說明書下載EACL.rar

分類名稱 : 血液尿液化學(xué),代謝產(chǎn)物分析 產(chǎn)品介紹 :

測(cè)定方法    比色法(570nm)或熒光法(530/585nm)定量測(cè)定乙酰膽堿的含量。

產(chǎn)品說明 乙酰膽堿是乙酰膽堿神經(jīng)元產(chǎn)生的一種神經(jīng)遞質(zhì)。它在骨骼肌的運(yùn)動(dòng)、平滑肌和心肌的調(diào)節(jié)、以及對(duì)學(xué)習(xí)、記憶和情緒都起著重要的作用。本公司生產(chǎn)的產(chǎn)品為測(cè)定生物樣品中的乙酰膽堿濃度提供了一種簡(jiǎn)便、直接、高通量的檢測(cè)方法。在該項(xiàng)檢測(cè)中，乙酰膽堿被乙酰膽堿脂酶水解成膽堿，而膽堿又被膽堿氧化酶氧化成甜菜堿和H₂O₂。生成的H₂O₂與一種特殊的顯色劑反應(yīng)形成一種粉紅色產(chǎn)物，其吸光強(qiáng)度（570nm）或熒光強(qiáng)度（530/585nm）直接與樣品中的乙酰膽堿濃度成正比。

產(chǎn)品特點(diǎn)    檢測(cè)范圍：比色法10-200 uM，熒光法0.4-10 uM乙酰膽堿。

產(chǎn)品用途    直接檢測(cè)生物樣品如血清、血漿、尿液、唾液、乳汁、組織和細(xì)胞培養(yǎng)物中的乙酰膽堿濃度; 藥物鑒定/藥理學(xué)：藥物對(duì)乙酰膽堿代謝的影響。

儲(chǔ)存條件： 冰運(yùn)。所有試劑均在-20°C下保存。

DESCRIPTION  ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. BioAssay Systems method provides a simple, direct and highthroughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.

APPLICATIONS  Assays: acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.  Drug Discovery/Pharmacology: effects of drugs on acetylcholine metabolism. 

KEY FEATURES   Linear detection range: colorimetric assay 10 to 200 uM, fluorimetric assay 0.4 to 10 uM acetylcholine.

STORAGE CONDITIONS  The kit is shipped on ice. Store all components at -20°C. Shelf life of three months after receipt.

編號(hào)：EACL-048, EACL-100

測(cè)試：48T, 100T