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分類名稱 : 代謝產物分析 產品介紹 :
測定方法 快速生物發(fā)光法測定組織或細胞中ADP：ATP的比例。檢測時間：20分鐘。運輸：冷運。儲存：-20°C。
Description Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis. our EnzyLightTM ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediay reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration.In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. Due to a special formulation of the reagent system which greatly stabilizes the light signal generated by the luciferase reaction, the luminescence from the initial ATP measurement remains stable throughout this assay. Therefore, the second light intensity measured represents the total ADP and ATP concentration in the sample.This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
Key features Safe. Non-radioactive assay.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Applications Apoptosis and Necrosis determination in cells.Cell proliferation: effects of cytokines, growth factor, nutrients.Drug discovery: high-throughput screening for anticancer drugs.
Storage conditions: store all reagents at -20°C. This product is shipped on dry ice. Shelf life of at least 6 months.
其他 :
編號：ELDT-048，ELDT-100
測試：48T,100T
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