小鼠碳酸酐酶(CA)檢測試劑盒英文名稱Mouse carbonic anhydrase,CA ELISA Kit價格:全國:,到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進口原裝,分裝,以及穩(wěn)定性強的國產(chǎn)ELISA試劑盒,性價比*.
小鼠碳酸酐酶(CA)檢測試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標準品的數(shù)量決定所需的板條數(shù).每個標準品和空白孔建議做復孔.每個樣品根據(jù)自己的數(shù)量來定,能使用復孔的盡量做復孔.標本用標本稀釋液1:1稀釋后加入50ul于反應孔內(nèi).
加入稀釋好后的標準品50ul于反應孔、加入待測樣品50ul于反應孔內(nèi).立即加入50ul的生物素標記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標板,迅速加入50ul終止液,加入終止液后應立即測定結果.
在450nm波長處測定各孔的OD值.
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卵黃氯化鈉瓊脂
卵磷脂吐溫-80營養(yǎng)瓊脂
卵磷脂吐溫-80營養(yǎng)瓊脂
馬鈴薯葡萄糖瓊脂
馬鈴薯葡萄糖瓊脂
馬鈴薯葡萄糖瓊脂
麥康凱瓊脂
麥康凱瓊脂
麥康凱瓊脂
麥康凱瓊脂
玫瑰紅鈉(虎紅)瓊脂培養(yǎng)基
玫瑰紅鈉(虎紅)瓊脂培養(yǎng)基
孟加拉紅瓊脂
平板計數(shù)瓊脂
平板計數(shù)瓊脂
平板菌落計數(shù)瓊脂
葡萄糖瓊脂
慶大霉素瓊脂
瓊脂,顆粒狀
瓊脂粉
瓊脂粉
瓊脂粉
瓊脂條
瓊脂Noble
瓊脂VRB
肉湯瓊脂培養(yǎng)基
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