L-Azidohomoalanine,AHA,蛋氨酸代謝標記

L-Azidohomoalanine,AHA,蛋氨酸代謝標記，蛋氨酸，包含一個非常小的修飾氨基酸模擬，特別是疊氮基團可以飼養(yǎng)細胞和摻入蛋白質(zhì)蛋白質(zhì)的合成過程中。檢測采用化學選擇性結(jié)扎或“點擊"的疊氮化物和炔基或環(huán)辛炔的反應(yīng)。疊氮基改性蛋白檢測用熒光或生物素炔炔。

L-Azidohomoalanine,AHA,蛋氨酸代謝標記

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or &lduo;click &rduo; reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and patible with downstream LC-MS⁄MS and MALDI MS analysis.

Molecular weight	180.59 (HCl salt)
Chemical position	C4H9ClN4O2 (HCl salt)
CAS	942518-29-8
Solubility	Water, DMSO, DMF
Purity	>95% (H NMR)
Appearance	White crystalline
Storage conditions	-20C
Shipping conditions	Ambient temperature

 

L-Azidohomoalanine,AHA,蛋氨酸代謝標記

公司：西安凱新生物科技有限公司

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