干擾素γ(IFNg)檢測試劑盒 返回列表頁

• 干擾素γ(IFNg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
  

• 編號SEA049Hu

• 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。

• 實驗方法雙抗夾心

• 反應時長3h

• 檢測范圍15.6-1,000pg/mL

• 靈敏度最小可檢測劑量小于等于6.1pg/mL.

• 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• 下載英文說明書   中文說明書

  
  

• 規(guī)格48T96T 96T*5 96T*10 96T*100

特異性

本試劑盒用于檢測干擾素γ(IFNg)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的干擾素γ(IFNg)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的干擾素γ(IFNg)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中干擾素γ(IFNg)含量的測定值與理論值的比率。

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

將干擾素γ(IFNg)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的干擾素γ(IFNg)與連接于固相載體上的抗體結合，然后加入生物su化的干擾素γ(IFNg)抗體，將未結合的生物su化抗體洗凈后，加入HRP標記的親和素，再次洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的干擾素γ(IFNg)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

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J Pharm Pharmacol	Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress [34244746]
J Nanobiotechnology	Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based “combo" nanoagent together with PD-L1 blockade in … [34225744]
Neoplasia	PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342]
Sci Rep	Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749]
Am J Transl Res	Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237]
J Immunol Res	Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633]
Sci Rep	Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology [34508139]
Transl Psychiatry	Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039]
Infect Drug Resist	Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic Lesions [34785914]
Environmental Research	In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and … [34863982]
Eur J Pharm Sci	Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment [34314841]

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