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ThioLinker-DBCO
西安凱新生物科技有限公司是國(guó)內(nèi)專業(yè)的點(diǎn)擊化學(xué)試劑供應(yīng)商，產(chǎn)品廣泛應(yīng)用于各大科研院校、檢驗(yàn)機(jī)構(gòu)、環(huán)境監(jiān)測(cè)、生物制藥、石油化工、航天技術(shù)、公共安全等眾多領(lǐng)域。我公司提供的點(diǎn)擊類化學(xué)試劑包括：DBCO、TCO、Tetrazine、Azide、Alkyne、Auxiliary reagents等。其中DBCO Reagents又包含了與氨基反應(yīng)的，與羧基反應(yīng)的，與生物素交聯(lián)的，與熒光素交聯(lián)的，與PEG交聯(lián)的等等產(chǎn)品。各試劑具有多樣的規(guī)格和齊全的包裝，能滿足您各類實(shí)驗(yàn)需求，西安凱新所有員工希望能與您攜手共進(jìn)，共創(chuàng)輝煌。
公司：西安凱新生物科技有限公司
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銷售：.
ThioLinker-DBCO,Click Chemistry-DBCO Reagents:
Molecular weight | 1140.39 |
Molecular formula | C59H69N3O14S3 |
CAS | N/A |
Solubility | Water, DMSO, DMF |
Purity | >90% (HPLC) |
Appearance | White to grey amorphous solid |
Storage Conditions | -20°C |
Shipping conditions | Ambient temperature |
ThioLinker-DBCO結(jié)構(gòu)式
Click Chemistry產(chǎn)品分類：
DBCO Reagents
Amine Reactive
Carboxyl/Carbonyl Reactive
Sulfhydryl Reactive
Bifunctional Crosslinkers
Biotinylation Reagents
Fluorescent Probes
PEGylation Reagents
Miscellaneous Reagents
TCO Reagents
Amine Reactive
Sulfhydryl Reactive
Miscellaneous Reagents
Tetrazine Reagents
Amine Reactive
Carboxyl/Carbonyl Reactive
Sulfhydryl Reactive
PEGylation Reagents
Fluorescent Probes
Bifunctional Crosslinkers
Azide Reagents
Amine Reactive
Carboxyl/Carbonyl Reactive
Sulfhydryl Reactive
Biotinylation Reagents
Fluorescent Azides
Alkyne Reagents
Labeling Reagents
Biotinylation Reagents
Fluorescent Alkynes
DBCO Reagents-Amine Reactive產(chǎn)品信息包括以下：
DBCO-NHS Ester MW：402.40
Photocleavable DBCO-NHS ester MW:945.96
DBCO-C6-NHS Ester MW:430.45
DBCO-PEG5-NHS Ester MW:693.74
DBCO-PEG4-NHS Ester MW:649.68
DBCO-Sulfo-NHS Ester MW:532.50
DBCO-Acid MW:305.11
DBCO-C6-Acid MW:333.83
DBCO-PEG5-Acid MW:596.67
DBCO-PEG4-Acid MW:552.62
DBCO-PEG4-4-Formylbenzoate MW:655.77
DBCO Reagents-Carboxyl/Carbonyl Reactive產(chǎn)品信息包括以下：
DBCO-Amine MW:276.33 CAS:1255942-06-3
DBCO-PEG4-Amine MW:523.62
DBCO Reagents-Sulfhydryl Reactive產(chǎn)品信息包括以下：
ThioLinker-DBCO MW:1140.39
DBCO-Maleimide MW:427.75
DBCO-PEG4-Maleimide MW:674.74
Bis-sulfone-PEG4-DBCO MW：1006.19
Bis-sulfone-PEG3-Azide MW:700.82
Bis-sulfone-PEG4-Tetrazine
DBCO Reagents-Bifunctional Crosslinkers產(chǎn)品信息包括以下：
DBCO-PEG4-DBCO MW:854.92
TCO-PEG12-DBCO
Methyltetrazine-DBCO
Tetrazine-PEG12-DBCO
DBCO-PEG4-4-Formylbenzoate
DBCO Reagents-Biotinylation Reagents產(chǎn)品信息包括以下：
DBCO-PEG4-Biotin
WS DBCO-Biotin
photoclevable DBCO-Biotin
DBCO-PEG4-Desthiobiotin
WS-PC DBCO-Biotin
Dde Biotin-DBCO
Diazo Biotin-DBCO
DBCO-S-S-PEG3-Biotin
DBCO Reagents-Fluorescent Probes產(chǎn)品信息包括以下：
Carboxyrhodamine 110 DBCO
Sulforhodamine B DBCO
TAMRA DBCO
Sulforhodamine 101 DBCO
Cy3 DBCO
Cy5 DBCO
Cy5.5 DBCO
Cy7 DBCO
DBCO Reagents-PEGylation Reagents產(chǎn)品信息包括以下：
DBCO-mPEG, 5 kDa
DBCO-mPEG, 10 kDa
DBCO-mPEG, 20 kDa
DBCO-mPEG, 30 kDa
DBCO Reagents-Miscellaneous Reagents產(chǎn)品信息包括以下：
DBCO-PEG4-Phosphoramidite
DBCO-PEG4-Hydroxyl
TCO Reagents-Amine Reactive產(chǎn)品信息包括以下：
TCO-NHS Ester
TCO-PEG4-NHS Ester
TCO-PEG12-NHS Ester
UV-Tracer TCO-NHS Ester
TCO-PEG4-Acid
TCO Reagents- Sulfhydryl Reactive產(chǎn)品信息包括以下：
TCO-PEG3-Maleimide
TCO Reagents- Miscellaneous Reagents產(chǎn)品信息包括以下：
TCO-Amine HCl Salt
TCO-PEG12-DBCO
Cy5-TCO
Tetrazine Reagents-Amine Reactive產(chǎn)品信息包括以下：
Tetrazine-PEG5-NHS Ester
photocleavable-Methyltetrazine-NHS Ester
Methyltetrazine-PEG4-NHS Ester
Methyltetrazine-Sulfo-NHS Ester
Methyltetrazine-PEG4-Acid
Methyltetrazine-PEG12-NHS Ester
Methyltetrazine-NHS Ester
Methyltetrazine-Acid
Tetrazine-Acid
Tetrazine Reagents- Carboxyl/Carbonyl Reactive產(chǎn)品信息包括以下：
Tetrazine-Amine
Methyltetrazine-Amine
Methyltetrazine-Propylamine
Methyltetrazine-PEG4-Amine
Tetrazine Reagents- Sulfhydryl Reactive產(chǎn)品信息包括以下：
Methyltetrazine-PEG4-Maleimide
ThioLinker-Tetrazine
Tetrazine Reagents- PEGylation Reagents產(chǎn)品信息包括以下：
Tetrazine-mPEG, 5 kDa
Tetrazine-mPEG, 10 kDa
Tetrazine-mPEG, 20 kDa
Tetrazine-mPEG, 30 kDa
Tetrazine Reagents- Fluorescent Probes產(chǎn)品信息包括以下：
Cy3 Tetrazine
Cy5 Tetrazine
Cy7 Methyltetrazine
TAMRA Methyltetrazine
Tetrazine Reagents- Bifunctional Crosslinkers產(chǎn)品信息包括以下：
Tetrazine-PEG4-Alkyne
Methyltetrazine-DBCO
Tetrazine-PEG12-DBCO
Tetrazine-PEG4-Azide
Azide Reagents-Amine Reactive產(chǎn)品信息包括以下：
Azido-PEG4-NHS Ester
photocleavable-Azido-NHS Ester
UV-Tracer Azido-NHS Ester
Azidoacetic Acid NHS Ester
Azido-PEG4-Acid
Azide Reagents-Carboxyl/Carbonyl Reactive產(chǎn)品信息包括以下：
Azido-Propylamine
Azido-PEG3-Amine
Azide Reagents-Sulfhydryl Reactive產(chǎn)品信息包括以下：
Azido-PEG3-Maleimide
ThioLinker-Azide
Azide Reagents-Biotinylation Reagents產(chǎn)品信息包括以下：
Biotin-PEG3-Azide
photoclevable-Biotin-Azide
TAMRA-Azide-Biotin
Desthiobiotin-PEG3-Azide
TAMRA-Azide-Desthiobiotin
Dde Biotin-Azide
Diol Biotin-Azide
Diazo Biotin-Azide
Azide Reagents-Fluorescent Azides產(chǎn)品信息包括以下：
Carboxyrhodamine 110 Azide
TAMRA Azide
Sulforhodamine B Azide
Sulforhodamine 101 Azide
Cy3 Azide
Cy5 Azide
Cy5.5 Azide
Cy7 Azide
Alkyne Reagents-Amine Reactive產(chǎn)品信息包括以下：
Alkyne-PEG4-NHS Ester
photocleavable-Alkyne-NHS Ester
Propargyl-NHS Ester
Alkyne-PEG4-Acid
Alkyne-PEG4-Amine
Alkyne-PEG4-Maleimide
Propargyl-Maleimide
Alkyne Reagents-Biotinylation Reagents產(chǎn)品信息包括以下：
Biotin-PEG4-Alkyne
Desthiobiotin-PEG4-Alkyne
photoclevable-Biotin-Alkyne
Dde Biotin-Alkyne
Diol Biotin-Alkyne
Diazo Biotin-Alkyne
Alkyne Reagents- Fluorescent Alkynes產(chǎn)品信息包括以下：
Carboxyrhodamine 110 Alkyne
Sulforhodamine B Alkyne
Sulforhodamine 101 Alkyne
Cy3 Alkyne
TAMRA Alkyne
Cy5 Alkyne
Cy5.5 Alkyne
Cy7 Alkyne
名稱：DBCO-NHS Ester MW：402.40
英文介紹：
DBCO-NHS Ester reacts with primary amines
This product is not remended for labeling of proteins or any otherbiopolymers in aueous buffers due to poor aueous solubility.
名稱：Photocleavable DBCO-NHS ester MW:945.96
英文介紹：
An amine-reactive, photocleavable labeling reagent.
Photocleavable DBCO-NHS ester reagent contains an amine reactive grouplinked to DBCO moiety through a spacer arm containing a photocleavablemoiety. Molecules jointed by the copper-free click reaction can beefficiently photoreleased, typically >90% in 5-25 minutes using aninexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).
名稱: DBCO-C6-NHS Ester MW:430.45
英文介紹：
An activated ester with extended 6-carbon atom long spacer.
DBCO-NHS Ester reacts with primary amines (e.g., side chain of lysineresidues or aminosilane-coated surfaces) at pH 7-9 to form covalentbonds. Extended 6-carbon spacer arm is beneficial for some application.This reagent has limited shelf life; however, corresponding acid and itsderivatives are found to be stable over long period of time.DBCO-Sulfo-NHS-Ester, a water-soluble version of this product is alsoavailable for labeling experiments wher the presence of organicsolvents is highly undesirable.
名稱：DBCO-PEG5-NHS Ester MW:693.74
英文簡(jiǎn)介：
An amine-reactive labeling reagent with enhanced water stability and solubility.
DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primaryamine (e.g., side chain of lysine residues or aminosilane-coatedsurfaces) at pH 7-9 to form a covalent bond. The hydrophilicpolyethylene glycol (PEG) spacer arm imparts water solubility that istransferred to the labeled molecule, thus reducing aggregation oflabeled proteins stored in solution. The PEG spacer arm provides a longand flexible connection that minimizes steric hindrance involved withligation to plementary azide-containing molecules.
名稱：DBCO-PEG4-NHS Ester MW:649.68
英文簡(jiǎn)介：
An amine-reactive labeling reagent with enhanced water solubilityprovided by a PEG4 spacer.
DBCO-PEG4-NHS Ester reacts specifically and efficiently with a primaryamine (e.g., side chain of lysine residues or aminosilane-coatedsurfaces) at pH 7-9 to form a covalent bond. The hydrophilicpolyethylene glycol (PEG) spacer arm imparts water solubility that istransferred to the labeled molecule, thus reducing aggregation oflabeled proteins stored in solution. The PEG spacer arm provides a longand flexible connection that minimizes steric hindrance involved withligation to plementary azide-containing molecules.
名稱：DBCO-Sulfo-NHS Ester MW:532.50
英文簡(jiǎn)介：
A water-soluble reagent for labeling antibodies, proteins and othermolecules containing primary amines.
DBCO-Sulfo-NHS-Ester enables simple and efficient labeling ofantibodies, proteins and any other primary amine-containingmacromolecules with DBCO moiety. This reagent is water-soluble, enablinglabeling to be performed in the absence of organic solvents such as DMSOor DMF for applications that cannot tolerate solvents or are plicatedby their inclusion. Specific labeling of cell surface proteins isanother mon application for these uniuely water-soluble and membraneimpermeable reagents.
名稱：DBCO-Acid MW:305.11
英文簡(jiǎn)介：
DBCO-Acid is an amine reactive, non-activated cyclooctyne building block.
The use of strained cyclooctynes instead of terminal alkynes allows forovering a major drawback of copper-catalyzed azide-alkynecycloaddition (CuAAC) - the need for the toxic copper catalyst. The ringstrain in cyclooctynes, such as DBCO-Acid makes them highly reactive inthe cycloaddition reaction. This reaction was termed as thestrain-promoted alkyne-azide cycloaddition (SPAAC) or copper-free clickreaction and has been widely used for bioconjugation and for labelingliving cells and living animals.
名稱：DBCO-C6-Acid MW:333.83
英文簡(jiǎn)介：
An analog of DBCO-Acid with improved stability and solubility inmonly used organic solvents.
DBCO reagents, also known as ADIBO or DIBAC are routinely used buildingblocks for synthesis of plex molecules to be used in Cu-free ligation applications.
Using DBCO-C6-Acid primary or secondary amines can be easilyfunctionalized with DBCO moiety in the presence of activators (e.g. EDC,or HATU) through a stable amide bond. A longer carbon spacer armimproves stability and solubility of this reagent in monly usedorganic solvents.
名稱：DBCO-PEG5-Acid MW:596.67
英文簡(jiǎn)介：
An amine-reactive building block with enhanced stability and solubilityin aueous media.
DBCO-PEG5-Acid is a polyethylene glycol-containing reagent withterminal carboxylic acid (–COOH) that can be reacted with primary orsecondary amine groups in the presence activators (e.g. EDC) forming astable amide bond. The PEG spacer arm is hydrophilic and providesgreater solubility to labeled molecules pared to reagents having onlyhydrocarbon spacers. The PEG spacer arm also gives the reagent a longand flexible connection to minimize steric hindrance involved withreacting with plementary azido-modified molecules.
名稱：DBCO-PEG4-Acid MW:552.62
英文簡(jiǎn)介：
An amine-reactive building block with enhanced solubility in aueous media.
DBCO-PEG4-Acid is a polyethylene glycol-containing reagent withterminal carboxylic acid (–COOH) that can be reacted with primary orsecondary amine groups in the presence activators (e.g. EDC) forming astable amide bond. The PEG spacer arm is hydrophilic and providesgreater solubility to labeled molecules pared to reagents having onlyhydrocarbon spacers
名稱：DBCO-PEG4-4-Formylbenzoate MW:655.77
英文簡(jiǎn)介：
A heterobifunctional reagent for labeling aminooxy-containing pounds.
DBCO-PEG4-4-Formylbenzoate reacts specifically and efficiently with anaminooxy group at pH 4-5 to form a covalent oxime bond. The oximeligation with aromatic aldehydes is highly chemoselecive and ispatible with other functional groups present in peptides orbiomolecules. An aniline catalysis can be used to activate aromaticaldehyde towards an aminooxy group and greatly enhance the rate ofligation reaction.
DBCO Reagents-Carboxyl/Carbonyl Reactive產(chǎn)品詳細(xì)英文介紹和使用說(shuō)明：
名稱：DBCO-Amine MW:276.33 CAS:1255942-06-3
英文簡(jiǎn)介：
A carboxyl-reactive building block containing DBCO moiety and aterminal primary amine group.
DBCO-Amine is a simple DBCO-containing reagent possessing a terminalprimary amines group (–NH2) that reacts with carboxyl groups in thepresence of activators (e.g. EDC, or DCC) or with activated esters (e.g.NHS esters) forming stable amide bonds. A short spacer arm adds minimalmass to modified molecules (273.3 daltons).
名稱：DBCO-PEG4-Amine MW:523.62
英文簡(jiǎn)介：
A carbonyl-reactive building block with enhanced solubility in aueous media.
DBCO-PEG4-Amine is a polyethylene glycol-containing reagent withterminal primary amines (–NH2) that reacts with carboxyl groups in thepresence of activators (e.g. EDC, or DCC) or with activated esters (e.g.NHS esters) forming stable amide bonds. A hydrophilic spacer armenhances solubility in aueous buffers.
名稱：Thiolinker-DBCO
英文簡(jiǎn)介：
A uniue, water-soluble, bis-alkylating reagent that allows forsite-specific labeling of antibodies under mild aueous conditions.
The traditional sulfhydryl labeling chemistry cleaves disulfide bridgesand leaves them in &lduo;open form&rduo;, which may promise the stability andfunction of an antibody or other proteins. ThiolinkerTM-DCBO re-bridgesthe disulfide bond, and enhances the structural stability of theprotein. In addition, conjugates prepared with ThiolinkerTM reagentsbenefits from reduced heterogeneity and better stability.
名稱：DBCO-Maleimide MW:427.75
英文簡(jiǎn)介：
A short-length, sulfhydryl-reactive labeling reagent that contains ashort hydrocarbon spacer.
DBCO-Maleimide enables simple and efficient incorporation of DBoiety onto cysteine-containing peptides or other thiol-containingmolecules. The maleimide group specifically and efficiently reacts withreduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stablethioether bonds. A short spacer arm adds minimal mass to modifiedmolecules (427.4 daltons).
名稱：DBCO-PEG4-Maleimide MW:674.74
英文簡(jiǎn)介：
A mid-length, sulfhydryl-reactive labeling reagent with enhanced watersolubility provided by a PEG4 spacer.
DBCO-PEG4-Maleimide enables simple and efficient incorporation of DBoiety onto antibodies, cysteine-containing peptides and otherthiol-containing molecules. The hydrophilic polyethylene glycol (PEG)spacer arm imparts water solubility that is transferred to the labeledmolecule, thus reducing aggregation of labeled proteins stored in thesolution. The PEG spacer arm provides a long and flexible connectionthat minimizes steric hindrance involved with ligation to plementaryazide-containing molecules.
名稱：Bis-sulfone-PEG4-DBCO MW：1006.19
英文簡(jiǎn)介：
A bis-alkylating labeling reagent used to create conjugates withenhanced structural stability.
Bis-sulfone-PEG4-DBCO enables site-specific incorporation of DBoiety onto antibodies to form well defined conjugates. Unlikeconjugates prepared through conventional, maleimide-based labelingchemistry that leaves disulfide bridges in &lduo;open form&rduo; and produces aheterogeneous mixture labeled species with DAR 1-8, conjugates preparedwith bis-sulfone reagents benefits from reduced heterogeneity andenhanced stability.
名稱：Bis-sulfone-PEG3-Azide MW:700.82
英文簡(jiǎn)介：
A bis-alkylating labeling reagent used to create well-definedconjugates with enhanced structural stability.
Bis-sulfone-PEG3-Azide enables site-specific incorporation of an azidegroup onto antibodies to form well defined conjugates. Unlike conjugatesprepared through conventional, maleimide-based labeling chemistry thatleaves disulfide bridges in &lduo;open form&rduo; and produces a heterogeneousmixture labeled species with DAR 1-8, conjugates prepared withbis-sulfone reagents benefits from reduced heterogeneity and enhanced stability.
DBCO Reagents-Bifunctional Crosslinkers產(chǎn)品詳細(xì)英文介紹和使用說(shuō)明：
名稱：DBCO-PEG4-DBCO MW:854.92
英文簡(jiǎn)介：
An azide-to-azide homobifunctional cross-linker with a hydrophilic PEG4spacer arm.
DBCO-PEG4-DBCO reacts specifically and efficiently with an azide group(e.g., a side chain of L-azidohomoalanine) to form a stable triazole moiety.
名稱：TCO-PEG12-DBCO
英文簡(jiǎn)介：
An azide-to-tetrazine heterobifunctional cross-linker with ahydrophilic PEG12 spacer arm.
Heterobifunctional reagents are used for converting azido-containingbiomolecules into TCO-modified biomolecules. TCO-PEG12-DBCO can serve asa heterobifunctional azide-to-tetrazine cross-linker for covalentlylinking azido- and tetrazine-modified peptides or biopolymers. The PEG12spacer arm provides a long and flexible connection that minimizes sterichindrance involved with ligation to plementary reagent.
名稱：Methyltetrazine-DBCO
英文簡(jiǎn)介：
A water-soluble azide-to-TCO heterobifunctional crosslinker.
Heterobifunctional reagents used for converting azido-containingpeptides or protein into tetrazine-modified peptides or protein withoutthe need for catalyst or axillary reagents. Tetrazine-DBCO can serve asa heterobifunctional azide-to-TCO cross-linker for covalently linkingazido- and TCO-modified peptides or biopolymers.
名稱：Tetrazine-PEG12-DBCO
英文簡(jiǎn)介：
An azide-to-TCO heterobifunctional crosslinker with a hydrophilic PEG12spacer arm.
Heterobifunctional reagents used for converting azido-containingpeptides or protein into tetrazine-modified peptides or protein withoutthe need for catalyst or axillary reagents. Tetrazine-PEG12-DBCO canserve as a heterobifunctional azide-to-TCO cross-linker for covalentlylinking azido- and TCO-modified peptides or biopolymers.
名稱：DBCO-PEG4-4-Formylbenzoate
英文簡(jiǎn)介：
A heterobifunctional reagent for labeling aminooxy-containing pounds.
DBCO-PEG4-4-Formylbenzoate reacts specifically and efficiently with anaminooxy group at pH 4-5 to form a covalent oxime bond. The oximeligation with aromatic aldehydes is highly chemoselecive and ispatible with other functional groups present in peptides orbiomolecules. An aniline catalysis can be used to activate aromaticaldehyde towards an aminooxy group and greatly enhance the rate ofligation reaction.
名稱：DBCO-PEG4-Biotin
英文簡(jiǎn)介：
An azide-reactive biotinylation reagent with an extended PEG4 spacer arm.
DBCO reacts with an azide to produce a stable triazole, also referredto as the Cu(I)‐free or strain-promoted click reaction. The azide group,which is the reactive target of DBCO-activated biotin, can be introducedinto proteins or other cellular targets via azide labeling reagents orthrough in vivo labeling with azide-derivatives of naturally occurringmetabolic building blocks. This reaction possesses extreme selectivityand biopatibility, such that the plimentary reagents can formcovalent bonds within richly functionalized biological systems, and, insome cases, living organisms.
名稱：WS DBCO-Biotin
英文簡(jiǎn)介：
A water-soluble, azide-reactive biotinylation reagent.
DBCO reacts with an azide to produce a stable triazole, also referredto as the Cu(I)‐free or strain-promoted click reaction. The azide group,which is the reactive target of DBCO-activated biotin, can be introducedinto proteins or other cellular targets via azide labeling reagents orthrough in vivo labeling with azide-derivatives of naturally occurringmetabolic building blocks. This reaction is extremely selective andbiopatible, such that the plimentary reagents can form a covalentbond within richly functionalized biological systems, and, in somecases, living organisms.
名稱：photoclevable DBCO-Biotin
英文簡(jiǎn)介：
An azide-reactive, photoclevable biotin probe that allows forreagent-free release of the captured biomolecules fromstreptavidin.Extraordinary strength of the streptavidin-biotininteraction allows for efficient capturing of even highly dilutetargets; however, it makes recovery of proteins from affinity resins challenging.
名稱：DBCO-PEG4-Desthiobiotin
英文簡(jiǎn)介：
An azide-reactive desthiobiotin reagent that allows easy elution from streptavidin.
Desthiobiotin is a single-ring, sulfur-free analog of biotin that bindsto streptavidin with nearly eual specificity but less affinity thanbiotin (Kd = 1011 vs. 1015M, respectively). Conseuently,desthiobiotinylated proteins can be eluted readily and specifically fromstreptavidin affinity resin using mild conditions based on petitivedisplacement with free biotin. For pull-down experiments with biologicalsamples, this soft-release characteristic of desthiobiotin also helps tominimize co-purification of endogenous biotinylated molecules, whichremain bound to streptavidin upon elution of the target proteinplexes with free biotin.
名稱：WS-PC DBCO-Biotin
英文簡(jiǎn)介：
A water-soluble, photoclevable biotin probe that allows forreagent-free release of the captured biomolecules from streptavidin.
Extraordinary strength of the streptavidin-biotin interaction allowsfor efficient capturing of even highly dilute targets; however, it makesrecovery of proteins from affinity resins challenging. Photocleavablebiotin probes eliminate a major limitation of the streptavidin-biotinaffinity purification. This reagent contains a biotin moiety linked toan azide moiety through a spacer arm containing a photocleavable moiety.Captured biomolecules can be efficiently photoreleased, typically>90% in 5-25 minutes using an inexpensive, near-UV, low intensitylamp (e.g. 365 nm lamp at 1-5 mW/cm2).
名稱：Dde Biotin-DBCO
英文簡(jiǎn)介：
A hydrazine cleavable biotinylation reagent for selecive enrichment ofazide-tagged biopolymers via SPAAC reaction.
Extraordinary strength of the streptavidin-biotin interaction allowsfor efficient capturing of even highly dilute targets; however, it makesrecovery of proteins from affinity resins challenging. Dde Biotin-DBCOprobe eliminates a major limitation of the streptavidin-biotin affinitypurification. This reagent contains a biotin moiety linked to an alkynemoiety through a spacer arm containing a Dde linker. Capturedbiomolecules can be efficiently released, typically >90%.
名稱：Diazo Biotin-DBCO
英文簡(jiǎn)介：
A sodium dithionite (Na2S2O4) cleavable biotinylation reagent forselecive enrichment of azide-tagged biopolymers via SPAAC reaction.
Diazo Biotin-DBCO allows capture of azide-tagged biomolecules via theCu-free click reaction under mild aueous buffer conditions. Thisreaction possesses extreme biopatibility, such that the plementaryreagents can form covalent bonds within living organisms. Biotinylatedprotein targets can be selectively captured through conventionalaffinity purification protocols that exploit the biotin - streptavidininteraction. Captured protein targets can be released under mildconditions with 50 mM sodium dithionite solution, and identified by massspectrometry, ELISA, dot blot, or Western blot applications.
名稱：DBCO-S-S-PEG3-Biotin
英文簡(jiǎn)介：
A cleavable biotinylation reagent for specific enrichment azide-tagged biomolecules.
DBCO-S-S-PEG3-Biotin enables simple and efficient biotin labeling ofazide-tagged antibodies, proteins and other macromolecules. The DBCOgroup reacts with an azide to produce an stable triazole, which is alsoreferred to as the Cu(I)-free or strain-promoted click reaction. Thehydrophilic PEG spacer arm imparts water solubility and reducesaggregation of labeled proteins stored in the solution. The integraldisulfide bond enables the biotin group to be cleaved from the labeledmolecule using DTT or other reducing agents.
名稱：Carboxyrhodamine 110 DBCO
英文簡(jiǎn)介：
A green fluorescence-emitting probe with excitation/emission maximum of501/523 nm
DBCO-activated Carboxyrhodamine110 dye reacts with azides to produce astable triazole, also referred to as the Cu-free click reaction, anddoes not reuire Cu-catalyst or elevated temperatures. This reaction isextremely selective and biopatible, such that the plimentaryreagents can form a covalent bond within richly functionalizedbiological systems, and, in some cases, living organisms.
名稱：Sulforhodamine B DBCO
英文簡(jiǎn)介：
A red fluorescence-emitting probe with excitation/emission maximum of568/585 nm
DBCO-activated Sulforhodamine B dye reacts with azides to produce astable triazole, also referred to as the Cu-free click reaction, anddoes not reuire a Cu-catalyst or elevated temperatures. This reactionpossesses extreme selectivity and biopatibility, such that theplimentary reagents can form covalent bonds within richlyfunctionalized biological systems, and, in some cases, living organisms.
名稱：TAMRA DBCO
英文簡(jiǎn)介：
An orange-to-red fluorescence-emitting probe with excitation/emissionmaximum of 554/567 nm
DBCO-activated TAMRA dye reacts with azides to produce a stabletriazole, also referred to as the Cu-free click reaction, and does notreuire Cu-catalyst or elevated temperatures. This reaction possessesextreme selectivity and biopatibility, such that the plimentaryreagents can form covalent bonds within richly functionalized biologicalsystems, and, in some cases, living organisms.
名稱：Sulforhodamine 101 DBCO
英文簡(jiǎn)介：
A red fluorescence-emitting probe with excitation/emission maximum of5/601 nm
DBCO-activated Sulforhodamine 101 dye reacts with azides to produce astable triazole, also referred to as the Cu-free click reaction, anddoes not reuire a Cu-catalyst or elevated temperatures. This reactionis extremely selective and biopatible, such that the plimentaryreagents can form covalent bonds within richly functionalized biologicalsystems, and, in some cases, living organisms.
名稱：Cy3 DBCO
英文簡(jiǎn)介：
An azide-reactive Cy3 dye for chemoselective labeling and detection ofazido-tagged molecules via the Cu-free click reaction.
名稱：Cy5 DBCO
英文簡(jiǎn)介：
A water-soluble, azide-reactive Cy5 dye for chemoselective labeling anddetection of azido-tagged molecules via the Cu-free click reaction.
名稱：Cy5.5 DBCO
英文簡(jiǎn)介：
An azide-reactive, water soluble Cy5.5 dye for chemoselective labelingand detection of azido-tagged molecules.
名稱：Cy7 DBCO
英文簡(jiǎn)介：
An azide-reactive, water-soluble Cy7 dye for chemoselective labelingand detection of azido-tagged molecules via the Cu-free click reaction.
名稱：DBCO-PEG4-Phosphoramidite
英文簡(jiǎn)介：
DBCO-PEG4-Phosphoramidite is a reagent for incorporation of DBCO moietyonto an oligonucleotide for subseuent copper-free click elaboration.
名稱：DBCO-PEG4-Hydroxyl
英文簡(jiǎn)介：
A modification reagent containing a long 4-unit polyethylene glycol(PEG4) spacer arm and a terminal primary hydroxyl group.
ThioLinker-DBCO
ThioLinker-DBCO
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