小鼠細(xì)胞間粘附分子3(ICAM-3/CD50)ELISA 檢測試劑盒,小鼠細(xì)胞間粘附分子3(ICAM-3/CD50)ELISA 檢測試劑盒
小鼠細(xì)胞間粘附分子3(ICAM-3/CD50)ELISA 檢測試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
小鼠細(xì)胞間粘附分子3(ICAM-3/CD50)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12926B 小鼠纖連蛋白(FN)elisa試劑盒 Mouse Fibronectin,FN Elisa試劑盒
RS12927B 小鼠凋亡相關(guān)因子配體(FASL)elisa試劑盒 Mouse Factor-related Apoptosis ligand,FASL Elisa試劑盒
RS12928B 小鼠凋亡相關(guān)因子(FAS/CD95)elisa試劑盒 Mouse Factor-related Apoptosis,FAS Elisa試劑盒
RS12929B 小鼠E選擇素(E-Selectin/CD62E)elisa試劑盒 Mouse E-Selectin Elisa試劑盒
RS12930B 小鼠紅細(xì)胞生成素(EPO)elisa試劑盒 Mouse Erythropoietin,EPO Elisa試劑盒
RS12931B 小鼠嗜酸粒細(xì)胞趨化蛋白Eotaxin 1(Eotaxin 1/CCL11)elisa試劑盒 Mouse Eotaxin 1 Elisa試劑盒
RS12932B 小鼠嗜酸粒細(xì)胞趨化因子(ECF)elisa試劑盒 Mouse eosinophil chemotactic factor,ECF Elisa試劑盒
RS12933B 小鼠可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)elisa試劑盒 Mouse soluble endothelial protein C receptor,sEPCR Elisa試劑盒
RS12934B 小鼠白細(xì)胞分化抗原30(CD30)elisa試劑盒 Mouse Cluster of differentiation 30,CD30 Elisa試劑盒
RS12935B 小鼠E鈣粘著蛋白/上皮性鈣黏附蛋白(E-Cad)elisa試劑盒 Mouse E-Cadherin,E-Cad Elisa試劑盒
RS12936B 小鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)elisa試劑盒 Mouse Bone morphogenetic protein receptor Ⅱ,BMPR-Ⅱ Elisa試劑盒
RS12937B 小鼠骨成型蛋白受體1A(BMPR-1A)elisa試劑盒 Mouse Bone morphogenetic protein receptor 1A,BMPR-1A Elisa試劑盒
RS12938B 小鼠骨成型蛋白4(BMP-4)elisa試劑盒 Mouse Bone morphogenetic protein 4,BMP-4 Elisa試劑盒
RS12939B 小鼠骨成型蛋白2(BMP-2)elisa試劑盒 Mouse Bone morphogenetic protein 2,BMP-2 Elisa試劑盒
RS12940B 小鼠腦源性神經(jīng)營養(yǎng)因子(BDNF)elisa試劑盒 Mouse Brain derived neurotrophic facor,BDNF Elisa試劑盒
RS12941B 小鼠活化素A(Activin-A)elisa試劑盒 Mouse Activin A Elisa試劑盒
RS12942B 小鼠角化細(xì)胞內(nèi)分泌因子(KAF)/雙調(diào)蛋白(AR)elisa試劑盒 Mouse keratinocyte autocrine factor/Amphiregulin,KAF/AR Elisa試劑盒
RS12943B 小鼠血管緊張素Ⅱ(ANG-Ⅱ)elisa試劑盒 Mouse angiotension Ⅱ,ANG-Ⅱ Elisa試劑盒
RS12944B 小鼠血管緊張素轉(zhuǎn)化酶(ACE)elisa試劑盒 Mouse Angiotensin converting enzyme,ACE Elisa試劑盒ab14734 Mouse monoclonal [20B12] to VDAC1 / Porin - Mitochondrial Loading Control 100ug 3450
ab14736 Goat polyclonal to BRD3 100ug 3450
ab14738 Mouse monoclonal [DR-4-02] to DR4 - Azide free 100ug 3450
ab14740 Mouse monoclonal [DR-6-04-EC] to DR6 100ug 3450
ab14743 Mouse monoclonal [SM2] to STAT1 100ug 3450
ab14744 Mouse monoclonal [20E8] to COX IV - Mitochondrial Loading Control 100ug 3738
ab14745 Mouse monoclonal [13G12] to UQCRC2 100ug 3450
ab14746 Mouse monoclonal [5A5] to UQCRFS1 100ug 3450
ab14747 Goat polyclonal to BS69 100ug 3450
ab14748 Mouse monoclonal [15H4] to ATP5A 100ug 3450
ab14749 Goat polyclonal to MID2 100ug 3450
ab14750 Rabbit polyclonal to R2D2 100ug 3450
ab14752 Goat polyclonal to SIGLECL1 100ug 2826
ab14755 Mouse monoclonal [13C3] to Carboxypeptidase B2 200ug 3450
ab14756 Mouse monoclonal [13D5] to Carboxypeptidase B2 200ug 3450
ab14757 Mouse monoclonal [13H4] to Carboxypeptidase B2 (HRP) 200ug 3450
ab14758 Goat polyclonal to FOXM1 100ug 3450
ab14759 Goat polyclonal to ETEA 100ug 3450
ab14760 Rabbit polyclonal to XTP4 100ug 3450
ab14761 Rabbit polyclonal to XTP4 100ug 3450
ab14762 Rabbit polyclonal to XTP4 100ug 3450
ab14763 Mouse monoclonal [15A10] to Carboxypeptidase B2 200ug 3450
ab14764 Goat polyclonal to BAF57/SMARCE1 100ug 3450
ab14765 Rabbit polyclonal to XTP4 100ug 3450
ab14766 Rabbit polyclonal to ErbB 2 100ug 3450
ab14767 Mouse monoclonal [19E2] to Carboxypeptidase B2 (HRP) 200ug 3450
ab14768 Rabbit polyclonal to E2F1 100ug 3450
ab14769 Rabbit polyclonal to E2F1 100ug 3450
ab14770 Rabbit polyclonal to E2F1 100ug 3450
ab14771 Mouse monoclonal [16C5] to Carboxypeptidase B2 200ug 3450
ab14772 Rabbit polyclonal to TdT 100ug 3450
ab14773 Rabbit polyclonal to SERHL 100ug 3450
ab14774 Rabbit polyclonal to SERHL 100ug 3450
ab14775 Rabbit polyclonal to SERHL 100ug 3450
ab14776 Rabbit polyclonal to ACK1 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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