植物維生素B12(VB12)ELISA 檢測(cè)試劑盒,植物維生素B12(VB12)ELISA 檢測(cè)試劑盒
植物維生素B12(VB12)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
植物維生素B12(VB12)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12408B 兔子神經(jīng)生長(zhǎng)因子(NGF)elisa試劑盒 rabbit Nerve growth factor,NGF Elisa試劑盒
RS12409B 兔子脫氫表雄酮硫酸酯(DHEA-S)elisa試劑盒 rabbit Dehydroepiandrosterone sulfate,DHEA-S Elisa試劑盒
RS12410B 兔子髓磷脂堿性蛋白(MBP)elisa試劑盒 Rabbit myelin basic protein,MBP Elisa試劑盒
RS12411B 兔子前列腺素E2(PGE2)elisa試劑盒 rabbit Prostaglandin E2,PG-E2 Elisa試劑盒
RS12412B 兔子前列腺素E1(PGE1)elisa試劑盒 rabbit Prostaglandin E1,PGE1 Elisa試劑盒
RS12413B 兔子皮質(zhì)酮/腎上腺酮(CORT)elisa試劑盒 rabbit Corticosterone,CORT Elisa試劑盒
RS12414B 兔子皮質(zhì)醇(Cortisol)elisa試劑盒 Rabbit Cortisol Elisa試劑盒
RS12415B 兔子腦鈉素/腦鈉尿肽(BNP)elisa試劑盒 rabbit brain natriuretic peptide,BNP Elisa試劑盒
RS12416B 兔子內(nèi)皮抑素(ES)elisa試劑盒 rabbit Endostatin,ES Elisa試劑盒
RS12417B 兔子內(nèi)皮素1(ET-1)elisa試劑盒 rabbit Endothelin 1,ET-1 Elisa試劑盒
RS12418B 兔子免疫球蛋白M(IgM)elisa試劑盒 rabbit Immunoglobulin M,IgM Elisa試劑盒
RS12419B 兔子免疫球蛋白G(IgG)elisa試劑盒 rabbit Immunoglobulin G,IgG Elisa試劑盒
RS12420B 兔子免疫球蛋白E(IgE)elisa試劑盒 rabbit Immunoglobulin E,IgE Elisa試劑盒
RS12421B 兔子免疫球蛋白A(IgA)elisa試劑盒 rabbit Immunoglobulin A,IgA Elisa試劑盒
RS12422B 兔子可溶性血管細(xì)胞粘附分子1(sVCAM-1)elisa試劑盒 Rabbit soluble vasccular cell adhesion molecule 1,sVCAM-1 Elisa試劑盒
RS12423B 兔子可溶性細(xì)胞間粘附分子1(sICAM-1)elisa試劑盒 rabbit Soluble InteFAellular Adhesion Molecule-1,sICAM-1 Elisa試劑盒
RS12424B 兔子可溶性E選擇素(sE-selectin)elisa試劑盒 rabbit soluble E-selectin,sE-selectin Elisa試劑盒
RS12425B 兔子巨噬細(xì)胞炎性蛋白5(MIP-5)elisa試劑盒 rabbit macrophage inflammatory protein 5,MIP-5 Elisa試劑盒
RS12426B 兔子堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)elisa試劑盒 rabbit basic fibroblast growth factor,bFGF Elisa試劑盒
RS12427B 兔子甲狀腺素(T4)elisa試劑盒 rabbit thyroxine,T4 Elisa試劑盒
RS12428B 兔子基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)elisa試劑盒 rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 Elisa試劑盒ab13620 Goat polyclonal to ZNF217 100ug 2768
ab13621 Mouse monoclonal [1051] to E. coli O157 50ug 3450
ab13622 Mouse monoclonal [1011] to E.coli 50ug 3450
ab13623 Mouse monoclonal [6911] to E. coli K99 pilus 50ug 3450
ab13624 Mouse monoclonal [1041] to E. coli O157 50ug 3450
ab13625 Mouse monoclonal [1031] to E. coli O157 50ug 3450
ab13626 Rabbit polyclonal to E.coli 500ul 3450
ab13627 Goat polyclonal to E.coli 500ul 3450
ab13628 Mouse monoclonal [IFR0111] to Salmonella 500ul 3450
ab13629 Mouse monoclonal [IFR0104] to Salmonella enteriditis 500ul 3450
ab1363 Goat polyclonal to DUSP8 100ug 3450
ab13630 Mouse monoclonal [6331] to Salmonella typhimurium 50ug 3450
ab13631 Mouse monoclonal [6341 ] to Salmonella LPS 50ug 3450
ab13633 Mouse monoclonal [6321] to Salmonella 50ug 3450
ab13634 Rabbit polyclonal to Salmonella 500ul 3450
ab13637 Mouse monoclonal [6311 ] to Salmonella typhimurium 50ug 3450
ab13638 Mouse monoclonal [IFR0402] to Salmonella typhimurium 500ul 3450
ab13639 Mouse monoclonal [6031] to Yersinia pestis 50ug 3450
ab13640 Rabbit polyclonal to Shigella 500ul 3450
ab13641 Mouse monoclonal [1b5d-1] to St. Louis encephalitis 50ug 3450
ab13642 Mouse monoclonal [6B5A-2] to St. Louis encephalitis 50ug 3450
ab13643 Mouse monoclonal [WD3C7] to Prion protein PrP 100ug 3450
ab13648 Rabbit polyclonal to MBD1 100ug 3450
ab13649 Mouse monoclonal [38C674] to DNp73 100ug 3450
ab1365 Goat polyclonal to MTMR1 100ug 2826
ab13650 Mouse monoclonal [46C713] to PDCD7 100ug 3450
ab13651 Mouse monoclonal [68C1039] to IL17E 100ug 3450
ab13652 Mouse monoclonal [73C175] to Chk2 100ug 3450
ab13653 Mouse monoclonal [97C691] to IL17B Receptor 100ug 3450
ab13654 Mouse monoclonal [114C307] to Noxa 100ug 3450
ab13655 Mouse monoclonal [95C657] to AKT2 200ul 3450
ab13656 Rabbit polyclonal to DAP Kinase 2 100ug 3450
ab13657 Rabbit polyclonal to INHAT1 200ul 3450
ab13658 Rabbit polyclonal to INHAT2 200ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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