Product Overview
The EpiQuik™ Acetyl-Histone H4 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H4 acetylation and DNA in vivo efficiently. The entire procedure can be c o mpleted within 5 hours and produces far more superiorresults than any c o mpetitor kits. The EpiQuik™ Acetyl-Histone H4 ChIP Kit is suitable for c o mbining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
WHY CHOOSE
THE EPIQUIK™ ACETYL-HISTONE H4 ChIP KIT?
- The fastest procedure available, which can be finished within 5 hours.
- Strip microwell format makes the assay flexible: manual or high
throughput. - Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
Product Details
The EpiQuik™ Acetyl-Histone H4 ChIP Kit contains all reagents required for carrying out a suc c essful chromatin immunoprecipitation for acetyl-histone H4 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H4 antibody and a negative control normal mouse IgG. Chromatin from the cells is
extracted, sheared, and added into the microwell imm o b ilized with the antibody. DNA is released from the antibody-captured acetyl-histone H4protein-DNA c o mplex, reversed, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
| SCHEMATIC PROCEDURE: | |
Product Components
CP1
(Wash Buffer)
CP2 (Antibody Buffer)
CP3A (Pre-Lysis Buffer)
CP3B (Lysis
Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6
(Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease
Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5
mg/ml)*
Anti-Acetyl-Histone H4 (1 mg/ml)*
8-Well Assay Strips (with
Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User
Guide
* For maximum
recovery of the products, centrifuge the original vial after thawing prior to
opening the cap.
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