人雌二醇受體(ER)試劑盒齊一生物齊一生物的ELISA試劑盒是您的*選擇:每個(gè)試劑盒都經(jīng)過嚴(yán)格的質(zhì)檢控制,包括:板內(nèi)、板間實(shí)驗(yàn)精度,靈敏度,回收率,穩(wěn)定性等指標(biāo)的測定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測體系簡單而直接,可根據(jù)450 nm波長的讀值迅速計(jì)算定量結(jié)果。試劑盒中提供的檢測抗體能夠識別全長靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測結(jié)果更穩(wěn)定可靠
人雌二醇受體(ER)試劑盒操作方法
雙抗體夾心法
1.包被:用0.05MPH9.牰碳酸鹽包被緩沖液將抗體稀釋蛋白質(zhì)含量為1~10μg/ml.在每個(gè)聚苯乙烯板的反應(yīng)孔中加0.1ml,4℃隔夜.次日,棄去孔內(nèi)溶液,用洗滌緩沖液洗3次,每次3分鐘.(簡稱洗滌,下同).
2. 加樣:加一定稀釋的待檢樣品0.1ml于上述已包被之反應(yīng)孔中,置37℃孵育1小時(shí).然后洗滌.(同時(shí)做空白孔,陰性對照孔及陽性對照孔).
3. 加酶標(biāo)抗體:于各反應(yīng)孔中,加入新鮮稀釋的酶標(biāo)抗體(經(jīng)滴定后的稀釋度)0.1ml.37℃孵育0.5~1小時(shí),洗滌.
4. 加底物液顯色:于各反應(yīng)孔中加入臨時(shí)配制的TMB底物溶液0.1ml,37℃10~30分鐘.
5. 終止反應(yīng):于各反應(yīng)孔中加入2M硫酸0.05ml.
6. 結(jié)果判定:可于白色背景上,直接用肉眼觀察結(jié)果:反應(yīng)孔內(nèi)顏色越深,陽性程度越強(qiáng),陰性反應(yīng)為無色或極淺,依據(jù)所呈顏色的深淺,以“+”、“-”號表示.也可測OD值:在ELISA檢測儀上,于450nm(若以ABTS顯色,則410nm)處,以空白對照孔調(diào)零后測各孔OD值,若大于規(guī)定的陰性對照OD值的2.1倍,即為陽性.
人雌二醇受體(ER)試劑盒間接法
1.用包被緩沖液將已知抗原稀釋1~10μg/ml,每孔加0.1ml,4℃隔夜;
2.次日洗滌3次;
3.加一定稀釋的待檢樣品(未知抗體)0.1ml于上述已包被之反應(yīng)孔中,置37℃孵育1小時(shí),洗滌;
4.(同時(shí)做空白、陰性及陽性孔對照)于反應(yīng)孔中,加入新鮮稀釋的酶標(biāo)第二抗體(抗抗體)0.1ml;
5.37℃孵育35-60分鐘,洗滌;
6.’zui后一遍用DDW洗滌.
其余步驟同“雙抗體夾心法”的4、5、6.
發(fā)展前景
臨床測定技術(shù)的發(fā)展主要在于方法學(xué)的發(fā)展,而方法學(xué)的發(fā)展依托于試劑生產(chǎn)技術(shù)不斷進(jìn)步更新和型標(biāo)記物的應(yīng)用.分子生物學(xué)正在并zui終肯定會讓對整個(gè)生命科學(xué)有一個(gè)全面而*的認(rèn)識,其對免疫測定技術(shù)發(fā)展的影響也是直接而又有效的,對以前一些難以檢測的生物活性物質(zhì)的測定,并且大大提高了檢測的靈敏度和特異性.
應(yīng)用領(lǐng)域
ELISA法是免疫診斷中的一項(xiàng)新技術(shù),現(xiàn)已成功地應(yīng)用于多種病原微生物所引起的傳染病、寄生蟲病及非傳染病等方面的免疫診斷.也已應(yīng)用于大分子抗原和小分子抗原的定量測定,根據(jù)已經(jīng)使用的結(jié)果,認(rèn)為ELISA法具有靈敏、特異、簡單、快速、穩(wěn)定及易于自動化操作等特點(diǎn).不僅適用于臨床標(biāo)本的檢查,而且由于一天之內(nèi)可以檢查幾百甚上千份標(biāo)本,因此,也適合于血清流行病學(xué)調(diào)查.本法不僅可以用來測定抗體,而且也可用于測定體液中的循環(huán)抗原,所以也是一種早期診斷的良好方法.因此ELISA法在生物醫(yī)學(xué)各領(lǐng)域的應(yīng)用范圍日益擴(kuò)大,
可概括四個(gè)方面:
1、免疫酶染色各種細(xì)胞內(nèi)成份的定位.
2、研究抗酶抗體的合成.
3、顯現(xiàn)微量的免疫沉淀反應(yīng).
4、定量檢測體液中抗原或抗體成份.
PCB, interface, drawer, Qsym
Cable, y, portal, Qsym
Cable, cLLD, pipetting system, Qsym
HBV PCR REAGENT III
Bracket, rear, transparent panel, Qsym
mericon Horse Kit (24)
PyroMark Storage Box 13x8x5cm, Pyro Box for dust free and safe storage of tips in-between runs in PyroMark Q96 MD.
PyroMark Assay Design SW 2.0 All-in-one software for design of both PCR and sequencing primers
Fungi ITS2 Primers For PCR
PyroMark Q24 Plate (100) CE 24 well plate for samples, for use in PyroMark Q24. 100 plates in each package. CE-marked
QIAvac HiSpeed LS
Elution Plate (10)
Type-it CNV SYBR Green PCR +qC Kit (100)
QIAGEN-tip 2500 (25) 25 columns; 2.5 mg column capacity
QIAGEN Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers
Buffer P2 500 ml Lysis Buffer
QIAfilter Plasmid Midi Kit (100) 100 QIAGEN-tip 100, Reagents, Buffers, 100 QIAfilter Midi Cartridges
QIAprep Spin Miniprep Kit (250) For 250 high-purity plasmid minipreps: 250 QIAprep Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
Plate Rotor 2 x 96 Rotor for 2 QIAGEN 96-well plates, for use with QIAGEN Centrifuges
Buffer PE (concentrate) 100 ml Wash Buffer (5x concentrate for 500 ml buffer)
Buffer ASL (4 x 140 ml) 560 ml Stool Lysis Buffer
人血清總補(bǔ)體(CH50)ELISA試劑盒
人過敏毒素/補(bǔ)體片斷4(C4a)ELISA試劑盒
人補(bǔ)體片斷5a(C5a)ELISA試劑盒
人補(bǔ)體片斷3a(C3a)ELISA試劑盒
人凝血因子Ⅷ相關(guān)抗原(Ⅷ-Ag)ELISA試劑盒
人凝血因子Ⅸ(FⅨ)ELISA試劑盒
人凝血因子Ⅹ(FⅩ)ELISA試劑盒
人血管性血友病因子/瑞斯托霉素輔因子(VWF)ELISA試劑盒
人纖溶酶抗纖溶酶復(fù)合物(PAP)ELISA試劑盒
人凝血酶抗凝血酶復(fù)合物(TAT)ELISA試劑盒
人抗凝血酶受體(ATR)ELISA試劑盒
人凝血酶受體(TR)ELISA試劑盒
人第八因子相關(guān)抗原(FⅧAg)ELISA試劑盒
人血纖蛋白原(Fbg)ELISA試劑盒
人主要組織相容性復(fù)合體Ⅰ類(MHCⅠ/HLAⅠ)ELISA試劑盒
人抗凝血酶Ⅲ抗體(AT-Ⅲ)ELISA試劑盒
人血栓前體蛋白(TpP)ELISA試劑盒
人雌二醇受體(ER)試劑盒人淋巴細(xì)胞功能相關(guān)抗原3(LFA-3/CD58)ELISA試劑盒
人主要組織相容性復(fù)合體Ⅲ類(MHCⅢ/HLA-Ⅲ)ELISA試劑盒
人主要組織相容性復(fù)合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒
人載脂蛋白A1(apo-A1)ELISA試劑盒
人載脂蛋白B100(apo-B100)ELISA試劑盒
人白血病抑制因子受體(LIFR)ELISA試劑盒
人免疫球蛋白A(IgA)ELISA試劑盒
人免疫球蛋白G(IgG)ELISA試劑盒
人免疫球蛋白M(IgM)ELISA試劑盒
人纖溶酶原激活物抑制因子1(PAI-1)ELISA試劑盒
人纖溶酶原激活物抑制因子(PAI)ELISA試劑盒
人血纖蛋白原降解產(chǎn)物(FDP)ELISA試劑盒
人血栓調(diào)節(jié)蛋白(TM)ELISA試劑盒
人血小板衍生生長因子(PDGF)ELISA試劑盒
Valve, shutoff, V2, QX
Valve, solenoid, V2, QX
Sensor, Z, with cable, V2, QX
Board, motor control, Y, V2, QX
Reagent Holder, 1-bottle Holder for acmodating 1 bottle (5 ml). For use with QIAGEN Protease on the BioRobot MDx worktable
BGR Tischplatte P3041 Robotec(SN602)
RS 232 Servicecabel
96-well Thermostat Base Plate1.02
Cooling and heating system, BR3000
Centrifuge, strip adaptor set
Maintenance kit, annual, BR9600 (A)
Transport lock, EZ1xl
Hose w. quick lock socket, PyroQ96VW
Pulley, Idler Y-axis, Cxx
Rail, Z-axis 138 mm, Cxx
Rail, X-axis 444 mm, CQx
miScript Reverse Transcription Kit (10)
FastLane Cell Multiplex Kit (200)
QuantiFast Probe PCR Kit (400)
Solid State Relay
Transformer 2x115VAC O/P 2x11VAC
Kit, lock ring 36W incl. lockknob, RG3
Transformer, heater, RG3
Mm/Hs_MAPK1 control siRNA (20 nmol)
Serial connector USB, internal cable, RG
Heating block, PyroQ24
Heating plate, GSC
PCR Buffer,10 x (1,2/2) without MgCl2
Coupling, CPC, inline, male, 6mm
Coupling, X motor, BR8000
Lead screw - Y robot, PyroQ96MD
Protease Solvent 6x (10,2/15), Azid,KG
AIV-H5 RT-PCR REAGENT LE
Effectene Transfection Reag. Kit (0.3ml)
HBV PCR REAGENT II
Foam insert, rotor securing, QIAC
Rotor Locking Nut Set
IQ/OQ document, QS
Sensor, interlock2, with cable, QX
Belt, Y, V2, QX
Board, transport conversion, V2, QX
Underlay, vaccum manifold, v2.01
Control unit Y/Z, 4 channel
Maintenance kit, semi annual, BR9600 (E)
Round carrier for 12 Tubes 15ml
PyroMark Q24 User Manual
Mixer module w. top plate, PyroQ96ID
Sensor, solenoid, EZ1xl
Cable harness, x axis, C34
PCB, mainboard, QGY
End cap, Y-arm, Cxx
Fuse, 2A
Adapter, reagent tub plate, CX1
TANK UPPER BUFFER ASSY GS
miScript SYBR Green PCR Kit (200)
miScript Reverse Transcription Kit (50)
miRNeasy FFPE Kit (50)
Mother board ( micro board V 2.1 )
QuantiTect SYBR Green RT-PCR Kit (1000) For 1000 x 50 ul reactions: 25 ml QuantiTect SYBR Green RT-PCR Master Mix (providing a final concentration of 2.5 mM MgCl2), 0.5 ml QuantiTect RT Mix, 20 ml RNase-free water
QuantiFast SYBR Green RT-PCR Kit (2000)
Rotor-Gene SYBR Green PCR Kit (400) The Rotor-Gene SYBR Green PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time PCR and two-step RT-PCR using SYBR Green I.
Hose, cooling blower, RG3
Belt, toothed, source optics, RG3
Gasket, cooling flap, RG3
EPROM, IC main board, state F/W, RG3
Gasket, lower chamber, RG6
Flexitube siRNA 20 nmol W Modification
FlexiPlate siRNA - 0.25 nmol
Board, main, no microprocessor, RG6
Mikrowin
Cap - Vacuum bottle, PyroVW
Coupling, CPC, screw, male
Rack for Murex Reagents GE 14/15 (P1/3)
Hinge kit - Robot, PyroQ96MD
QIAsymphony Circulating NA Kit(48),cus,G
Lid with LED, silver, RGQ
PyroMark Computer (OPC), PyroQ96
Service kit, pipettor 8 ch w/tool, Cx8
Ipad 4
Tray, buffer bottles, roter,BR8000 v1.00
人雌二醇受體(ER)試劑盒Switch - Vacuum, PyroQ96VW
Interlock, IL1, EZ1xl
Home flag, Y-axis, Cx2
Motor, Y-axis, Cxx
Pulley, motor, Al16T2.5/16-2, Cxx
Motor, X-axis, Cxx
miRNeasy Serum/Plasma Kit (50) The miRNeasy Serum/Plasma Kit is designed for purification of cell-free total RNA - primarily miRNA and other small RNA - from small volumes of serum and plasma. For using the included miScript Primer Assay as internal control the miRNeasy Serum/Plasma Spike-In Control (#219610) has to be purchased.
miRNeasy Micro Kit (50) The miRNeasy Micro Kit (50) is for purification of total RNA including miRNA from small sample volumes of cells and tissue.
Kit, heater, FET, ICs, diode, cap, RG3
Heater assy incl. element, sensors, RG3
Damper, lid, RG3
Rotor hub with shaft, RG6
Impeller, heater fan, RGx
FlexiPlate siRNA - 1 nmol
AllStars Neg. siRNA AF 546 (20 nmol)
人血小板活化因子(PAF)ELISA試劑盒
人組織型纖溶酶原激活劑(t-PA)ELISA試劑盒
人組織因子(TF)ELISA試劑盒
人β血小板球蛋白/β血栓環(huán)蛋白(β-TG)ELISA試劑盒
人血小板因子4(PF-4/CXCL4)ELISA試劑盒
人淋巴細(xì)胞功能相關(guān)抗原2(LFA-2/CD2)ELISA試劑盒
人白三烯E4(LTE4)ELISA試劑盒
人D二聚體(D2D)ELISA試劑盒
人免疫球蛋白E(IgE)ELISA試劑盒
人髓系細(xì)胞觸發(fā)受體-1(TREM-1)ELISA試劑盒
人白細(xì)胞調(diào)節(jié)素(LR)ELISA試劑盒
人血小板生成素(TPO)ELISA試劑盒
人白血病抑制因子(LIF)ELISA試劑盒
人Salusin-α ELISA試劑盒
人胰島素受體底物2(IRS-2)ELISA試劑盒
人轉(zhuǎn)甲狀腺素蛋白(TTR)ELISA試劑盒
人晚期氧化蛋白產(chǎn)物(AOPP)ELISA試劑盒
人胰島淀粉樣多肽(IAPP)ELISA試劑盒
人糖化蛋白(GSP)ELISA試劑盒
人糖化白蛋白(GA)ELISA試劑盒
人甲狀腺結(jié)合球蛋白(TBG)ELISA試劑盒
人晚期糖基化終末產(chǎn)物(AGEs)ELISA試劑盒
人C多肽(CP) ELISA試劑盒
人晚期糖基化終末產(chǎn)物受體(RAGE/AGER)ELISA試劑盒
人胰高血糖素(GC)ELISA試劑盒
人胰多肽(PP)ELISA試劑盒
人胰島素自身抗體(IAA)ELISA試劑盒
人谷氨酸脫羧酶(GAD)ELISA試劑盒
人胸腺五肽(TP-5)ELISA試劑盒
人胸腺肽(Thymosin)ELISA試劑盒
人未磷酸化類胰島素生長因子結(jié)合蛋白-1ELISA試劑盒
人甲狀旁腺激素樣蛋白(PLP)ELISA試劑盒
人內(nèi)脂素/內(nèi)臟脂肪素(visfatin)ELISA試劑盒
人apelin 12(AP12)ELISA Ki
人葡萄糖依賴性胰島素釋放多肽(GIP)ELISA試劑盒
人胰島素原(PI)ELISA試劑盒
人胰島素受體β(ISR-β)ELISA試劑盒
人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒
人胰高血糖素樣肽1(GLP-1)ELISA試劑盒