產(chǎn)品說明  β-葡萄糖苷酶是葡萄糖苷酶的一種，作用于連接兩個葡萄糖分子或葡萄糖替代分子（如纖維二糖）的β1->4鍵。許多生物體（如某些真菌，細(xì)菌和白蟻）都利用β-葡萄糖苷酶來消化纖維素。淚液中含有的溶菌酶是β-葡萄糖苷酶的一種， 其可阻止細(xì)菌感染眼部。在人類，異型β-葡萄糖苷酶（溶酶體葡糖腦苷酯酶）活性不足可能與高歇氏病和帕金森綜合癥有關(guān)。在科研和藥物研究中，方便、直接、自動化的β-葡萄糖苷酶活性檢測手段越來越受人們歡迎。本公司研制的β-葡萄糖苷酶檢測試劑盒直接用于生物樣本的檢測，免除了預(yù)處理過程。該改良測試方法利用底物試劑在β-葡萄糖苷酶作用下可水解生成黃色產(chǎn)物(zui大吸光率為405nm)。其反應(yīng)速度與酶的活性成正例。

產(chǎn)品特點  敏感度高、檢測范圍廣：采用20μL樣本，線性檢測范圍在2-250 U/L。操作簡便：僅用單一“混合-測量”步驟，可以在20分鐘內(nèi)準(zhǔn)確量化β-葡萄糖苷酶的活性。高通量：所有試劑均可用于高通量液體處理設(shè)備。

產(chǎn)品用途  1．直接檢測生物樣本中的α-葡萄糖苷酶活性。2．用于β-葡萄糖苷酶產(chǎn)品的鑒定與質(zhì)量控制。3．用于藥品研發(fā)中的高通量篩選以及對β-葡萄糖苷酶抑制劑效果的評估。

儲藏條件  可在室溫下運輸。所有試劑需在-20℃保存。

DESCRIPTION  β-GLUCOSIDASE is a glucosidase enzyme which acts upon β-1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal glucocerebrosidase) has been related to Gauchers disease and Parkinsons disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery. Our β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes a substrate that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

KEY FEATURES  High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments.

APPLICATIONS  1. Direct Assays: α-glucosidase activity in biological samples. 2. Characterization and Quality Control for α-glucosidase production. 3. Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.

STORAGE CONDITIONS  The kit is shipped at room temperature. Store all components at -20°C. Shelf life of at least 6 months.

編號：DBGD-048,DBGD-100

測試：48T,100T