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3-Methyladenine  (3-甲基腺嘌呤）

注：本產(chǎn)品僅用于科研，不可用于臨床

生物活性

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K

IC₅₀ & Target^[1]

體外研究
(In Vitro)

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner^[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions^[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy^[2].
3-Methyladenine induces cell death is independent of autophagy inhibition^[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest^[2].
Note:
在體外細(xì)胞培養(yǎng)實(shí)驗(yàn)中，3-MA 常用的濃度為 0.5-10 mM，所以優(yōu)先推薦直接稱取本次實(shí)驗(yàn)所需粉末量，用培養(yǎng)基全都溶解，0.22 μm 過濾器過濾除菌之后使用。建議溶液現(xiàn)配現(xiàn)用，盡快用完。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours^[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP^[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

149.15

性狀

Solid

Formula

C₆H₇N₅

CAS 號

5142-23-4

中文名稱

3-甲基腺嘌呤

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

溶解性數(shù)據(jù)