小鼠巨噬細(xì)胞集落刺激因子(M-CSF)ELISA 檢測(cè)試劑盒,小鼠巨噬細(xì)胞集落刺激因子(M-CSF)ELISA 檢測(cè)試劑盒
小鼠巨噬細(xì)胞集落刺激因子(M-CSF)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
小鼠巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
ab2080 Goat polyclonal to alpha Synuclein 50ul 3450
ab20800 Mouse monoclonal [204] to Neutrophil Elastase 100ug 3450
ab20801 Rabbit polyclonal to Trypsin 100ul 3450
ab20803 Mouse monoclonal [HPRO-3] to Hydroxyprogesterone 100ug 3450
ab20805 Mouse monoclonal [716-281] to Papillomavirus Type 16 E7 Protein 500ug 3450
ab20808 Mouse monoclonal [CP37] to Ricinus communis agglutinin 500ug 3450
ab2081 Goat polyclonal to RanGAP1 100ug 3450
ab20810 Mouse monoclonal [B1B8.C2-Ky99] to MHC Class I H2 Kk (FITC) 250ug 3450
ab20814 Mouse monoclonal [3301] to Digoxin 500ug 3450
ab20815 Rabbit polyclonal to Neisseria gonorrhoeae (FITC) 100ul 3450
ab20818 Rabbit polyclonal to Legionella pneumophila (FITC) 100ul 3450
ab20821 Mouse monoclonal [804] to alpha 1 Antichymotrypsin 500ug 3450
ab20823 Goat polyclonal to HIV1 (Biotin) 100ul 3450
ab20825 Goat polyclonal to Apolipoprotein A II (HRP) 100ul 3450
ab20826 Mouse monoclonal [10D9H] to Salmonella O antigens 1mg 3450
ab20829 Rabbit polyclonal to Helicobacter pylori (FITC) 500ul 3450
ab2083 Goat polyclonal to Amyloid Precursor Protein 50ul 3450
ab20830 Mouse monoclonal [703] to alpha 1 Antitrypsin 100ug 3450
ab20832 Rabbit polyclonal to Mycobacterium 200ul 3450
ab20833 Mouse monoclonal [YPF19] to Yersinia pestis F1 antigen 500ug 3450
ab20836 Mouse monoclonal [711] to Rubella Virus HA antigen 100ug 3450
ab20837 Mouse monoclonal [104] to Factor VIII 100ul 3450
ab20839 Mouse monoclonal [1605] to Apolipoprotein B 100ug 3450
ab2084 Goat polyclonal to Amyloid Precursor Protein 50ul 3450
ab20840 Mouse monoclonal [811] to HSV1 gD 100ug 3450
ab20841 Goat polyclonal to Influenza A Virus 100ul 3450
ab20844 Rabbit polyclonal to Vaccinia Virus (HRP) 100ul 3450
ab20845 Mouse monoclonal [9161] to Neisseria gonorrhoeae 100ug 3450
ab20847 Mouse monoclonal [457] to Verotoxin 2 beta 100ug 3450
ab2085 Goat polyclonal to Amyloid Precursor Protein Frameshift Mutant 50ul 3450
ab20850 Mouse monoclonal [801] to Valproic Acid 100ul 3450
ab20851 Mouse monoclonal [4A1] to HIV1 p17 100ug 3450
ab20852 Mouse monoclonal [BDI167] to Hepatitis C Virus E2 100ug 3450 ab2080 Goat polyclonal to alpha Synuclein 50ul 3450
ab20800 Mouse monoclonal [204] to Neutrophil Elastase 100ug 3450
ab20801 Rabbit polyclonal to Trypsin 100ul 3450
ab20803 Mouse monoclonal [HPRO-3] to Hydroxyprogesterone 100ug 3450
ab20805 Mouse monoclonal [716-281] to Papillomavirus Type 16 E7 Protein 500ug 3450
ab20808 Mouse monoclonal [CP37] to Ricinus communis agglutinin 500ug 3450
ab2081 Goat polyclonal to RanGAP1 100ug 3450
ab20810 Mouse monoclonal [B1B8.C2-Ky99] to MHC Class I H2 Kk (FITC) 250ug 3450
ab20814 Mouse monoclonal [3301] to Digoxin 500ug 3450
ab20815 Rabbit polyclonal to Neisseria gonorrhoeae (FITC) 100ul 3450
ab20818 Rabbit polyclonal to Legionella pneumophila (FITC) 100ul 3450
ab20821 Mouse monoclonal [804] to alpha 1 Antichymotrypsin 500ug 3450
ab20823 Goat polyclonal to HIV1 (Biotin) 100ul 3450
ab20825 Goat polyclonal to Apolipoprotein A II (HRP) 100ul 3450
ab20826 Mouse monoclonal [10D9H] to Salmonella O antigens 1mg 3450
ab20829 Rabbit polyclonal to Helicobacter pylori (FITC) 500ul 3450
ab2083 Goat polyclonal to Amyloid Precursor Protein 50ul 3450
ab20830 Mouse monoclonal [703] to alpha 1 Antitrypsin 100ug 3450
ab20832 Rabbit polyclonal to Mycobacterium 200ul 3450
ab20833 Mouse monoclonal [YPF19] to Yersinia pestis F1 antigen 500ug 3450
ab20836 Mouse monoclonal [711] to Rubella Virus HA antigen 100ug 3450
ab20837 Mouse monoclonal [104] to Factor VIII 100ul 3450
ab20839 Mouse monoclonal [1605] to Apolipoprotein B 100ug 3450
ab2084 Goat polyclonal to Amyloid Precursor Protein 50ul 3450
ab20840 Mouse monoclonal [811] to HSV1 gD 100ug 3450
ab20841 Goat polyclonal to Influenza A Virus 100ul 3450
ab20844 Rabbit polyclonal to Vaccinia Virus (HRP) 100ul 3450
ab20845 Mouse monoclonal [9161] to Neisseria gonorrhoeae 100ug 3450
ab20847 Mouse monoclonal [457] to Verotoxin 2 beta 100ug 3450
ab2085 Goat polyclonal to Amyloid Precursor Protein Frameshift Mutant 50ul 3450
ab20850 Mouse monoclonal [801] to Valproic Acid 100ul 3450
ab20851 Mouse monoclonal [4A1] to HIV1 p17 100ug 3450
ab20852 Mouse monoclonal [BDI167] to Hepatitis C Virus E2 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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