TAMRA-Azide-Desthiobiotin

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (K_d = 10¹¹ vs. 10¹⁵M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Molecular weight	1145.33
Chemical composition	C57H82N11O14
CAS	N/A
Solubility	DMSO, DMF, DCM, THF, Chloroform
Purity	>95%
Appearance	Red amorphous solid to red oil
Storage conditions	-20C
Shipping conditions	Ambient temperature

Desthiobiotin是一種單環(huán)、無硫生物素的類似物，它與鏈霉菌素結(jié)合的特異性幾乎相同，但親和力卻不如生物素(Kd分別為1011和1015M)。因此，在與自由生物素競爭驅(qū)替的溫和條件下，可以很容易地、特別地從鏈霉毒素親和樹脂中分離出脫硫生物素。在生物樣品的下拉實驗中，desthiobiotin的軟釋放特性也有助于減少內(nèi)源性生物素化分子的共提純，這些分子在游離生物素的目標蛋白復合物的洗脫作用下仍與鏈球菌肽結(jié)合。

分子量	1145.33
分子式	C57H82N11O14
CAS	N/A
溶解度	DMSO, DMF, DCM, THF, Chloroform
含量	>95%
外觀	紅色無定形固體或紅油色有油質(zhì)
儲存	-20C
運輸	環(huán)境溫度

        西安凱新生物科技有限公司是國內(nèi)ling命科學和生物技術(shù)領域試劑供應商，從成立初期就致力于為生命科學和生物技術(shù)領域提供產(chǎn)品和技術(shù)服務，力求將的產(chǎn)品和服務奉獻給我們的客戶。目前主要產(chǎn)品有：合成磷脂、血清、高分子聚乙二醇衍生物、嵌段共聚物、磁性納米顆粒、納米金及納米金棒、近紅外熒光染料、活性熒光染料、熒光標記的葡聚糖BSA和鏈霉親和素、蛋白交聯(lián)劑、小分子PEG衍生物、點擊化學產(chǎn)品、樹枝狀聚合物、環(huán)糊精衍生物、熱門腫瘤原料藥、大環(huán)配體類、熒光量子點、透明質(zhì)酸衍生物、石墨烯或氧化石墨烯、碳納米管、富勒烯等等，可以滿足從毫克級到公斤級的定制需求。我們非常樂于傾聽您的需求，并一如既往地繼續(xù)為您提供的產(chǎn)品和服務。

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Click Chemistry Tool

DBCO Reagents

Amine Reactive

DBCO-C6-NHS Ester

• DBCO-NHS Ester
• DBCO-Sulfo-NHS Ester
• DBCO-PEG4-NHS Ester
• DBCO-PEG5-NHS Ester
• DBCO-PEG13-NHS Ester
• PC DBCO-NHS Ester
• DBCO-C6-Acid
• DBCO-Acid
• DBCO-PEG5-Acid

Carboxyl/Carbonyl Reactive

• DBCO-Amine
• DBCO-PEG4-Amine
• Sulfo DBCO-PEG4-Amine
• Sulfo DBCO-Amine

 Sulfhydryl Reactive

• Sulfo DBCO-Maleimide

DBCO-Maleimide

• Sulfo DBCO-PEG4-Maleimide
• DBCO-PEG4-Maleimide
• Bis-sulfone-PEG4-DBCO
• ThioLinker-DBCO

Fluorescent Probes

• MB 532 DBCO

MB 488 DBCO

• MB 543 DBCO
• MB 594 DBCO
• Carboxyrhodamine 110 DBCO
• TAMRA DBCO
• Cy3 DBCO
• Cy5 DBCO
• Cy5.5 DBCO
• Cy7 DBCO

 Biotinylation Reagents

• DBCO-PEG4-Biotin

WS DBCO-Biotin

• DBCO-PEG4-Desthiobiotin
• Dde Biotin-DBCO
• PC DBCO-Biotin
• WS PC DBCO-Biotin
• Diazo Biotin-DBCO

PEGylation Reagents

• DBCO-mPEG, 5 kDa

DBCO-mPEG, 10 kDa

• DBCO-mPEG, 20 kDa
• DBCO-mPEG, 30 kDa

 Bifunctional Crosslinkers

• Methyltetrazine-DBCO

Methyltetrazine-PEG12-DBCO

• DBCO-PEG4-DBCO
• TCO-PEG12-DBCO

 Miscellaneous Reagents

• DBCO-PEG4-Hydroxyl