說(shuō)明書(shū)下載EDLC.rar

分類(lèi)名稱(chēng) : 血液尿液化學(xué),代謝產(chǎn)物分析 產(chǎn)品介紹 :

測(cè)定方法：   比色法（565nm）定量測(cè)定D-乳酸的含量。

產(chǎn)品說(shuō)明：   乳酸是在缺氧或厭氧條件下由乳酸脫氫酶(LDH)催化生成。因此乳酸濃度的監(jiān)測(cè)是組織內(nèi)氧需求與利用平衡的良好指標(biāo)。同時(shí)
對(duì)細(xì)胞和動(dòng)物生理學(xué)研究也很重要。D-乳酸僅少量存在于動(dòng)物體內(nèi)并且對(duì)動(dòng)物樣品中D-乳酸的測(cè)量常作為評(píng)定細(xì)菌感染與否。
簡(jiǎn)單，直接和自動(dòng)化的乳酸濃度檢測(cè)方法是非?？扇〉?。博世生物技術(shù)提供的D-乳酸測(cè)試盒是基于乳酸脫氫酶催化氧化乳
酸，其中形成的NADH耦合到甲臜(MTT)發(fā)色體上。產(chǎn)物的顏色強(qiáng)度(565nm)與樣品中乳酸的濃度成正比。

產(chǎn)品特點(diǎn)：   敏感、準(zhǔn)確: 96-孔板檢測(cè)范圍為0.05～2 mM乳酸，對(duì)于含有酚紅的細(xì)胞培養(yǎng)物樣品，檢測(cè)范圍為0.1 ～1 mM 乳酸。

儲(chǔ)存條件：   所有試劑均于-20°C 保存。

DESCRIPTION   Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good  indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for Dlactate
in animal samples is a means to determine the presence of bacterial infection.

KEY FEATURES   Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples  containing phenol red: detection limit of 0.1 mM and linearity up to 1mM D-lactate in 96-well plate assay.

Storage conditions   Store all reagents at -20°C. Shelf life: 6 months after receipt.

其他 :

編號(hào)：EDLC-048, EDLC-100

測(cè)試：48T, 100T