齊一生物半胱氨酸蛋白酶抑制劑胱抑素C(Cys-C)試劑盒齊一生物科技(上海)有限公司專(zhuān)業(yè)經(jīng)營(yíng)ELISA試劑盒種類(lèi)齊全,到貨快、確保每一個(gè)ELISA試劑盒質(zhì)量。凡是購(gòu)買(mǎi)我公司ELISA試劑盒免費(fèi)代測(cè)、提供技術(shù)服務(wù)、5個(gè)工作日出實(shí)驗(yàn)結(jié)果。歡迎新老客戶(hù)前來(lái): 到貨快捷安全:專(zhuān)業(yè)的物流配送,專(zhuān)人全程跟蹤訂單,確保貨物安全、準(zhǔn)確、及時(shí)地送達(dá)地點(diǎn)
齊一生物半胱氨酸蛋白酶抑制劑胱抑素C(Cys-C)試劑盒類(lèi)別:酶聯(lián)免疫分析檢測(cè)試劑盒
規(guī)格:96T/48T
包裝:盒
檢測(cè)原理:采用雙抗夾心法
保質(zhì)期:六個(gè)月
產(chǎn)品保存條件:2-8°C
應(yīng)用范圍:僅供科研研究實(shí)驗(yàn)使用
樣品形式:血清/血漿/組織勻漿/細(xì)胞培養(yǎng)上清//其它生物液體(具體情況請(qǐng)咨詢(xún))
標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過(guò)程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測(cè)分泌性的成份時(shí),用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測(cè)細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬(wàn)/ml左右。通過(guò)反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱(chēng)取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測(cè),其余冷凍備用。
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MinElute Gel Extraction Kit (50) 50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Ni-NTA HRP Conjugate Horseradish-peroxidase-conjugated Ni-NTA (lyophilized, for 500 ml working solution)
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