胎兒血紅蛋白(HBF)試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個(gè)試劑盒都經(jīng)過(guò)嚴(yán)格的質(zhì)檢控制,包括:板內(nèi)、板間實(shí)驗(yàn)精度,靈敏度,回收率,穩(wěn)定性等指標(biāo)的測(cè)定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測(cè)體系簡(jiǎn)單而直接,可根據(jù)450 nm波長(zhǎng)的讀值迅速計(jì)算定量結(jié)果。試劑盒中提供的檢測(cè)抗體能夠識(shí)別全長(zhǎng)靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測(cè)結(jié)果更穩(wěn)定可靠
胎兒血紅蛋白(HBF)試劑盒操作步驟:
1.標(biāo)準(zhǔn)品的稀釋與加樣:在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在*、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在*、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻;然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻;然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻;混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉。(稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L)。
2.加樣:分別設(shè)空白孔(空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、待測(cè)樣品孔。在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl(樣品zui終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻。
3.溫育:用封板膜封板后置37℃溫育30分鐘。
4.配液:將30(48T的20倍)倍濃縮洗滌液用蒸餾水30(48T的20倍)倍稀釋后備用。
5.洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
6.加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。
7.溫育:操作同3。
8.洗滌:操作同5。
9.顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
10.終止:每孔加終止液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。
11.測(cè)定:以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度(OD值)。 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。
cGKI Kinase Inhibitor Peptide 1mg
Asp371 Tyrosinase [369-377] Peptide 1mg
Autocamtide-2 Peptide 1mg
Calcineurin Autoinhibitory Peptide 1mg
Ser25 PKC [19-31] Peptide 1mg
CaMKII [290-309] Inhibitory Peptide 1mg
CaMKII Substrate Peptide 1mg
PKA Inhibitor [6-22] Peptide 1mg
Protein Kinase C [19-35] Peptide 1mg
Tyrosine Protein Kinase Substrate Peptide 1mg
Tyr-Specific Protein Kinase Inhibitor Peptide 1mg
Elastase Substrate Peptide 1mg
Trypsin Substrate Peptide 1mg
Cathepsin L Substrate Peptide 1mg
Amylin Peptide 1mg
Beta Amyloid [1-40] Peptide 1mg
Beta Amyloid [1-42] Peptide 1mg
ACTH [1-10] Peptide 2mg
ACTH [1-13] Peptide 2mg
ACTH [1-14] Peptide 1mg
ACTH [1-16] Peptide 1mg
ACTH [1-17] Peptide 1mg
ACTH [1-24] Peptide 1mg
ACTH [1-39] Peptide 1mg
ACTH [1-39] Peptide (Rat) 1mg
ACTH [1-4] Peptide 5mg
ACTH [18-39] Peptide 1mg
ACTH [4-10] Peptide 2mg
Adipokinetic Hormone Peptide (Locust) 2mg
Adipokinetic Hormone-2 Peptide (Locust) 2mg
Adipokinetic Hormone-2 Peptide (Schistocera) 2mg
Adipokinetic Hormone Peptide (Schistocera) 2mg
Adrenomedullin [13-52] Peptide 1mg
Adrenomedullin [1-52] Peptide 1mg
Adrenomedullin [22-52] Peptide 1mg
Met-Enkephalin Peptide 2mg
Alpha-Endorphin Peptide 1mg
Fibrinogen Gamma Chain AGDV Peptide 5mg
S6 Kinase Substrate [229-239] Peptide 2mg
ALAL Linker Peptide 5mg
Allatostatin 1 Peptide (Cockroach) 2mg
Alytesin Peptide (Frog) 2mg
Alpha-MSH Peptide 5mg
Amylin [8-37] Peptide 1mg
Amylin [8-37] Peptide (Rat) 1mg
Amylin Peptide (Feline) 1mg
Amylin Peptide (Rat) 1mg
Angiotensin Converting Enzyme Inhibitor Peptide 10mg
Angiotensin 1 [Des-Asp1] Peptide 20mg
Angiotensin 1 Peptide 20mg
Angiotensin 2 [1-4] Peptide 10mg
Angiotensin 2 [3-8] Peptide 10mg
Angiotensin 2 [4-8] Peptide 10mg
Angiotensin 2 [Sar1 Ile8] Peptide 10mg
Angiotensin 2 Peptide 10mg
Angiotensin 3 Peptide 10mg
Angiotensin Peptide (Rat) 10mg
Angiotensinogen [1-14] Peptide 2mg
Anorexigenic Peptide 5mg
ANP [1-11] Peptide (Rat) 1mg
PCB 6 - Connection, PyroQ96MD 9019213 qiagen
Camera MX9, PyroQ96MD 9019187 qiagen
Buffer AVE + Azid 108 x (2/2),KG 1020953 qiagen
HSV Quantification Standards 1061096 qiagen
HBV Controls & Quantification Standards 1061119 qiagen
Software Pack. LiquiChip Policy Manager 9016743 qiagen
Transformer (230V), HS 9019867 qiagen
Riser block, 19.5 mm, QXT 9019865 qiagen
Power supply, high voltage, V2, QX 9019760 qiagen
Board, smart key, V2, QX 9019730 qiagen
Upgrade-Kit Shakerplatte autom BR8000 9013674 qiagen
Reagent holder, 5-trough 80 ml 9013610 qiagen
Maintenance kit, semi annual, BR9600 (B) 9013428 qiagen
Tubing set, with HSPS, BR9600 9013423 qiagen
Set Adjustment plate, Qsym 9019666 qiagen
Temperature Controller, EZ1xl 9019640 qiagen
Board, main assembly, HS 9019631 qiagen
O-ring, filter housing seal 2.5mm, CX1 9019601 qiagen
Power supply, QGX 9019586 qiagen
Slideout electrical tray (220V), C1x 9019583 qiagen
Sensor, detector home, RG3 9019429 qiagen
Motor, stepper no cable, detector, RG6 9019364 qiagen
Motor-stepper, rotordrive,sourceopt, RG6 9019363 qiagen
FlexiTube GeneSolution siRNA 1 nmol 1027416 qiagen
Photomultiplier with cable, RG6 9019337 qiagen
Rack for Murex Reagents VK54/55,VK 9234679 qiagen
Optic fibre, 550 mm, GSC 9020473 qiagen
Connector, tubing, 1/8 in to 6 mm tubing 9234552 qiagen
AllStars Neg. Control siRNA (20 nmol) 1027281 qiagen
MagAttract Suspension G (1,6/2),KG 1026883 qiagen
DyeEx 2.0 Spin Kit (10) 1026577 qiagen
Frame, cooling, BR3000, 0 9234262 qiagen
8 Wash Head 9233477 qiagen
FastLane Cell cDNA Buffer Set (14x250ml) 1062737 qiagen
Board, Z2 motor 9232909 qiagen
Board, option, non-CE 9232907 qiagen
HBV Quantification Standards 1061052 qiagen
Influenza A RT-PCR REAGENT 1061067 qiagen
Power Supply, RCS1 9016569 qiagen
Transportation box, BioSprint 96 9016537 qiagen
Vacuum Tray, plete, BR8 RD, UK 9016463 qiagen
BioRobot MDx Performance Upgrade Kit 9016267 qiagen
Temp. Sensor, PyroQ96 9019184 qiagen
Opto - plate lock open, PyroQ96MD 9019178 qiagen
Label, cover, main, RG6 9019879 qiagen
Cover, main, red, RG6 9019876 qiagen
Fuse, 4A, 250VAC, V2, QX 9019762 qiagen
Motor, Z, V2, QX 9019733 qiagen
Transport lock, V2, QX 9019726 qiagen
Cable set, cooling system, 200 9013404 qiagen
Rect. sealing cap, clear, incl. clips 9238803 qiagen
Base Plate VPT, PyroQ96VW 9019653 qiagen
Tip ejector, Cx2 9019626 qiagen
Pins, locating, Mix and Reagent set, CG1 9019603 qiagen
Lead, vac trap sensor, CX1 9019598 qiagen
Belt, X-axis assembly, CQx 9019573 qiagen
Clamp, cable, 3 wires 2 tabs, Cxx 9019546 qiagen
PCB, USB, QGY 9019533 qiagen
RISER VACUUM 16.25MM 9019518 qiagen
miRNeasy Mini Kit (50) 217004 qiagen
QuantiTect SYBR Green RT-PCR Kit (200) 204243 qiagen
Heating block assy 9019439 qiagen
Gasket, lower chamber, RG3 9019425 qiagen
Box, shipping with foam packing, RG3 9019416 qiagen
Connector, COM incl. int. cable, RG3 9019401 qiagen
Switch, mains voltage selector, RG6 9019360 qiagen
Flexitube siRNA 5 nmol 1027417 qiagen
LED, green (530), RG6 9019339 qiagen
EPROM, IC Motherbrd, state FW, RG6 9019331 qiagen
Linear guide - X, PyroQ24 9019237 qiagen
X-Assembly Turntable Card 9234673 qiagen
Cassette, HSPS, click'n'go 9234659 qiagen
Buffer AVE (Elution Buffer) (125/125),KG 1026956 qiagen
RNeasy 96 Plates (12) 1026905 qiagen
Probe, ceramic coat., 0.4mm, 158mm 9233674 qiagen
Mousepad BR 01/10 (WW) 9233630 qiagen
Motor - Gripper, PyroQ96MD 9019209 qiagen
Damper kit - Robot, PyroQ96MD 9019197 qiagen
Buffer AW2 with Azid (127/500),KG 1020955 qiagen
HBV PCR REAGENT III 1062791 qiagen
Spritzenpumpe mMBP 2021C P7xxx 9232903 qiagen
PG Influenza A RT-PCR Assay (48) Z 1061065 qiagen
Valve, release, auto vac, BR8000 9232900 qiagen
Software Package LiquiChip Analyzer 9016710 qiagen
Pipetting head, 8 ch. , CX1 9019891 qiagen
Lid with LED, red, RG6 9019881 qiagen
36-well High Profile Locking Ring 9019857 qiagen
IPC Hard Drive PCA6751, PyroQ96MD 9019837 qiagen
Notebook_V3 W7_32bit 9022832 qiagen
Tubing, syringe to connector, BR3000 9013703 qiagen
Membrane, Simritz (20) 9013485 qiagen
Round sealing cap, clear 9238805 qiagen
Cable, UV lamp volt. conv., long, EZ1xl 9019637 qiagen