低分子肝素(LMWH)ELISA檢測試劑盒新近推出多種雙抗夾心法ELISA(酶聯(lián)免疫吸附法)試劑盒,具有特異性高、檢測范圍廣、靈敏度高和變異系數(shù)小等優(yōu)勢,是定量檢測多種生物樣本中靶蛋白(如細(xì)胞裂解液、血漿、血清等)的理想工具。
有效期:6個月
預(yù)期應(yīng)用:ELISA法定量測定血清、血漿、細(xì)胞培養(yǎng)上清或其它相關(guān)生物液體中的含量.
說明
低分子肝素(LMWH)ELISA檢測試劑盒1.試劑盒保存:-20℃(較長時間不用時);2-8℃(頻繁使用時).
2.濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶.
3.中、英文說明書可能會有不*之處,請以英文說明書為準(zhǔn).
4.剛開啟的酶聯(lián)板孔中可能會含有少許水樣物質(zhì),此為正?,F(xiàn)象,不會對實驗結(jié)果造成任何影響.
實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、生物素化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色.顏色的深淺和樣品中的該指標(biāo)呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度(OD值),計算樣品濃度.
試劑盒組成及試劑配制
1. 酶聯(lián)板(Assay plate ):一塊(96孔).
2. 標(biāo)準(zhǔn)品(Standard):2瓶(凍干品).
3. 樣品稀釋液(Sample Diluent):1×20ml/瓶.
4. 生物素標(biāo)記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根過氧化物酶標(biāo)記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶.
6. 生物素標(biāo)記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根過氧化物酶標(biāo)記親和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 濃洗滌液(Wash Buffer):1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10. 終止液(Stop Solution):1×10ml/瓶(2N H2SO4).
醋酸鳥氨酸加壓素 Ornipressin Acetate
醋酸催產(chǎn)素(縮宮素) Oxytocin Acetate
醋酸普蘭林肽 Pramlintide Acetate
醋酸PT141 Bremelanotide (PT-141)Acetate
醋酸鮭降鈣素 Salcitonin Acetate
醋酸胰泌素 Secretin Acetate
醋酸舍莫瑞林 Sermorelin Acetate
醋酸生長抑素 Somatostatin Acetate
醋酸立特帕肽 Teriparatide Acetate
醋酸特利加壓素 Terlipressin Acetate
醋酸曲普瑞林 Triptorelin Acetate
醋酸齊考諾肽 Ziconotide Acetate
胸腺五肽 Thymopentin
胸腺肽α1 Thymosin α1
醋酸那法瑞林 Nafarelin Acetate
醋酸增血壓素 Hypertensin Acetate
醋酸苯賴加壓素 Felypressin Acetate
醋酸后葉加壓素(抗利尿激素) Vasopressin Acetate
醋酸布舍瑞林 Burserlin Acetate
醋酸胸腺素β4 Thymosin β4 Acetate
乙?;强s氨酸-3 (醋酸阿基瑞林 ) Acetyl Hexapeptide-3 (Argreline Acetate)
棕櫚酰五肽 Palmitoyl Pentapeptide
醋酸脂肽 Lipopeptide Acetate
醋酸M肽 Matrixyl Acetate
HA Epitope Tag Antibody, Agarose conjugate (2-2.2.14) 5 mL RESIN
HA Epitope Tag Antibody (2-2.2.14), 100 ug. 100 UG
Pierce HA Peptide 5 mg
Pierce NHS-Activated Agarose, Dry 1 g
Pierce NHS-Activated Agarose, Dry 5 G
Pierce NHS-Activated Agarose Spin Columns, 0.2mL 25 SPIN COLU
Pierce NHS-Activated Agarose Spin Columns, 2mL 5 SPIN COLUM
Pierce NHS-Activated Agarose Slurry 25 mL.
Sulfo-NHS-Acetate 100 mg
PITC (Edman's Reagent) 10/1 mL
Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) 100 mg
RBS 35 Concentrate 20 kg
RBS 35 Concentrate 1 kg
RBS 35 Concentrate 5 KGS
RBS-35 CONCENTRATE, 220 KG 220 KG
RBS pF Detergent Concentrate 1 kg
RBS pF Detergent Concentrate 5 KG
RBS Solid Detergent 5 KG
Pierce Biotin Quantitation Kit Kit
HABA 10 GM PKG.
EZ-Link Hydrazide-Biocytin 25 mg
EZ-Link Biocytin 100 mg
PMPI 50 mg
Tween-80 Surfact-Amps Detergent Solution 500 mL
CHAPS 100 GM
CHAPS 5 GM
Octyl-beta-Glucoside 5 GM
Sodium Dodecyl Sulfate (SDS), C12 500 GM
Triton X-100 Surfact-Amps Detergent Solution 1000 mL
Triton X-100 Surfact-Amps Detergent Solution 6/10 mL
Brij-35 Surfact-Amps Detergent Solution 6/10 mL AMPS
Tween-20 Surfact-Amps Detergent Solution 6/10 mL
Tween-20 Surfact-Amps Detergent Solution 1000 mL
NP-40 Surfact-Amps Detergent Solution 6 X 10 mL
Tween-80 Surfact-Amps Detergent Solution 6 X 10 mL
Tween-80 Surfact-Amps Detergent Solution 50 mL
Triton X-114 Surfact-Amps Detergent Solution 6 X 10 mL
Brij-58 Surfact-Amps Detergent Solution 6 X 10 mL
Surfact-Amps Detergent Sampler Kit
20X Borate Buffer 0.5 L
20X Modified Dulbecco's PBS Buffer 0.5 mL
20X Modified Dulbecco's PBS Tween-20 Buffer 0.5 L
20X Phosphate Buffered Saline 0.5 L
Octylthioglucoside (OTG) 5 GM
20X PBS Tween-20 Buffer 500 mL
20X TAE Buffer 500 mL
10X TBE Buffer 1 L
20X TBS Buffer 500 mL
20X TBS Tween-20 Buffer 0.5 L
10X Tris-Glycine SDS Buffer 1000 mL
10X Tris-Glycine Buffer 1 L
Sodium Dodecyl Sulfate (SDS), Lauryl 100 GM
Sodium Dodecyl Sulfate (SDS), Lauryl 1000 GM
20X Tris-HEPES-SDS Buffer 500 mL
BupH Phosphate Buffered Saline Packs 40 PACKS/PKG
BupH Modified Dulbecco's PBS Packs 40 PACKS/PKG
BupH Tris Buffered Saline Packs 40 PACKS/PKG
BupH Tris-Glycine-SDS Buffer Packs 40 PACKS/PKG
BupH Tris Buffered Saline Packs 10 PACK
BupH Tris-Glycine Buffer Packs 40 PACKS/PKG
BupH Carbonate-Bicarbonate Buffer Packs 40 PACKS/PKG
BupH Borate Buffer Packs 40 PACKS/PKG
BupH MES Buffered Saline Packs 10 PACKS
BupH Tris-HEPES-SDS Running Buffer 10 PACK
Pierce Iodination Reagent 1 g
Pierce Iodination Tubes 10 TUBES/PKG
Pierce Iodination Beads 50/PKG
Pierce Iodination Beads 250/PKG
Trifluoroacetic Acid (TFA), Sequencing grade 500 mL
Trifluoroacetic Acid (TFA), Sequencing grade 10/1 GM
Trifluoroacetic Acid (TFA), Sequencing grade 100 GM
Trifluoroacetic Acid (TFA), Sequencing grade 10 X 1 mL
Formic Acid, LC-MS Grade 10 X 1 mL
16% Formaldehyde (w/v), Methanol-free 10 X 1 mL
16% Formaldehyde (w/v), Methanol-free 10 X 10 mL
TNBSA Solution 100 mL
Biotin 1 g
Horseradish Peroxidase, Biotinylated 5 mg
NeutrAvidin Agarose 5 mL
NeutrAvidin Agarose 10 mL
High Capacity NeutrAvidin Agarose 5 mL
High Capacity NeutrAvidin Agarose 10 mL