齊一生物現(xiàn)貨成骨生長肽（OGP）試劑盒

齊一生物現(xiàn)貨成骨生長肽（OGP）試劑盒齊一生物科技有限公司產(chǎn)品的新老客戶，可享免費代測實驗服務(wù)，可檢測人源，小鼠，大鼠，豬，狗，猴，兔，羊，雞，牛，鴨子，魚，植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報價，可齊一生物科技有限公司.全國：歡迎選購！

齊一生物現(xiàn)貨成骨生長肽（OGP）試劑盒操作步驟
1.標準品的稀釋與加樣：在酶標包被板上設(shè)標準品孔10孔，在*、第二孔中分別加標準品100μl，然后在*、第二孔中加標準品稀釋液50μl，混勻；然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔，再在第三、第四孔分別加標準品稀釋液50μl，混勻；然后在第三孔和第四孔中先各取50μl棄掉，再各取50μl分別加到第五、第六孔中，再在第五、第六孔中分別加標準品稀釋液50ul，混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中，再在第七、第八孔中分別加標準品稀釋液50μl，混勻后從第七、第八孔中分別取50μl加到第九、第十孔中，再在第九第十孔分別加標準品稀釋液50μl，混勻后從第九第十孔中各取50μl棄掉。（稀釋后各孔加樣量都為50μl，濃度分別為9ng/ml， 6ng/ml，4ng/ml，2ng/ml， 1ng/ml）。
2.加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）、待測樣品孔。在酶標包被板上待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻。
3.溫育：用封板膜封板后置37℃溫育30分鐘。
4.配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用。
5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)5次，拍干。
6.加酶：每孔加入酶標試劑50μl，空白孔除外。
7.溫育：操作同3。
8.洗滌：操作同5。
9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘. 
10.終止：每孔加終止液50μl，終止反應(yīng)（此時藍色立轉(zhuǎn)黃色）。
測定：以空白孔調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。 測定應(yīng)在加終止液后15分鐘以內(nèi)進行

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Gentra Puregene Tissue Kit (4 g)
RGS·His Antibody (100 μg)    100 ug mouse anti-RGS(H)4 (lyophilized, with BSA, for 1000 ml working solution)
pQE-TriSystem Vector DNA    25 ug pQE-TriSystem Vector DNA
EasyXpress Linear Template Kit Plus (20)
EasyXpress Protein Synthesis Kit (20)    For 20 x 50 ul reactions: E. coli extract, reaction buffer, RNase-free water, and positive-control DNA
Ni-NTA Superflow Cartridges (1x5 ml)
Ni-NTA Superflow (25 ml)    25 ml nickel-charged resin (max. pressure: 140 psi)
Sensor, Proximity SME-8M, DCU
# LED, 4 μA (12), QX
Piston pin, connecting rod, TLyser lT
MinElute Reaction Cleanup Kit (250)    250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
HC2 Systems Training – On site    HC2 On site, 3 day course trains users (max. 1 participant) on the HC2 system (manual or automated) and includes trainer travel, time and materials.
#HLA-B*5701 excl. Taq (12)
#Washer Alignment Block, PTW1
Tischplatte Plato 3101 SI Immucor (9194)
High speed shaker, autom. plate, BR8000
Base,rotor motor,buff.track,BR8000 v1.00
Ni-NTA HisSorb 384 Plates, white (100)
REPLI-g Single Cell Kit (96)
REPLI-g Midi Kit (100)    DNA Polymerase, Buffers, and Reagents for 100 x 50 μl whole genome amplification reactions (typical yield 40 μg per reaction)
REPLI-g UltraFast Mini Kit (25)    DNA Polymerase, Buffers, and Reagents for 25 x 20 μl ultrafast whole genome amplification reactions
Filter, condensate bottle, auto vac, BR8
Fuse T5A, v1.00
Splashing Cover , Wash Station RCS
Vacuum manifold, 6s seal
Tubing coupling piece waste station tub.
Pneumatic spring, 125 Nm
Varistor S20 K250
Eprom and controller 4K15, Zent3
QIAxcel Rental
QIArent&go - Rent out fee QIAgility
Pyro Q96 MD, Premium Agreement    "Repair service delivery: onsite
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Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
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Board, motor control, X, QX
Krytox RFE Lubricant
Shim Stock
PCB, p&c, cooling device, Qsym
Cooling device, eluate drawer, Qsym
Handler, Qsym
PCB, xyz, portal, Qsym
QIAcube, Full Agreement, no PM    "Repair service delivery: onsite
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Pogo Stick Kit, AP196/98
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Assy, Capper Shuttle, AP96
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RGQ, Inspection Service    "The Annual Inspection Service, Rotorgene-Q is a Regional Repair Center equipment service, performed by a QIAGEN service specialist. 
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Guided Valid Support, QuantiPlex/OPHyres    The QIAGEN Quantiplex/Quantiplex HYres Guided Validation Support service includes all travel and labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the Quantiplex/Quantiplex HYres chemistry utilized in the process, review the Handbook and User Manual, as well as hands-on operation to match the lab’s forensic workflow. The Guided Validation service includes experimental design consulation, internal validation documentation support, and QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design and are not included in the cost of validation support.
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PyroMark Q24 MDx, Priority    PyroMark Q24 MDx, Priority includes the Pyromark Q24 MDx system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service and response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-pliant Field Service documents. (In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)
QIAamp 96 DNA Swab BioRobot Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
NeXtalStock Citric acid pH 4.0 (200)