大鼠維生素C(VC)ELISA 檢測試劑盒,大鼠維生素C(VC)ELISA 檢測試劑盒
大鼠維生素C(VC)ELISA 檢測試劑盒*試驗原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進行檢測。先將ZR和生物素標(biāo)記的抗體同時溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠維生素C(VC)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12865B 小鼠血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)elisa試劑盒 Mouse Vascuolar cell adhesion molecule 1,VCAM-1 Elisa試劑盒
RS12866B 小鼠血小板生成素(TPO)elisa試劑盒 Mouse Thrombopoietin,TPO Elisa試劑盒
RS12867B 小鼠腫瘤壞死因子β(TNF-β)elisa試劑盒 Mouse Tumor necrosis factor β,TNF-β Elisa試劑盒
RS12868B 小鼠腫瘤壞死因子α(TNF-α)elisa試劑盒 Mouse Tumor necrosis factor α,TNF-α Elisa試劑盒
RS12869B 小鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)elisa試劑盒 Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ Elisa試劑盒
RS12870B 小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)elisa試劑盒 Mouse Tumor necrosis factor soluble receptor Ⅰ,TNFsR-Ⅰ Elisa試劑盒
RS12871B 小鼠轉(zhuǎn)化生長因子β1(TGF-β1)elisa試劑盒 Mouse Transforming Growth factor β1,TGF-β1 Elisa試劑盒
RS12872B 小鼠基質(zhì)細(xì)胞衍生因子1β(SDF-1β/CXCL12)elisa試劑盒 Mouse Stromal cell derived factor 1β,SDF-1β Elisa試劑盒
RS12873B 小鼠干細(xì)胞因子/肥大細(xì)胞生長因子(SCF/MGF)elisa試劑盒 Mouse Stem cell factor/mast cell growth factor,SCF/MGF Elisa試劑盒
RS12874B 小鼠可溶性白細(xì)胞分化抗原40配體(sCD40L)elisa試劑盒 Mouse Soluble Cluster of differentiation 40 ligand,sCD40L Elisa試劑盒
RS12875B 小鼠P選擇素(P-Selectin/CD62P)elisa試劑盒 Mouse P-Selectin Elisa試劑盒
RS12876B 小鼠血小板衍生生長因子AB(PDGF-AB)elisa試劑盒 Mouse Plaet-Derived Growth Factor AB,PDGF-AB Elisa試劑盒
RS12877B 小鼠血小板衍生生長因子可溶性受體α(PDGFsR-α)elisa試劑盒 Mouse Plaet-Derived Growth Factor Soluble Receptor α,PDGFsR-α Elisa試劑盒
RS12878B 小鼠抑瘤素M(OSM)elisa試劑盒 Mouse Oncostatin-M,OSM Elisa試劑盒
RS12879B 小鼠B因子(BF)elisa試劑盒 Mouse factor B,BF Elisa試劑盒
RS12880B 小鼠骨保護素(OPG)elisa試劑盒 Mouse Osteoprotegerin,OPG Elisa試劑盒
RS12881B 小鼠神經(jīng)營養(yǎng)因子4(NT-4)elisa試劑盒 Mouse Neurotrophin 4,NT-4 Elisa試劑盒
RS12882B 小鼠神經(jīng)營養(yǎng)因子3(NT-3)elisa試劑盒 Mouse Neurotrophin 3,NT-3 Elisa試劑盒
RS12883B 小鼠神經(jīng)生長因子(NGF)elisa試劑盒 Mouse Nerve growth factor,NGF Elisa試劑盒
RS12884B 小鼠基質(zhì)金屬蛋白酶3(MMP-3)elisa試劑盒 Mouse matrix metalloproteinase 3,MMP-3 Elisa試劑盒
RS12885B 小鼠基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)elisa試劑盒 Mouse matrix metalloproteinase 2/Gelatinase A,MMP-2 Elisa試劑盒
RS12886B 小鼠巨噬細(xì)胞炎性蛋白3β(MIP-3β/ELC/CCL19)elisa試劑盒 Mouse macrophage inflammatory protein 3β,MIP-3β Elisa試劑盒
RS12887B 小鼠巨噬細(xì)胞炎性蛋白3α(MIP-3α/CCL20)elisa試劑盒 Mouse Macrophage Inflammatory Protein 3α,MIP-3α Elisa試劑盒
RS12888B 小鼠巨噬細(xì)胞炎性蛋白2(MIP-2)elisa試劑盒 Mouse Macrophage Inflammatory Protein 2,MIP-2 Elisa試劑盒
RS12889B 小鼠巨噬細(xì)胞炎性蛋白1β(MIP-1β/CCL4)elisa試劑盒 Mouse Macrophage Inflammatory Protein 1β,MIP-1β Elisa試劑盒
RS12890B 小鼠巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)elisa試劑盒 Mouse Macrophage Inflammatory Protein 1α,MIP-1α Elisa試劑盒
RS12891B 小鼠巨噬細(xì)胞來源的趨化因子(MDC/CCL22)elisa試劑盒 Mouse Macrophage-Derived Chemokine,MDC Elisa試劑盒 ab26178 Chicken polyclonal to Integrin alpha 9 50ug 3450
ab2618 Mouse monoclonal [5C2] to ATM 100ug 3450
ab26182 Chicken polyclonal to TIAF1 50ug 3450
ab26183 Chicken polyclonal to DUSP15 50ug 3450
ab26184 Chicken polyclonal to Stanniocalcin 2 50ug 3450
ab26185 Chicken polyclonal to RPC62 50ug 3450
ab26187 Chicken polyclonal to TNFAIP1 50ug 3450
ab26188 Chicken polyclonal to POLDIP2 50ug 3450
ab26189 Chicken polyclonal to SCAND1 50ug 3450
ab2619 Rabbit polyclonal to FEN1 100ul 3450
ab26190 Rabbit polyclonal to Neurogenin 2 100ug 3450
ab26191 Chicken polyclonal to COMMD10 50ug 3450
ab26192 Chicken polyclonal to Liprin alpha 1 50ug 3450
ab26193 Chicken polyclonal to PTPN13 50ug 3450
ab26194 Chicken polyclonal to Cdc14B 50ug 3450
ab26195 Chicken polyclonal to TPTE 50ug 3450
ab26196 Chicken polyclonal to NOLA1 50ug 3450
ab26197 Rabbit polyclonal to eIF2 alpha 100ug 3450
ab26198 Chicken polyclonal to LOC51596 50ug 3450
ab26199 Chicken polyclonal to TGF beta induced factor 2 50ug 3450
ab2620 Rabbit polyclonal to Ku70 100ul 3450
ab26200 Chicken polyclonal to Myozenin 2 50ug 3450
ab26201 Chicken polyclonal to SNX2 50ug 3450
ab26202 Chicken polyclonal to Grancalcin 50ug 3450
ab26204 Chicken polyclonal to DAP13 50ug 3450
ab26205 Chicken polyclonal to SSBP1 - Mitochondrial Marker 50ug 3450
ab26206 Chicken polyclonal to N acetyl transferase 5 50ug 3450
ab26208 Rat monoclonal [RMP1-30] to PDCD1 100ug 3450
ab26209 Rat monoclonal [RMP1-30] to PDCD1 (Phycoerythrin) 100ug 3450
ab2621 Rabbit polyclonal to Histone H3 (tri methyl K79) - ChIP Grade 25ug 1380
ab2621 Rabbit polyclonal to Histone H3 (tri methyl K79) - ChIP Grade 100ug 3588
ab26211 Rat monoclonal [RMP1-30] to PDCD1 (Biotin) 100ug 3450
ab26212 Mouse monoclonal [10D7] to SSRP1 100ug 3450
ab26214 Rabbit polyclonal to Cdc25A 100ul 3450
ab26215 Rabbit polyclonal to DUSP20 100ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實驗中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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