Biolistic PDS-1000轉(zhuǎn)基因儀微粒導(dǎo)入儀 (基因槍)

美國(guó)BIO－RAD轉(zhuǎn)基因儀微粒導(dǎo)入儀 (基因槍)：Biolistic PDS-1000

Biolistic PDS-1000/He 基因槍系統(tǒng)將微米大小的、包被有核酸的金或鎢微粒加速到所需要的速度，從而將基因?qū)爰?xì)胞、組織或器官。這一過(guò)程既不依賴也不作用于細(xì)胞表面。系統(tǒng)利用高壓氦氣的轟擊，來(lái)推動(dòng)攜帶有微粒的塑料大顆粒載體盤朝目標(biāo)細(xì)胞方向加速。推進(jìn)大顆粒載體的氦氣壓力由所使用的不同破裂盤決定。破裂盤是一種可在特定壓力下破裂的塑料密封體。終止屏可阻止大顆粒載體，使微?？梢酝ㄟ^(guò)和轉(zhuǎn)染靶細(xì)胞。為了提高這一進(jìn)程的效率，可抽空轉(zhuǎn)化室，使壓力低于大氣壓力，從而當(dāng)微粒打向靶細(xì)胞時(shí)可減
少其摩擦阻力。

Biolistic PDS-1000/He 系統(tǒng)能提供：
• 一種可重復(fù)的、轉(zhuǎn)化完整培養(yǎng)細(xì)胞的方法，幾乎無(wú)需對(duì)細(xì)胞進(jìn)行處理
• 用其它方法不能完成的，有特殊生長(zhǎng)需要的細(xì)胞轉(zhuǎn)化
• 實(shí)驗(yàn)過(guò)程的控制
• 高重復(fù)性

The Biolistic pds-1000 /He gene gun system accelerates micron-sized particles of gold or tungsten coated with nucleic acid to the desired speed to introduce genes into cells, tissues or organs.This process does not depend on or act on the cell surface.The system USES a blast of high-pressure helium gas to propel a large plastic carrier carrying tiny particles in a disk toward the target cell.The helium pressure to propel the large particle carrier is determined by the different rupture disks used.A rupture disc is a plastic seal that can break under certain pressures.The termination screen prevents the carrier of large particles, allowing the particles to pass through and transfect the target cells.To improve the efficiency of this process, the chamber can be evacuated so that the pressure is lower than the atmospheric pressure, which can be reduced when the particles hit the target cells Less frictional resistance

Biolistic pds-1000 /He system can provide:

• a repeatable method of transforming fully cultured cells with little or no cell processing

• cell transformation that cannot be accomplished by other methods and has special growth needs

• maximum control of the experimental process

• high repeatability