒
RS12469B 兔子抗中性粒細(xì)胞顆??贵w(ANGA)elisa試劑盒 Rabbit anti-neutrophil granules antibody,ANGA Elisa試劑盒
RS12470B 兔子孕激素/孕酮(PROG)elisa試劑盒 Rabbit Progesterone,PROG Elisa試劑盒
RS12471B 兔子膠原酶II(Collagenase II)elisa試劑盒 Rabbit Collagenase Type II Elisa試劑盒
RS12472B 兔子膠原酶I(Collagenase I)elisa試劑盒 Rabbit Collagenase I Elisa試劑盒
RS12473B 兔子腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)elisa試劑盒 Rabbit tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 Elisa試劑盒
RS12474B 兔子巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)elisa試劑盒 Rabbit Macrophage Inflammatory Protein-1α,MIP-1α Elisa試劑盒
RS12475B 兔子神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)elisa試劑盒 Rabbit glial fibrillary acidic protein,GFAP Elisa試劑盒
RS12476B 豚鼠白介素4(IL-4)elisa試劑盒 Guinea pig interleukin 4,IL-4 Elisa試劑盒
RS12477B 豚鼠免疫球蛋白G(IgG)elisa試劑盒 Guinea pig Immunoglobulin G,IgG Elisa試劑盒
RS12478B 豚鼠血清總補(bǔ)體(CH50)elisa試劑盒 Guinea Pig 50% complement hemolysis,CH50 Elisa試劑盒
RS12479B 豚鼠組胺(HIS)elisa試劑盒 guinea pig Histamine,HIS Elisa試劑盒 ab25516 Rat monoclonal [5H4] to IL2 Receptor beta (Allophycocyanin) 100ug 3450
ab25517 Armenian Hamster monoclonal [2E7] to Integrin alpha E (Phycoerythrin) 100ug 3450
ab25519 Rat monoclonal [I3/2.3] to CD45 (Allophycocyanin) 100ug 3450
ab2552 Rabbit polyclonal to Caspase 6 50ug 3450
ab25520 Rat monoclonal [I3/2.3] to CD45 (PE/Cy7) 100ug 3450
ab25521 Rat monoclonal [C455.1F] to CD45RC (Phycoerythrin) 100ug 3450
ab25522 Armenian Hamster monoclonal [HM79-12] to CD79b (Phycoerythrin) 100ug 3450
ab25523 Rat monoclonal [RA3-6B2] to CD45R (Allophycocyanin) 100ug 3450
ab25524 Rat monoclonal [3C4] to ICAM2 (Phycoerythrin) 100ug 3450
ab25525 Rat monoclonal [RA3-6B2] to CD45R (PE/Cy5) 100ug 3450
ab25526 Rat monoclonal [RA3-6B2] to CD45R (Cy5) 100ug 3450
ab25527 Mouse monoclonal [RDR34] to RLA DR (Phycoerythrin) 100ug 3450
ab25528 Rat monoclonal [RA3-6B2] to CD45R (PE/Cy7) 100ug 3450
ab25529 Armenian Hamster monoclonal [H1.2F3] to CD69 (PE/Cy7) 100ug 3450
ab2553 Mouse monoclonal [C502S] to Caspase 8 50ug 3450
ab25530 Syrian Hamster monoclonal [500-A2] to CD3 epsilon (PE/Cy5) 100ug 3450
ab25531 Rat monoclonal [C363.29B] to CD3 epsilon (PE/Cy5) 100ug 3450
ab25532 Rat monoclonal [GK1.5] to CD4 (Cy5) 100ug 3450
ab25533 Rat monoclonal [M1/70] to CD11b (PE/Cy5) 100ug 3450
ab25534 Rat monoclonal [7D4] to IL2 Receptor alpha (PE/Cy5) 100ug 3450
ab25535 Mouse monoclonal [PPT3] to CD3 epsilon (PE/Cy5) 100ug 3450
ab25536 Mouse monoclonal [76-2-11] to CD8 alpha (PE/Cy5) 100ug 3450
ab25537 Mouse monoclonal [CT-3] to CD3 (PE/Cy5) 100ug 3450
ab25538 Mouse monoclonal [2C4] to RLA DQ (Phycoerythrin) 100ug 3450
ab25539 Sheep polyclonal to FITC (Phycoerythrin) 100ug 3450
ab2554 Rabbit polyclonal to DAP1 50ug 3450
ab25540 Mouse monoclonal [143-30] to CD55 (Phycoerythrin) 1ml 3450
ab25541 Mouse monoclonal [SBZAP] to ZAP70 100ug 3450
ab25542 Mouse monoclonal [B-Ly1] to CD20 (Allophycocyanin) 100tests 3450
ab25543 Mouse monoclonal [RVS10] to Transferrin Receptor (Phycoerythrin) 1ml 3450
ab25544 Rat monoclonal [30-H12] to Thy1.2 (PE/Cy5) 100ug 3450
ab25545 Mouse monoclonal [SB70] to ZAP70 (Biotin) 100ug 3450
ab25546 Mouse monoclonal [SBZAP] to ZAP70 (FITC) 100ug 3450
ab25547 Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db (Phycoerythrin) 100ug 3450
ab25548 Rat monoclonal [6D5] to CD19 (PE/Cy5.5) 100ug 3450
ab25549 Mouse monoclonal [SJ25-C1] to CD19 (Phycoerythrin) 500ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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