現(xiàn)貨人肺炎衣原體抗體(Cpn-Ab)ELISA檢測(cè)試劑盒全國:歡迎選購!,,到我司741653262,公司提供*進(jìn)口,國產(chǎn)elisa試劑盒,均為高性價(jià)比試劑盒高品質(zhì)elisa試劑盒,齊一生物科技有限公司提供的試劑盒受到了廣大科研單位的*肯定和認(rèn)同.*保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供的產(chǎn)品.
產(chǎn)品名稱:現(xiàn)貨人肺炎衣原體抗體(Cpn-Ab)ELISA檢測(cè)試劑盒
規(guī) 格:96T/48T
檢測(cè)標(biāo)本:血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測(cè)方法:ELISA
檢測(cè)類型:酶聯(lián)免疫夾心法
產(chǎn)品的用途:僅供科研究課題使用!
價(jià)格:電議,,到我司qysw@qiyibio. .齊一生物科技(上海)有限公司提供的試劑盒受到了廣大科研單位的*肯定和認(rèn)同。*保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供的產(chǎn)品。若有需要,我司將竭誠為您服務(wù)本試劑僅供研究使用目的:本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量。
實(shí)驗(yàn)原理:本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平。用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度。
試劑盒組成:
試劑盒組成 48孔配置 96孔配置 保存
說明書 1份 1份
封板膜 2片(48) 2片(96)
密封袋 1個(gè) 1個(gè)
酶標(biāo)包被板 1×48 1×96 2-8℃保存
標(biāo)準(zhǔn)品:1800ng/L 0.5ml×1瓶 0.5ml×1瓶 2-8℃保存
標(biāo)準(zhǔn)品稀釋液 1.5ml×1瓶 1.5ml×1瓶 2-8℃保存
酶標(biāo)試劑 3 ml×1瓶 6 ml×1瓶 2-8℃保存
樣品稀釋液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
顯色劑A液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
顯色劑B液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
終止液 3ml×1瓶 6ml×1瓶 2-8℃保存
濃縮洗滌液 (20ml×20倍)×1瓶 (20ml×30倍)×1瓶 2-8℃保存
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
注意事項(xiàng):
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請(qǐng)避光保存。
7. 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號(hào)組分不得混用。
10. 如與英文說明書有異,以英文說明書為準(zhǔn)。
計(jì)算:
以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),
在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD
值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)
準(zhǔn)曲線的直線回歸方程式,將樣品的OD值
代入方程式,計(jì)算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實(shí)際濃度。
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)與批見應(yīng)分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個(gè)月 齊一生物科技有限公司主要經(jīng)營進(jìn)口原裝Elisa試劑盒,種類齊全,價(jià)格實(shí)惠,購買我公司產(chǎn)品的新老客戶,可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù),可檢測(cè)人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本。全程提供。如有什么問題請(qǐng)給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議。
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