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細(xì)胞間粘附分子1(ICAM1)檢測試劑盒

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  • 型號

    SEA548Hu

  • 品牌

    云克隆

  • 廠商性質(zhì)

    代理商

  • 所在地

    杭州市

規(guī)格

48T 2092元 10000盒可售

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更新時(shí)間：2024-07-11 14:45:30瀏覽次數(shù)：153次

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產(chǎn)地 進(jìn)口 規(guī)格 48T
級別 化工級 證書 ISO系列證書
本試劑盒運(yùn)用雙抗體夾心 ELISA 法定量測定人血清、血漿、組織勻漿、細(xì)胞裂解液、細(xì)胞培養(yǎng)上清或其它相關(guān)生物液體中 ICAM1 含量。

細(xì)胞間粘附分子1(ICAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

  • 編號SEA548Hu

  • 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。

  • 實(shí)驗(yàn)方法雙抗夾心

  • 反應(yīng)時(shí)長3h

  • 檢測范圍78-5,000pg/mL

  • 靈敏度最小可檢測劑量小于等于34pg/mL.

  • 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

  • 下載英文說明書   中文說明書

  • 規(guī)格48T96T 96T*5 96T*10 96T*100




特異性

本試劑盒用于檢測細(xì)胞間粘附分子1(ICAM1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的細(xì)胞間粘附分子1(ICAM1)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)90-10496
EDTA plasma(n=5)79-9178
heparin plasma(n=5)78-10187

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的細(xì)胞間粘附分子1(ICAM1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中細(xì)胞間粘附分子1(ICAM1)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)94-105%98-105%94-102%84-95%
EDTA plasma(n=5)85-102%89-96%97-104%91-101%
heparin plasma(n=5)83-101%86-103%94-102%79-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將細(xì)胞間粘附分子1(ICAM1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的細(xì)胞間粘附分子1(ICAM1)與連接于固相載體上的抗體結(jié)合，然后加入生物su化的細(xì)胞間粘附分子1(ICAM1)抗體，將未結(jié)合的生物su化抗體洗凈后，加入HRP標(biāo)記的親和素，再次洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的細(xì)胞間粘附分子1(ICAM1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。

參考文獻(xiàn)

雜志參考文獻(xiàn)
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