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產(chǎn)地	進(jìn)口	規(guī)格	100ul
級別	化工級	證書	ISO系列證書

高遷移率族蛋白1(HMGB1)多克隆抗體

Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)

HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

編號PAA399Mu01
物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
來源多抗制備
宿主兔
效價(jià)-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原RPA399Mu01-高遷移率族蛋白1(HMGB1)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500µg/mL
且適物種Homo sapiens (Human，人)
應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格20µl100µl 200µl 1ml 10ml
價(jià)格￥ 1361

特異性

該抗體是針對HMGB1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別HMGB1。

用法

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有明顯的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine	High mobility group box protein-1 (HMGB-1) as a new diagnostic marker in patients with acute appendicitis [PubMed: PMC3094252]
Respiration	Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice [Karger: 000319151]
Surgery	Clinical relevance of single nucleotide polymorphisms of the high mobility group box 1 protein gene in patients with major trauma in Southwest China [Pubmed: 22047946]
Viral Immunology	Is HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment? [Wiley: source]
Chinese Medical Journal	Severity of sepsis is correlated with the elevation of serum high-mobility group box 1 in rats [CMJ: 2009218808935901080]
Microbiology and Immunology	Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosis [PubMed: 23278480]
ACTA biochimica polonica	High mobility group B1 levels in sepsis and disseminated intravascular coagulation [Actabp: Source]
Anesthesia and Analgesia	The Inhibitory Effect of Lidocaine on the Release of High Mobility Group Box 1 in Lipopolysaccharide-Stimulated Macrophages [PubMed: 21288974]
Rheumatology International	Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of life [PubMed: 23143556]
Inflammation Research	Mammalian target of rapamycin complex 2 regulates inflammatory response to stress [PubMed: 22899279]
Acta Biochimica et Biophysica Sinica	IGF-1 alleviates ox-LDL-induced inflammation via reducing HMGB1 release in HAECs [PubMed: 22782142]
LabVest	The suppression of TRIM21 and the accumulation of IFN-α play crucial roles in the pathogenesis of osteonecrosis of the femoral head. [PubMed: 22825687]
Biomark Insights	Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
Evidence-based Complementary and Alternative Medicine	ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 Expression [PubMed: PMC3770047]
Exp Ther Med	Correlation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery disease [Pubmed: 23935732]
International of Rheumatic disesases	Serum levels of high mobility group box 1 protein and its association with quality of life and psychological and functional status in patients with fibromyalgia [Pubmed: 23901875]
Surgery	A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) [Pubmed: 24468034]
Acta Pharmacol Sin.	Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE-/- mice by downregulating the HMGB1-RAGE axis [Pubmed: 23564080]
BMC pulmonary medicine	Soluble receptor for advanced glycation end-products and progression of airway disease [Pubmed: 24758342]
Biochemical Pharmacology	Ascorbic acid reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and improves survival rate in septic mice by activation of Nrf2/HO-1 signals [Pubmed:25896849]
BioMed Research International	Effects of Single or Combined Treatments with Radiation and Chemotherapy on Survival and Danger Signals Expression in Glioblastoma Cell Lines [Hindawi:Source]
J Cardiothorac Surg.	High plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort study [Pubmed:Pmc4132233]
J Leukoc Biol.	HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells [Pubmed:24374967]
PLOS ONE	The Alarmin Concept Applied to Human Renal Transplantation: Evidence for a Differential Implication of HMGB1 and IL-33 [Pubmed:24586382]
Journal of Surgical Research	Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort study [Pubmed:25016440]