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齊一生物小鼠肌鈣蛋白T(Tn-T)檢測試劑盒原裝Elisa試劑盒全國:,種類齊全,價格實惠,購買我公司產(chǎn)品的新老客戶,可享免費代測實驗服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報價,可我公司.
齊一生物小鼠肌鈣蛋白T(Tn-T)檢測試劑盒原實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標抗體的微孔中依次加入標本或標準品、生物素化的抗該指標抗體、HRP標記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成zui終的黃色.顏色的深淺和樣品中的該指標呈正相關(guān).用酶標儀在450nm波長下測定吸光度(OD值),計算樣品濃度.
原試劑盒組成及試劑配制
1. 酶聯(lián)板(Assay plate ):一塊(96孔).
2. 標準品(Standard):2瓶(凍干品).
3. 樣品稀釋液(Sample Diluent):1×20ml/瓶.
4. 生物素標記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根過氧化物酶標記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶.
6. 生物素標記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根過氧化物酶標記親和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 濃洗滌液(Wash Buffer):1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10. 終止液(Stop Solution):1×10ml/瓶(2N H2SO4).
10、原標本的采集及保存Mouse 60000SSA ELISA kit
Mouse Troponin T,Tn-T ELISA Kit
Mouse Leptospira IgG,Lep IgG ELISA Kit
Mouse C-Reactive Protein ELISA kit
Mouse Matrix Metalloproteinase-9,MMP-9 ELISA kit
Mouse matrix metalloproteinase 2Gelatinase A,MMP-2 ELISA kit
Mouse Osteopontin,OPN ELISA Kit
Mouse bovine serum albumin,BSA ELISA kit
Mouse interleukin 10,IL-10 ELISA KIT
Mouse Parathormone ,PTH ELISA kit
Mouse Vitamin D3,VD3 ELISA Kit
Mouse TT3 ELISA kit
Mouse Alanine aminotransferase,ALT ELISA Kit
Mouse Aspartate Aminotransferase ,AST ELISA kit
Mouse urokinase,UK Elisa Kit
Mouse Urea ELISA kit
Mouse Creatinine,Cr ELISA Kit
Mouse Vasoactive Intestinal Peptide,VIP ELISA Kit
Mouse OVA-sIgE ELISA kit
Mouse monoamine oxidase,MAO ELISA Kit
Mouse Xanthione ELISA kit
Mouse Chorionic Gonadotrophin,CG ELISA Kit
Mouse vascular endothelial growth factor ELISA kit
Mouse tissue inhibitors of metalloproteinase 1,TIMP-1 Elisa Kit
Mouse Transforming Growth factor β1,TGF-β1 ELISA kit
Mouse Noradrenaline,NE ELISA kit
Mouse Interleukin 23,IL-23 ELISA Kit
Mouse interleukin 17,IL-17 ELISA KIT
Mouse diacyl glycerol,DAG ELISA kit
Mouse secreted phospholipase A2,sPLA2 Elisa Kit
Mouse 50% plement hemolysis,CH50 ELISA Kit
Mouse eotaxin ELISA kit
Mouse muscarinic acetylcholine receptor M1,M-AChRM1 ELISA kit
Mouse muscarinic acetylcholine receptor M2,M-AChRM2 ELISA kit
Mouse mucin-5 subtype B,MUC5B ELISA kit
Mouse pituitary adenylate cyclase activating polypeptide,PACAP ELISA Kit
Mouse single stranded DNA Antibody,ssDNA ELISA Kit
Mouse phosphatidylserine,PS ELISA Kit
Mouse choline phosphoglyceride,PCCPG ELISA Kit