豬血清一氧化氮(NO)ELISA 檢測(cè)試劑盒,豬血清一氧化氮(NO)ELISA 檢測(cè)試劑盒
豬血清一氧化氮(NO)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
豬血清一氧化氮(NO)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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RS11680B 大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒 Rat Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR Elisa試劑盒
RS11681B 大鼠白介素12(IL-12/P70)elisa試劑盒 Rat Interleukin 12,IL-12/P70 Elisa試劑盒
RS11682B 大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子B(VEGF-B)elisa試劑盒 Rat Vascular Endothelial cell Growth Factor B,VEGF-B Elisa試劑盒
RS11683B 大鼠白介素12(IL-12/P40)elisa試劑盒 Rat Interleukin 12,IL-12/P40 Elisa試劑盒
RS11684B 大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子C(VEGF-C)elisa試劑盒 Rat Vascular Endothelial cell Growth Factor C,VEGF-C Elisa試劑盒
RS11685B 大鼠白介素11(IL-11)elisa試劑盒 Rat Interleukin 11,IL-11 Elisa試劑盒
RS11686B 大鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白4(IGFBP-4)elisa試劑盒 Rat Insulin-like growth factor binding protein 4,IGFBP-4 Elisa試劑盒
RS11687B 大鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白3(IGFBP-3)elisa試劑盒 Rat Insulin-like growth factor binding protein 3,IGFBP-3 Elisa試劑盒
RS11688B 大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子D(VEGF-D)elisa試劑盒 Rat Vascular Endothelial cell Growth Factor D,VEGF-D Elisa試劑盒
RS11689B 大鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白2(IGFBP-2)elisa試劑盒 Rat Insulin-like growth factor binding protein 2,IGFBP-2 Elisa試劑盒
RS11690B 大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體1(VEGFR-1)elisa試劑盒 Rat Vascuoar endothelial cell growth factor receptor 1,VEGFR-1 Elisa試劑盒
RS11691B 大鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白1(IGFBP-1)elisa試劑盒 Rat Insulin-like growth factor binding protein 1,IGFBP-1 Elisa試劑盒
RS11692B 大鼠血管內(nèi)皮細(xì)胞生長(zhǎng)因子受體2(VEGFR-2)elisa試劑盒 Rat Vascuoar endothelial cell growth factor receptor 2,VEGFR-2 Elisa試劑盒
RS11693B 大鼠肝細(xì)胞生長(zhǎng)因子(HGF)elisa試劑盒 Rat hepatocyte growth factor,HGF Elisa試劑盒
RS11694B 大鼠生長(zhǎng)因子(GH)elisa試劑盒 Rat growth hormone,GH Elisa試劑盒
RS11695B 大鼠堿性成纖維細(xì)胞生長(zhǎng)因子9(bFGF-9)elisa試劑盒 Rat basic fibroblast growth factor 9,bFGF-9 Elisa試劑盒
RS11696B 大鼠堿性成纖維細(xì)胞生長(zhǎng)因子4(bFGF-4)elisa試劑盒 Rat basic fibroblast growth factor 4,bFGF-4 Elisa試劑盒
RS11697B 大鼠堿性成纖維細(xì)胞生長(zhǎng)因子6(bFGF-6)elisa試劑盒 Rat basic fibroblast growth factor 6,bFGF-6 Elisa試劑盒
RS11698B 大鼠酸性成纖維細(xì)胞生長(zhǎng)因子1(aFGF-1)elisa試劑盒 Rat acidic fibroblast growth factor 1,aFGF-1 Elisa試劑盒
RS11699B 大鼠凋亡相關(guān)因子配體(FASL) elisa試劑盒 Rat Factor-related Apoptosis ligand,FASL Elisa試劑盒
RS11700B 大鼠凋亡相關(guān)因子(FAS/CD95)elisa試劑盒 Rat Factor-related Apoptosis,FAS Elisa試劑盒
RS11701B 大鼠紅細(xì)胞生成素(EPO)elisa試劑盒 Rat Erythropoietin,EPO Elisa試劑盒 ab39037 Rabbit polyclonal to MMP20 - Carboxyterminal end 100ug 3450
ab39038 Rabbit polyclonal to MMP20 - Aminoterminal end 100ug 3450
ab39039 Rabbit polyclonal to MMP20 - Propeptide domain 100ug 3450
ab3904 Goat polyclonal to GPCR GPR2/CCR10 100ug 3450
ab39040 Rabbit polyclonal to MMP21 - Propeptide domain 100ug 3450
ab39041 Mouse monoclonal [6D4] to MHC Class 1 alpha + beta (Biotin) 100ug 3450
ab39045 Mouse monoclonal [6D4] to MHC Class 1 alpha + beta (Phycoerythrin) 100tests 4197
ab39047 Mouse monoclonal [MEM-262] to TCR V beta 5 (FITC) 100tests 3450
ab39048 Rat monoclonal [1B11] to CD43 (Allophycocyanin) 100ug 3450
ab39049 Rat monoclonal [1B11] to CD43 (Biotin) 200ug 4197
ab3905 Rabbit polyclonal to DOPA Decarboxylase 30ug 3450
ab39050 Rat monoclonal [1B11] to CD43 (FITC) 100ug 3450
ab39051 Rat monoclonal [1B11] to CD43 (Phycoerythrin) 100ug 3450
ab39057 Mouse monoclonal [10C4] to TCEB2 100ug 3450
ab39058 Mouse monoclonal [8A6] to NEK2 100ug 3450
ab3906 Rabbit polyclonal to DOPA Decarboxylase 30ug 3450
ab39068 Mouse monoclonal [2A3] to Plk1 (phospho T210) 100ug 3450
ab39069 Mouse monoclonal [6G2] to PRC1 100ug 3450
ab3907 Sheep polyclonal to Tryptophan Hydroxylase 50ul 3450
ab39072 GLP1R antibody 100ug 3450
ab39076 Rabbit polyclonal to MMP21 - Propeptide domain 100ug 3450
ab39077 Rabbit polyclonal to MMP21 - Aminoterminal end 100ug 3450
ab39078 Rabbit polyclonal to MMP21 - Aminoterminal end 100ug 3450
ab39079 Rabbit polyclonal to MMP21 - Hinge region 100ug 3450
ab3908 Rabbit polyclonal to CaMKII alpha 100ul 3450
ab39080 Rabbit polyclonal to MMP21 - Carboxyterminal end 100ug 3450
ab39082 Rabbit polyclonal to MMP22 - Propeptide domain 100ug 3450
ab39085 Rabbit polyclonal to MMP22 - Carboxyterminal end 100ug 3450
ab39086 Rabbit polyclonal to MMP23 - Hinge region 100ug 3450
ab39087 Rabbit polyclonal to MMP23 - Carboxyterminal end 100ug 3450
ab39088 Rabbit polyclonal to MMP23 - Aminoterminal end 100ug 3450
ab3909 Rabbit polyclonal to GAP43 (phospho S41) 100ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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