詳細介紹
運用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時對某個信號通路或疾病相關(guān)基因的多個基因的表達量變化進行檢測,芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對不同的信號通路和疾病相關(guān)基因設(shè)計了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號 | 名稱 | 基因列表 | 價格 |
啟因生物(wcgene.com) | HUMAN-WC094 | Small Molecule Transport qpcr array | SLC25A1, SLC25A10, SLC25A12, SLC25A13, SLC25A14, SLC25A15, SLC25A16, SLC25A17,SLC25A19, SLC25A2, SLC25A20, SLC25A21, SLC25A22, SLC25A23, SLC25A24, SLC25A25,SLC25A27, SLC25A3, SLC25A30, SLC25A31, SLC25A37, SLC25A4, SLC25A5. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC093 | Small Molecule Transport qpcr array | Slc25a1, Slc25a10, Slc25a12, Slc25a13, Slc25a14, Slc25a15, Slc25a16, Slc25a17,Slc25a19, Slc25a2, Slc25a20, Slc25a21, Slc25a22, Slc25a23, Slc25a24, Slc25a25,Slc25a27, Slc25a3, Slc25a30, Slc25a31, Slc25a37, Slc25a4, Slc25a5. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC095 | Small Molecule Transport qpcr array | Slc25a10, Slc25a12, Slc25a13, Slc25a14, Slc25a15, Slc25a16, Slc25a17, Slc25a19,Slc25a2, Slc25a20, Slc25a21, Slc25a22, Slc25a23, Slc25a24, Slc25a25, Slc25a27,Slc25a3, Slc25a30, Slc25a36, Slc25a37, Slc25a4, Slc25a5. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提取;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實時定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計算PCR芯片中的各個Ct值,采用ΔΔCt方法比較基因的表達變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進行比較。