說明書下載DAGD.rar

分類名稱 : 高效藥物篩選,酶活性測(cè)定 產(chǎn)品介紹 :

產(chǎn)品說明  α-葡萄糖苷酶可水解非還原性的1,4-α-D-葡萄糖殘基末端，并生成α-D-葡萄糖。動(dòng)物依靠α-葡萄糖苷酶將食物中的麥芽糖水解成葡萄糖后吸收。α-葡萄糖苷酶的活性異?？赡芘c糖尿病、龐佩氏癥等疾病有關(guān)。在科研和藥物研究中，方便、直接、自動(dòng)化的α-葡萄糖苷酶活性檢測(cè)手段越來越受人們歡迎。本公司研制的α-葡萄糖苷酶檢測(cè)試劑盒可直接用于檢測(cè)生物樣品中的α-葡萄糖苷酶活性，免除了預(yù)處理過程。該改良方法利用底物試劑被α-葡萄糖苷酶水解成黃色的產(chǎn)物, 其反應(yīng)速度與酶的活性成正比。

產(chǎn)品特點(diǎn)  敏感度高、檢測(cè)范圍廣：采用20μL樣本，線性檢測(cè)范圍在2-250 U/L。操作簡(jiǎn)便：僅用單一“混合-測(cè)量”步驟，可以在20分鐘內(nèi)準(zhǔn)確量化α-葡萄糖苷酶的活性。高通量：所有試劑均可用于高通量液體處理設(shè)備。

產(chǎn)品用途  1．直接檢測(cè)生物樣本中的α-葡萄糖苷酶活性。2．用于α-葡萄糖苷酶產(chǎn)品的鑒定與質(zhì)量控制。3．用于藥品研發(fā)中的高通量篩選以及對(duì)α-葡萄糖苷酶抑制劑效果的評(píng)估。

儲(chǔ)藏條件  可在室溫下運(yùn)輸。所有試劑需在-20℃保存。

DESCRIPTION  α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. Our α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The rate of the reaction is directly proportional to the enzyme activity.

KEY FEATURES  High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments.

APPLICATIONS  1. Direct Assays: α-glucosidase activity in biological samples. 2. Characterization and Quality Control for α-glucosidase production. 3. Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.

STORAGE CONDITIONS  The kit is shipped at room temperature. Store all components at -20°C. Shelf life of at least 6 months.

其他 :

編號(hào)：DAGD-048,DAGD-100

測(cè)試：48T,100T