總蛋白測試盒

說明書下載QCPR.rar

分類名稱 : 代謝產(chǎn)物分析 產(chǎn)品介紹 :

測定方法   Bradford比色法（595nm）定量測定總蛋白的濃度。

產(chǎn)品說明   蛋白質(zhì)被稱為“生命的物質(zhì)基礎(chǔ)”，它是生命科學(xué)領(lǐng)域zui重要的大分子之一。蛋白質(zhì)由多肽氨基酸組成，它在生物體內(nèi)發(fā)揮著各種關(guān)鍵的作用。蛋白質(zhì)定量檢測是生命科學(xué)家常用的方法。簡單、直接、自動化的蛋白質(zhì)濃度檢測方法是zui為理想的。本公司生產(chǎn)的蛋白質(zhì)測試法是基于改進的考馬斯藍G的方法。顯色劑與蛋白質(zhì)經(jīng)過復(fù)雜反應(yīng)形成藍色，其顏色強度（595nm）與樣品中的蛋白質(zhì)濃度成正比。優(yōu)化配方大幅減少了原樣品中物質(zhì)的干擾以及對多肽的更為敏感。 

產(chǎn)品用途    直接檢測樣品蛋白質(zhì)的總含量。

產(chǎn)品特點    靈敏、準確：用10 mL樣品。測試范圍 0.06 – 1.0 mg/mL 蛋白質(zhì).簡單、高通量： 測試過程僅需添加工作試劑及讀取吸光度。易于自動化高通量測試，每天可檢測幾千份樣品。低干擾:葡萄糖、維生素、氨基酸、DNA、 RNA、鹽、 EDTA (< 12 mM) 、 苯酚 (< 50 mM) 、 尿素 (< 0.6 M) 、表面活性劑 (< 0.1%) 和SDS (< 0.1% SDS)均不影響測試。多功能性: 測試可用96孔板或比色皿進行。

儲藏條件：室溫運輸 。試劑和標準品分別在室溫和 -20°C 下保存。

Description   The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. our QuantiChrom^TM protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides. 

Applications   Direct Assays: total protein concentration.

Key features     Sensitive and accurate. Use 10 mL samples. Detection range 0.06 – 1.0 mg /mL protein in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (< 0.1%) and SDS (< 0.1% SDS) do not interfere in the assay.Versatility: assays can be executed in 96-well plate or cuvet.

Storage conditions. The kit is shipped at room temperature. Store the reagent at room temperature and standard at -20°C, respectively. Shelf life of at least 6 months (see expiry dates on labels).

編號：QCPR-048,QCPR-500

測試：48T,500T