Cyclin B1抗體說(shuō)明書,Anti
Catalog Number : RS-0572R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34（cdc2） to form the maturation-promoting factor （MPF）. Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]Subcellular Location : Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
Similarity : Belongs to the cyclin family. Cyclin AB subfamily.
Subunit : Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor （MPF）. The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization.
Specificity : Anti- Cyclin B1is a mouse polyclonal antibody
specific for Cyclin B1hu, rat, mo, cow
use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 48kDa
Cyclin B1抗體說(shuō)明書,AntiApplication :
– WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 lmmunohistochem1：100-500
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Cyclin B1抗體說(shuō)明書,Anti
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